In vitro propagation of medicinal and aromatic plants: the case of selected Greek species with conservation priority

Grigoriadou, Katerina; Krigas, Nikos; Sarropoulou, Virginia; Papanastasi, Katerina; Tsoktouridis, Georgios; Maloupa, Eleni

doi:10.1007/s11627-019-10014-6

Cited by 40 publications

(29 citation statements)

References 39 publications

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“…P. turczaninovii is one of the important species of flora of the Baikalian Region, and this species should be preserved as a valuable resource for future generations. Improving propagation, cultivation and seed storage conditions gives an opportunity to maintain plant biodiversity (Danova et al 2009;Grigoriadou et al 2011Grigoriadou et al , 2019Abeli et al 2020). Previously, tissue culture of P. patens was initiated by Priede and Kļaviņa (2011) from seeds collected from naturally grown population and in our study the same methodological approach was chosen for P. turczninovii.…”

Section: Discussionmentioning

confidence: 88%

Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Hanus-Fajerska

Kocot

Wiszniewska

et al. 2021

Plant Cell Tiss Organ Cult

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Pulsatilla turczaninovii is an important medicinal plant, valued for high ornamental value of melliferous flowers. We assessed the efficiency of reproduction under in vitro conditions and the ex situ growth capacity of this important representative of the world flora. The seed germination percentage was assessed, followed by determination of micropropagation rate and rooting efficiency. Then, the possibility of plant development in three consecutive growing seasons was assessed. The in vitro germination percentage was approximately 55%. The highest multiplication coefficient, amounting to 5.17, was obtained on modified MS medium supplemented with 2.5 mg L−1 2iP and 1.0 mg L−1 IAA. Our study provided unique insight on biochemical background of root regeneration in P. turczaninovii. In comparison with standard auxin-supplemented rooting medium, the treatment with 1.0 mg L−1 level of ethylene precursor ACC elevated rooting by about 20%. The total content of soluble sugars was proved to be biomarker of rhizogenesis in the studied species. Their concentration was positively correlated with rooting efficiency, while a level of phenolic was positively correlated with the length of regenerated roots, and their number per rosette. The cultivation of the acclimatized material was successfully carried out and was evaluated over three subsequent years. In the third year of cultivation, the plants entered the stage of generative development and most of them bloomed profusely.

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Section: Discussionmentioning

confidence: 88%

Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Hanus-Fajerska

Kocot

Wiszniewska

et al. 2021

Plant Cell Tiss Organ Cult

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“…Pulsatilla turczaninovii is one of the important species of this unique ora, and it should be preserved as a valuable resource for future generations. Improving propagation, cultivation and seed storage conditions give an ideal opportunity to maintain plant biodiversity, which is often the case of species described for its medicinal properties (Abeli et al 2020;Grigoriadou et al 2011Grigoriadou et al , 2019Danova et al 2009). To the important works in tissue cultures of Asiatic Pulsatilla species should be included those by Jin et al (2007) and Lin et al (2011) regarding Pulsatilla koreana because it produces different valuable bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

Propagation in Vitro and Experimental Field Cultivation ex Situ of Pulsatilla Turczaninovii Kryl. et Serg. (Ranunculaceae)

Hanus-Fajerska

Kocot

Koźmińska

et al. 2021

Preprint

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Pulsatilla turczaninovii is an important medicinal plant and a material also appreciated by gardeners for its high ornamental value. In this study we assessed the efficiency of reproduction under in vitro conditions of this important representative of the world flora. First, the germination percentage and the following plant development during three growing seasons were assessed, then we tested the possibilities of multiplication by in vitro culture methods. Our study provided new insight on biochemical background of generating adventitious roots in Pulsatilla considered difficult-to-rooting. The germination percentage of studied population reached about 55%. The highest multiplication coefficient of micro-rosettes in tissue culture, amounting to 5.17, was achieved using modified MS medium supplemented with 2.5 mg L-1 2iP and 1 mg L-1 IAA. We proved that in comparison with standard auxin-supplemented rooting medium, the treatment with addition of 1.0 mg L-1 ethylene precursor ACC elevated adventitious rooting by about 20%. The biochemical analyses revealed that total content of soluble sugars was the most impactful biomarker of adventitious rhizogenesis in studied species. Concentration of sugars was positively correlated with rooting efficiency, while the level of phenolic compounds was positively correlated with the root length and their number regenerated per single rosette.

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“…The importance of preserving various rare and endangered plant species used by humans has been discussed in many reports. For example, Grigoriadou et al [ 8 ] evaluated in vitro propagation protocols for 22 native medicinal and aromatic plants in Greece, which were assigned conservation priority, and Coelho et al [ 7 ] discussed various cryopreservation methods and assess the cryogenic effects for endemic plant species.…”

Section: Red-list Plant Speciesmentioning

confidence: 99%

“…It is associated, first, with the formation of callus, suspension, meristematic cultures, cultures of ovules, anthers and pollen, cryosurgery of tissues; secondly, the development of technologies for reproduction with the prospect of their further dedifferentiation and redifferentiation. Creating plant in vitro collections can be considered as a protection form of plants of natural flora, and an effective method of their ex situ biodiversity preservation, which is part of the overall strategy for plant protection [ 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Development in Micropropagation Techniques for Rare Plant Species

Chokheli

Dmitriev

Rajput

et al. 2020

Plants

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The current investigation aimed to present an overview of the conservation of biological diversity of rare and endangered plant species. Methods of biodiversity conservation as well as several overview recommendations for the preservation of various rare species have been considered. An overview of the taxa included in the red book has been presented on the example of the Russian Federation. Global and local codes and classifiers of plant rarity were also presented. Future prospects for the conservation of biological diversity and the creation and development of bioresource collections have been considered.

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In vitro propagation of medicinal and aromatic plants: the case of selected Greek species with conservation priority

Cited by 40 publications

References 39 publications

Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Propagation in Vitro and Experimental Field Cultivation ex Situ of Pulsatilla Turczaninovii Kryl. et Serg. (Ranunculaceae)

Recent Development in Micropropagation Techniques for Rare Plant Species

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