In Vitro Propagation of Oriental White Oak Quercus aliena Blume

Li, Qiansheng; Gu, Mengmeng; Deng, Min

doi:10.3390/f10060463

Cited by 10 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The suitability of the MS medium over WPM and B5 has also been reported by other workers, such as in Magnolia sirindhorniae (Cui et al, 2019 ), Aspilia africana (Okello et al, 2021 ), and Azadirachta indica (Bello et al, 2022 ). In contrast to our study, WPM was found as a suitable growth medium for woody tree species such as Achyrocline satureioides (Guariniello et al, 2018 ), Quercus aliena (Li et al, 2019 ), Hildegardia populifolia (Upadhyay et al, 2020 ), and Alangium salviifolium (Pandey et al, 2022 ).…”

Section: Discussioncontrasting

confidence: 79%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Casuarina equisetifolia is an important tree of the forest, cultivated in tropical and subtropical regions, providing fuelwood, land reclamation, dune stabilization, paper production, and nitrogen fixation. We have developed a systematic in vitro propagation protocol in C. equisetifolia using nodal segments (NS). Murashige and Skoog (MS) medium augmented with BA (5.0 μM) and NAA (0.5 μM) gave rise to a maximum of 32.00 ± 0.31 shoots per explant (S/E) with shoot length (SL) of 3.94 ± 0.02 cm, and a maximum of 70% regeneration potential (RP) was recorded after 8 weeks of post inoculation. For root induction, in vitro derived shoots were transferred to the nutrient medium consisting of a half-strength (½) MS medium augmented with 2.5 μM NAA, which produced a maximum of 12.68 ± 0.33 roots/shoot (R/S) with 3.04 ± 0.50 cm root length (RL) in 60% of culture after 6 weeks. Micropropagated plants with healthy shoots and roots were successfully acclimatized in vermicompost + garden soil + sand (1:2:1) and a maximum survival percentage of 95.1% was recorded. NS was taken from a 6-weeks-old in vitro derived plant of C. equisetifolia for synthetic seed production, and it was reported that CaCl2 · 2H2O (100 mM) + Na2-alginate (4%) resulted in clear and uniform beads. Furthermore, the maximum conversion of synthetic seeds into plantlets occurred over a period of 4 weeks of storage at 4°C. Scanning Electron Microscopy (SEM) revealed the formation of direct shoot buds without any intermediate callus formation. In addition, the chlorophyll and carotenoid contents of the direct regenerated and mother plant were compared. Similarly, RAPD and ISSR primers were used for genetic homogeneity assessment of the direct regenerated plants, where a total of 18 and 19, respectively, clear and reproducible bands with 100% monomorphism were recorded. The developed micropropagation protocol can certainly be used for large-scale multiplication and germplasm preservation of C. equisetifolia. It will also help in meeting the growing demands of C. equisetifolia in the forest industry.

show abstract

Section: Discussioncontrasting

confidence: 79%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…In contrast, when no exogenous auxin was applied, the rooting rate was only 7%. Other studies in woody species have also reported higher rooting rates with auxin: in Quercus aliena, the highest rooting rate (41%) was achieved with a combination of 4.92 µM IBA and 5 g L −1 activated carbon [26]. In the woody species Acacia confusa, 72% rooting rate was achieved by supplementing the MS culture medium with 4.92 µM IBA [27].…”

Section: Discussionmentioning

confidence: 74%

Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species

Guerra

Badilla²,

Cautin

et al. 2022

Plants

View full text Add to dashboard Cite

Citronella mucronata (C. mucronata), a tree species endemic to Chile, has become threatened in its natural habitat and is currently listed as vulnerable. Tree population parameters have deteriorated due to indiscriminate logging and other anthropogenic activities, warranting research on mass propagation as a means of recovery. This study, unprecedented for this native species, has developed a successful method for its micropropagation. The objective was to establish a protocol for in vitro propagation of C. mucronata to produce large quantities of high-quality seedlings in an accelerated plant acquisition process. The best results were achieved by growing explants on Murashige and Skoog (MS) basal culture medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 14.76 μM indole-3-butyric acid (IBA). Explant survival rate was 78 %, the average shoot length reached 3.2 cm, the number of lateral shoots was 3.9, and rooting rate was 60%. Furthermore, stimulation with red and blue light in a 1:2 ratio, supplemented with 14.76 μM IBA, improved the rooting rate to 93%. The survival rate of rooted explants reached 100% in the acclimatization stage when using peat and perlite substrate (1:1 v/v).

show abstract

“…Both on the agar medium and on the TIS, a lower number of new shoots was observed on the modified QL medium as compared to the WPM in this study. The WPM medium is widely used during the in vitro multiplication phase for woody species during the in vitro multiplication phase of the genus Quercus [ 60 , 61 ]. Compared to QL, the WPM formulation has a higher nitrogen content, an essential macronutrient, and a structural component in plants.…”

Section: Discussionmentioning

confidence: 99%

Successful In Vitro Shoot Multiplication of Quercus robur L. Trees Aged up to 800 Years

Chmielarz

Kotlarski

Kalemba

et al. 2023

Plants

View full text Add to dashboard Cite

The conservation of the genetic resources of old trees is crucial to their ecological role but is extremely difficult, especially for oak species (Quercus spp.) displaying recalcitrance in seed and vegetative propagation methods. Our study aimed to assess the regenerative potential of Quercus robur trees of different ages (up to 800 years) during micropropagation. We also aimed to determine how in vitro conditions can influence in vitro regeneration responses. Lignified branches collected from 67 selected trees were cultivated ex vitro in culture pots at 25 °C to obtain epicormic shoots (explant sources). The explants were cultivated on an agar medium supplemented with 0.8 mg L−1 6-benzylaminopurine (BAP) for at least 21 months. In a second experiment, two different shoot multiplication conditions (temporary immersion—RITA® bioreactor and agar medium) and two culture medium formulations (Woody Plant Medium and modified Quoirin and Lepoivre medium) were tested. The results showed that the mean length of the epicormic shoots obtained in a pot culture was a function of donor age and was similar among the group of younger trees (ca. 20–200 years), and varied between older trees (ca. 300–800 years). The efficiency of in vitro shoot multiplication strictly depended on the genotype. A sustainable in vitro culture (defined as survival after 6 months) was only possible for half of the tested old donor trees, even when they survived the first month of in vitro growth. A continuous monthly increase in the number of in vitro cultured shoots was reported in younger oaks and in some old oaks. We found a significant effect of the culture system and the macro- and micronutrient composition on in vitro shoot growth. This is the first report demonstrating that the in vitro culture can be successfully applied to the propagation of even 800-year-old pedunculate oak trees.

show abstract

In Vitro Propagation of Oriental White Oak Quercus aliena Blume

Cited by 10 publications

References 36 publications

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species

Successful In Vitro Shoot Multiplication of Quercus robur L. Trees Aged up to 800 Years

Contact Info

Product

Resources

About