In Vitro Propagation of Pyracantha angustifolia (Franch.) C.K. Schneid.

Kaviani, Behzad; Deltalab, Bahareh; Kulus, Dariusz; Tymoszuk, Alicja; Bagheri, Hamideh; Azarinejad, Taha

doi:10.3390/horticulturae8100964

Cited by 12 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…genotypes 'Kashan' and 'Hervy Azerbaijan'. Previous studies revealed that PGRs play a vital role in enhancing the in vitro propagation efficiency of many species belonging to the Rosaceae family [7,29,30], which corresponds with our results.…”

Section: Discussionsupporting

confidence: 92%

“…Usually, the optimum concentration of PGRs for maximum shoot multiplication and root induction is not only species-specific, but also varies between genotypes, cultivars, and explant types, which is related to the different content of endogenous hormones [7,17]. Interestingly, in the present study, the highest number of shoots, in both genotypes studied, was produced in the same culture medium, i.e., MS containing 2 mg•L −1 BAP and 0.4 mg•L −1 GA 3 .…”

Section: Discussionmentioning

confidence: 99%

“…In vitro propagation is acknowledged as an important and effective technique for the large-scale propagation of horticultural crops, as well as for overcoming problems caused by heterogeneous seed production. The success of in vitro propagation techniques depends on a number of factors, i.e., the type and concentration of plant growth regulators (PGRs), the plant's genotype, the medium type, and explant parameters, which should be carefully optimized [7,8]. Micropropagation techniques have been utilized for several rose species [1,[8][9][10] and their hybrids [11].…”

Section: Introductionmentioning

confidence: 99%

“…Micropropagation techniques have been utilized for several rose species [1,[8][9][10] and their hybrids [11]. The successful use of conventional PGRs (auxins and cytokinins) for shoot multiplication and root production in Rosaceae members has been reported [7,10,11]. Cytokinins (6-benzylaminopurine-BAP, or kinetin-KIN) play a pivotal role in micropropagation by promoting cell division and shoot formation, thereby stimulating the development of multiple shoots from explants.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In Vitro Shoot Multiplication and Rooting of ‘Kashan’ and ‘Hervy Azerbaijan’ Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications

Kaviani,

Deltalab,

Kulus

et al. 2024

Plants

Self Cite

View full text Add to dashboard Cite

The damask rose (Rosa damascena Mill.) is an ornamental–medicinal plant from the Rosaceae family, and its aromatic compounds and essential oils are applied globally in the food, cosmetic, and pharmaceutical industries. Due to its economic value, this research aimed to establish a protocol for an efficient, rapid, and cost-effective method for in vitro shoot multiplication and rooting of the R. damascena ‘Kashan’ and ‘Hervy Azerbaijan’ genotypes. Nodal segments (as primary explants) were cultured on the Murashige and Skoog (MS) medium with combinations of various plant growth regulators (PGRs) such as gibberellic acid (GA3), 6-benzylaminopurine (BAP), and indole-3-butyric acid (IBA), as well as a PGR-like substance, phloroglucinol (PG), vitamins such as ascorbic acid (AA), and activated carbon in the form of active charcoal (AC). For the establishment stage, 0.1 mg·L−1 PG, 0.2 mg·L−1 GA3, and 1 mg·L−1 BAP were added to the media. Secondary explants (nodal segments containing axillary buds produced from primary explants) were obtained after 30 days of in vitro culture and transferred to the proliferation media supplemented with different concentrations of BAP (0, 0.5, 1, 1.5, 2, and 2.5 mg·L−1) and GA3 (0, 0.1, 0.2, 0.4, 0.8, and 1 mg·L−1) together with 0.1 mg·L−1 PG and 20 mg·L−1 of AA. The rooting media were augmented with different concentrations of BAP and GA3 with 0.1 mg·L−1 of IBA, PG and 20 mg·L−1 of AA and AC. The results showed that the highest regeneration coefficient (4.29 and 4.28) and the largest number of leaves (23.33–24.33) were obtained in the explants grown on the medium supplemented with 2 mg·L−1 BAP and 0.4 mg·L−1 GA3 for the ‘Kashan’ and ‘Hervy Azerbaijan’ genotypes, respectively. Likewise, this PGR combination provided the shortest time until bud break (approximately 6.5 days) and root emergence (approximately 10 days) in both genotypes. The highest number of shoots (4.78 per explant) and roots (3.96) was achieved in this medium in the ‘Kashan’ rose. Stem and root lengths, as well as stem and root fresh and dry weights, were also analyzed. In most measured traits, the lowest values were found in the PGRs-free control medium. Rooted plantlets were transferred to pots filled with perlite and peat moss in a 2:1 proportion and were acclimatized to ambient greenhouse conditions with a mean 90.12% survival rate. This research contributes significantly to our understanding of Damask rose propagation and has practical implications for the cosmetic and ornamental plant industries. By offering insights into the manipulation of regeneration processes, our study opens up new possibilities for the effective production of high-quality plant material.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Shoot Multiplication and Rooting of ‘Kashan’ and ‘Hervy Azerbaijan’ Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications

Kaviani,

Deltalab,

Kulus

et al. 2024

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…As there are few differences among the basal media used, it is quite difficult to identify the exact nutrient that promotes the growth in MS, but this could be the high level of NH 4 NO 3 present in MS, which is four times higher than that in QL and WPM. In general, MS is the most widely used medium to support tissue culture across a broad range of plant species [39][40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Duboisia Species via Shoot Tip Meristem

Xue,

Hiti-Bandaralage,

Jambuthenne

et al. 2023

Horticulturae

View full text Add to dashboard Cite

Duboisia is an Australian native, commercially valuable for tropane alkaloid extraction. Clonal propagation of elite selections is essential to establish highly productive plantations. The current propagation system using stem cuttings is proven to be inefficient, prompting the industry to seek a more efficient and effective propagation tool. Tissue culture is a cost-effective alternative for mass propagation of true-to-type plants, particularly ideal for propagating elite Duboisia selections. In this context, attempts were made to develop a commercially viable high throughput micropropagation system for three Duboisia species: Duboisia myoporoides, Duboisia leichhradtii and Duboisia hopwoodii. Various nutrient media, hormone combinations and incubating conditions were tested to optimise each stage of the micropropagation pipeline. The findings revealed that the tissue culture media composition and hormone requirements are species-specific. With the optimised conditions, an efficient tissue culture system was developed, achieving successful meristem induction and multiplication. Species-specific rooting protocol optimisation resulted in 100% rooting for D. myoporoides and D. leichhardtii, and 70% rooting for D. hopwoodii. Furthermore, an optimised acclimatisation protocol supported 100% survival of D. myoporoides and D. leichhardtii and 80% of D. hopwoodii plantlets. This study, for the first time, demonstrated the capacity of successful meristem culture of three Duboisia species, establishing the foundation for high throughput micropropagation of Duboisia species.

show abstract