In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant

Abstract

Background Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. Results Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. Conclusions This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement.

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

“…Half strength MS along with IAA 2.0 mg l À1 exhibited highest mean number of roots per shoot (11.40) and increased root length to the maximum (4.03 cm) (Figure 1d), while other concentrations of IAA caused antagonist effect on rhizogenesis in vitro in D. unguis-cati. Our results are in accordance with the studies carried out on Cichorium intybus [35], Digitalis lanata [36], Prosopis laevigata [37] and Securidaca longipedunculata [38]. The superiority of IAA over other auxins for induction of rhizogenesis has also been reported in Vanda pumila [39], Dendrobium chryseum [40], Phyllanthus tenellus [34].…”

Effect of nutrient media enhanced with plant-growth regulators on indirect somatic embryogenesis induction for the tissue culture of Digitalis purpurea

“…The effective superiority of one auxin over another within the nutrient media was attributed to the effective absorption and mobilization of the growth regulator or the rapid mobilization at the targeted sites [36]. The results of this study agree with those of Lijalem and Feyissa [37], which showed that different groups of growth regulators showed different responses in the formation of somatic embryos in Securidaca longipedunculota. They are also in agreement with the results of Gural et al [29] on Digitalis davisiana Heywood, showing that the presence of significant differences in the renewal of shoots depending on the explant type, plant type, hormonal combination and components of the basal medium, and the timing and requirements of growth regulators for the growth of somatic embryos differs according to the studied species [38].…”

High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

“…Interestingly, we noticed maximum shoot proliferation (72.2%) from node explant cultured onto MS forti ed with BAP @ 1 mg L -1 . Similar trends of ndings were also observed in Securidaca longipedunculata (Lijalem et al, 2020) and Trichosanthes dioica (Tiwari et al 2010). The nodal segment is considered to be the best explant type when compared to other types for initiation of direct shoot regeneration and proliferation and also requires only a lesser concentration of cytokinin, according to the Algabri et al survey (2019).…”