In vitro propagation of the endangered orchid Laelia speciosa

Abstract

Laelia speciosa is an endangered epiphytic orchid. The effects of various media components on germination of L. speciosa were evaluated. Pods were collected at 4, 7, and 9 months following hand-pollination, and seeds were germinated on Murashige and Skoog (MS) media with 30 g l -1 sucrose and five concentrations (0.0, 0.04, 0.22, 0.44, and 2.22 lM) of benzyladenine (BA) under light and dark conditions. Gibberellic acid (GA 3 ; 0.0, 0.29, 1.44, 2.89, 14. 43 and 28.87 lM) with naphthaleneacetic acid (NAA; 0.0, 0.54, 1.34, 2.69, and 5.37 lM) were evaluated for in vitro subcultivation. MS medium with 30 g l -1 sucrose was effective for germination. The effects of BA and light on germination of L. speciosa seeds differed with pod maturity. All mature seeds germinated using 0.44 lM BA and light. The highest frequency of germinated seedlings (60%) was obtained using mature seeds grown on MS medium without BA and under light conditions. For subculture, MS with 30 g l -1 sucrose, 2.69 lM NAA, and 0.29 lM GA 3 was effective. Plantlets of 5 cm in length were transplanted to the greenhouse, and a 77.5% of survival rate was obtained. A successful protocol for micropropagation by seed germination will contribute to the development of a sustainable management program for L. speciosa.

In vitro germination and development of two endangered endemic Colombian orchids Cattleya mendelii and Cattleya quadricolor

“…A higher proportion of NAA than GA 3 produced more and longer roots and longer leaves for both species grown in MS. For another Neotropical orchid, Laelia speciosa, GA 3 produces longer seedlings (Ávila-Díaz et al 2009). Here, high concentrations of GA 3 resulted in shorter leaves and roots than those of plants incubated in medium without this phytohormone.…”

“…In this respect, the in vitro propagation of vascular plants is a useful method that can be utilized for commercial or for conservation purposes as an alternative that might help in decreasing the extractive pressure on natural populations of endangered plants (Rubluo et al 1993, Arditti & Krikorian 1996, Buyun et al 2004, Lo et al 2004, Santos-Hernández et al 2005, Ávila-Díaz et al 2009). However, the in vitro propagation of orchids requires a species-specific method for massive and rapid production of these plants (Arditti 1977, Colli & Kerbauy 1993, Shimura & Koda 2003.…”

Establecimiento in vitro de protocormos de Prosthechea sp. bajo diferentes concentraciones de ácido naftalenacético

“…con 0,5 mg/l -1 y en Melissa officinalis Lindl. con 0,09 mg/l -1 , el cual fue capaz de mejorar la producción de brotes foliares, aunque no promovió rizogénesis (Ávila-Díaz et al, 2009;Da Silva et al, 2006;Pant & Pradhan, 2010).…”

“…con 0,2 mg/l -1 de ANA y en Cymbidium sp. con 0,1 mg/l -1 de ANA (Ávila-Díaz et al, 2009;Zhao et al, 2008).…”

“…A continuación se forma una estructura tuberizada (o masas de células indiferenciadas) llamada protocormo, con apariencia esférica ovoide y de color verde (fase 2). Siempre posee micorriza sobre la cual, eventualmente, se desarrollarán los primordios caulinares y radicales (fases 3 y 4) (Ávila-Díaz et al, 2009).…”