In vitro propagation of the elite species plant Pluchea lanceolata: Assessment of genetic stability by random amplified polymorphic DNA (RAPD) analysis

Abstract

In vitro produced plants were transferred to sterilized garden soil: compost (1:1) and then transferred to green house for hardening. Genetic stability of mother plant and the regenerants produced in vitro was assessed by random amplified polymorphic DNA (RAPD). In randomly selected plant material (mother plant) and its regenerants, 87 scorable bands were generated by four different primers, showing monomorphism with the mother plant. Thus, molecular analysis reveals that the micropropagation system described is a reliable method for propagation of P. lanceolata.