In vitro propagation of the ornamental bromeliad Acanthostachys strobilacea (Schult. f.) Klotzsch via nodal segments

Santos, Daniela Soares; Tamaki, Vívian; Nievola, Catarina Carvalho

doi:10.1007/s11627-010-9325-4

Cited by 17 publications

(13 citation statements)

References 29 publications

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“…According to Santos et al (2010b), the use of MS nutritive solution to irrigate Acanthostachys strobilaceae (Schult. F.) Klotzsch enabled a better growth rate of the root system, during acclimatization.…”

Section: Resultsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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The tissue culture can contribute to the propagation of several economic species, such as the bromeliads. This research aimed at evaluating the auxins type and concentration in the in vitro and ex vitro rhizogenesis of Neoregelia concentrica bromeliad. N. concentrica shoots were induced in a growth medium with 15.0 µM of 6-benzylaminopurine, for 80 days, followed by sub-cultivation in phytoregulator-free medium, for 45 days. In the in vitro rhizogenesis, the shoots grew in a medium supplemented with indole-3-butyric acid (IBA) or naphthalene-acetic acid (NAA), at the concentrations of 0.0 µM, 1.0 µM, 2.0 µM, 3.0 µM and 4.0 µM. In the ex vitro rhizogenesis, the bases of shoots were immersed, for 60 minutes, in IBA or NAA solutions, at the concentrations of 0.0 µM, 5.0 µM, 10.0 µM and 15.0 µM. After immersion, the shoots were planted in plastic trays with vermiculite. At the end of each rhizogenesis method, the phytotechnical parameters analysis was carried out. For the in vitro rhizogenesis, a higher number of roots were observed when the shoots were cultivated in concentrations higher than 1.0 µM of NAA, when compared to the IBA. However, the rooting rate differed only at 30 days after the in vitro growth, with a higher root induction in the shoots grown with NAA. At 60 days, the rooting rate was higher than 90% and statistically similar in all treatments. In the ex vitro rhizogenesis, a better formation of the rooting system was observed when 5.0 µM of IBA was applied, with higher rooting averages and number of roots.

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Section: Resultsmentioning

confidence: 99%

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Martins

Schimildt

Alexandre

et al. 2013

Pesqui. Agropecu. Trop.

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“…& Schult.f.) Klotzsch aclimatizadas em substrato com irrigação de diferentes diluições do meio MS, apresentaram em média 95% de sobrevivência ao término de 240 dias (Santos et al 2010). No entanto, espécies de bromeliáceas propagadas in vitro também podem apresentar porcentagens de sobrevivência inferiores, tais como Dyckia maritima Baker, que apresentou 63% a 91% de sobrevivência em diferentes substratos após 120 dias (Silva et al 2008) e A. blanchetiana, para a qual 65% a 85% Rodriguésia 67(4): 1071-1081.…”

Section: Número De Raízesunclassified

Baixas concentrações de macronutrientes beneficiam a propagação in vitro de Vriesea incurvata (Bromeliaceae), uma espécie endêmica da Floresta Atlântica, Brasil

2016

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A cultura in vitro é uma ferramenta eficiente para a propagação de plantas de importância ecológica e econômica e permite o entendimento acerca de aspectos ecofisiológicos das espécies. Este estudo teve por objetivo avaliar a influência de diferentes concentrações de macronutrientes sobre o desenvolvimento in vitro e a sobrevivência ex vitro de plântulas de Vriesea incurvata, visando à conservação desta bromélia epifítica endêmica da Floresta Atlântica. A germinação in vitro foi avaliada aos 60 dias e as plântulas foram cultivadas por 180 dias em meio MS com 25 ou 50% dos macronutrientes, 25 ou 50% dos sais nitrogenados, bem como com 100% da formulação original do meio. As sementes apresentaram 95% de germinação. Em todos os tratamentos, houve 100% de sobrevivência das plântulas cultivadas in vitro. A redução de todos os macronutrientes ou dos sais nitrogenados mostrou-se benéfica, proporcionando maior comprimento da parte aérea e da raiz maior, maior número de folhas e de raízes, bem como maior massa fresca, além de ter propiciado 97% de sobrevivência das plântulas aclimatizadas ex vitro. Os dados obtidos permitem o estabelecimento de um protocolo de propagação in vitro de V. incurvata, com o objetivo de sua futura reintrodução no habitat natural. Palavras-chave: bromélias, conservação, micropropagação, nutrição mineral. AbstractIn vitro culture is an efficient tool for the propagation of plants of ecological and economic importance and allows the understanding about ecophysiological aspects of the species. The objective of this study was to assess the influence of different macronutrient concentrations on in vitro development and ex vitro survival of Vriesea incurvata plantlets, aiming the conservation of this endemic epiphytic bromeliad from the Atlantic Forest. The in vitro germination was assessed at 60 days and the plantlets were cultivated for 180 days on MS medium with 25 or 50% of the macronutrients, 25 or 50% of the nitrogen salts, or with 100% of the original formulation of the medium. The seeds showed 95% germination. In all treatments, there was 100% survival of in vitro cultivated plantlets. The reduction of all macronutrients or of the nitrogen salts was beneficial, and permitted a greater length of the aerial part and of the major root, a higher number of leaves and roots, and more fresh mass, as well as allowed 97% survival of ex vitro acclimatized plantlets. The obtained data allow the establishment of a protocol for in vitro propagation of V. incurvata, aiming its future reintroduction into the natural habitat.

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“…Although light intensity may have a significant influence on the ex vitro growth rate of bromeliads, as already verified by Aragón et al (2012), previous studies have focused on potting medium type or mineral supplementation during acclimatization (Santos et al 2010;Huang et al 2011a;Silva et al 2012). In addition, acclimatization studies normally use the survival rate as the main measurement of successful acclimatization.…”

Section: Introductionmentioning

confidence: 93%

Chlorophyll a fluorescence and growth of Neoregelia concentrica (Bromeliaceae) during acclimatization in response to light levels

Martins

Schimildt

Alexandre

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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Tissue culturing is widely used for the clonal propagation of several plant species. However, high mortality rates may occur during the acclimatization phase due to the sudden, dramatic changes in light levels. This study evaluated the effect of light level during the acclimatization of Neoregelia concentrica (Vellozo) LB Smith using chlorophyll a fluorescence and growth analysis. N. concentrica plants were multiplied and rooted in vitro on Murashige and Skoog medium and then transferred to plastic trays with individual cells containing vermiculite. The treatments consisted of the ex vitro cultivation of plants under light levels of 30, 50, 70, and full sunlight (100% light) for 28 d. Chlorophyll a fluorescence measurements were taken at 0 (in vitro), 7, 14, 21, and 28 d of acclimatization, and growth trait analyses were made at 28 d. The high irradiance under full sunlight (100% light) induced photoinhibition and reduced the growth rate. Plants grown under partial light (30, 50, and 70%) were less inhibited. A 50% reduction in light level during N. concentrica acclimatization produced rapid plant recovery and higher growth rates than full sunlight.

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In vitro propagation of the ornamental bromeliad Acanthostachys strobilacea (Schult. f.) Klotzsch via nodal segments

Cited by 17 publications

References 29 publications

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting

Baixas concentrações de macronutrientes beneficiam a propagação in vitro de Vriesea incurvata (Bromeliaceae), uma espécie endêmica da Floresta Atlântica, Brasil

Chlorophyll a fluorescence and growth of Neoregelia concentrica (Bromeliaceae) during acclimatization in response to light levels

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