In vitro propagation of Vitis vinifera L. cv. ‘Monastrell’

Pedro, Tania San; Peiró, Rosa; Villanova, Joan; Olmos, Antonio; Gisbert, Carmina

doi:10.1016/j.ejbt.2017.03.006

Cited by 17 publications

(10 citation statements)

References 14 publications

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“…Immature seeds were extracted by hand from grapes with a mean weight from 0.65 to 0.85 g (6.0 to 7.0 mm long × 3.0 to 3.5 mm wide), corresponding to stage 33 in Pierce and Coombe [ 49 ]. The seeds were surface sterilized and cut transversely, before subculturing them on embryogenesis induction medium 2 (EIM2) [ 12 ] - that contains salts and vitamins of the McCown Woody plant medium (DUCHEFA, The Netherlands), 4% sucrose, 0.01% polyvinylpyrrolidone-10 (PVP-10), 0.75% plant agar, 0.2% AC, and 0.90 μM TDZ (sterilized by filtration and added to the sterile medium) - or on EIM2/2 medium (equal composition of EIM2 except for TDZ: 0.45 μM).…”

Section: Methodsmentioning

confidence: 99%

Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

et al. 2017

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BackgroundSomatic embryogenesis is the preferred method for cell to plant regeneration in Vitis vinifera L. However, low frequencies of plant embryo conversion are commonly found. In a previous work we obtained from cut-seeds of a grapevine infected with the Grapevine leafroll associated viruses 1 and 3 (GLRaV-1 and GLRaV-3), high rates of direct regeneration, embryo plant conversion and sanitation. The aim of this study is to evaluate the usefulness of this procedure for regeneration of other grapevine varieties which include some infected with one to three common grapevine viruses (GLRaV-3, Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV)). As grapevine is highly heterozygous, it was necessary to select from among the virus-free plants those that regenerated from mother tissues around the embryo, (true-to-type).ResultsSomatic embryogenesis and plant regeneration were achieved in a first experiment, using cut-seeds from the 14 grapevine varieties Airén, Cabernet Franc, Cabernet Sauvignon, Mencía, Merlot, Monastrell, Petit Verdot, Pinot Blanc (infected by GFLV and GFkV), Pinot Gris, Pinot Meunier, Pinot Noir, Syrah, Tempranillo (infected by GFLV), and Verdil. All regenerated plants were confirmed to be free of GFkV whereas at least 68% sanitation was obtained for GFLV. The SSR profiles of the virus-free plants showed, in both varieties, around 10% regeneration from mother tissue (the same genetic make-up as the mother plant). In a second experiment, this procedure was used to sanitize the varieties Cabernet Franc, Godello, Merlot and Valencí Blanc infected by GLRaV-3, GFkV and/or GFLV.ConclusionsCut-seeds can be used as explants for embryogenesis induction and plant conversion in a broad range of grapevine varieties. The high regeneration rates obtained with this procedure facilitate the posterior selection of true-to-type virus-free plants. A sanitation rate of 100% was obtained for GFkV as this virus is not seed-transmitted. However, the presence of GLRaV-3 and GFLV in some of the regenerated plants showed that both viruses are seed-transmitted. The regeneration of true-to-type virus-free plants from all infected varieties indicates that this methodology may represent an alternative procedure for virus cleaning in grapevine.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1159-3) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

et al. 2017

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“…In the context of the project CGL2015-70843-R, "Valencí Blanc" (sample Vb-Pe0), which resulted in infection with GFkV and GLRaV-3, was sanitized through both meristem culture and somatic embryogenesis. Meristems (n = 35) from plants of "Valencí Blanc" were extracted using a binocular lent and cultured in vitro on plates (90 Â 15 mm) containing the medium MW, selected for "Monastrell" micropropagation [71] supplemented with 6-benzylaminopurine (BAP) at 1.8 μM. Low light conditions were used for the two first weeks of culture.…”

Section: Virus Analysis and Sanitationmentioning

confidence: 99%

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Gisbert¹,

Peiró²,

Pedro³

et al. 2018

Grapes and Wines - Advances in Production, Processing, Analysis and Valorization

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A great number of varieties have been described in grapevine; however, few of them are currently in use. The increasing concern on varietal diversity loss has encouraged actions for recovering and preserving grapevine germplasm, which represents valuable resources for breeding as well as for diversification in grapevine-derived products. On the other hand, it is expected that this important crop, which is distributed in warm areas worldwide, will suffer the climate changes. Therefore, it is also convenient the identification of intravarietal variability and the recovery of accessions well adapted to particular environments. In this chapter, we will contribute to highlight the importance of recovering ancient materials, the usefulness of SSR markers to determine their molecular profile, the importance to analyze their virus status, and the possibilities that offer biotechnological tools for virus sanitation and in vitro storage as a complement of field preservation. In this context, we have evaluated different grapevine accessions and developed in vitro culture protocols for micropropagation, sanitation, and storage grapevine cultivars. In this work, we report the results obtained for the historic variety "Valencí Blanc" (or "Beba") and the historic and endangered variety "Esclafagerres" ("Esclafacherres" or "Esclafacherris").

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“…Many researchers note that control measures of this disease are reduced to prevention: planting certified healthy plants, disinfecting tools between each plant, getting rid of any infectioned plants, checking the soil for GLFV before the start of a season, and using nematode-resistant rootstocks [8,18]. Viral disease management relies heavily on prevention/exclusion or the use of economic and / or environmental factors [19].…”

Section: Introductionmentioning

confidence: 99%

Effect of fungicide farmayod on agrotechnical and technological indicators of grapevine, on viral diseases and oidium

Sirotkina

2021

E3S Web Conf.

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The paper presents the study on the effect of Farmayod’s GR (100 g/l of iodine) spraying on vineyards of Cabernet Sauvignon and Baklanovsky varieties on the degree of viral and oidium prevalence as well as on agrobiological and technological indicators. According to the aggregate agrobiological and technological indicators, the best results on Cabernet Sauvignon variety were obtained when the drug was used at a concentration of 0.06 %. On the Baklanovsky variety the best indicators were obtained at a drug concentration of 0.04%. Testing of plant samples for the presence of Grapevine fan leaf virus, Arabis mosaic virus and Oidium tuckeri showed that after two years of applying the drug, the prevalence of infected plants (P, %) with Grapevine fanleaf virus on the Cabernet Sauvignon cultivar varied from 0% (fungicide concentration 0.04 and 0.05 %) to 0.8 % (0.06 %) and 2.65 % (control). For Baklanovsky variety: Grapevine fanleaf virus - concentration 0.04 % - 1.8; 0.05 % - 0.4; 0.06 % - 2.0; control - 2.65 %. Arabis mosaic virus – 0; 0; 3.0; 12.1 %, respectively. Oidium tuckeri was 0 % in all variants with any drug concentrations. Control variant and later 80 % for 29.09.

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In vitro propagation of Vitis vinifera L. cv. ‘Monastrell’

Cited by 17 publications

References 14 publications

Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

Recovering Ancient Grapevine Varieties: From Genetic Variability to In Vitro Conservation, A Case Study

Effect of fungicide farmayod on agrotechnical and technological indicators of grapevine, on viral diseases and oidium

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