In vitro properties of apheresis platelet during extended storage in plasma treated with anandamide

Zhuang, Yi; Ren, Guijie; Li, Huiyu; Tian, Keli; Zhang, Yi; Qiao, Weiyang; Liu, Yan; Song, Yonghong; Zhu, Chaosheng

doi:10.1016/j.transci.2014.03.009

Cited by 7 publications

(5 citation statements)

References 37 publications

(53 reference statements)

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“…As a marker of platelet surface, the results of the use of CD61 are in agreement with other studies where the percentage of expression remained independent from the PAS used and the storage time [ 30 , 31 ]. The CD62P is described with inconsistent results when correlating the increased expression with post-transfusion increment [ 29 , 32 ]. In our experiment, the highest percentage of activation on day 1 was associated with the intense manipulation of the blood bag for its preparation, as described for human platelets.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the identification of intrinsic path markers (PS exposure, caspase marking) as well as extrinsic ones (quantification of cytochrome c, ΔΨm) have been used. The PS exposure is considered to be an activation marker with pro-coagulant activity, and also an apoptosis indicator [ 1 , 32 ]. In our study, no alterations during storage time were observed, however there is a greater expression in the control group on all the days of evaluation if compared to SSP+.…”

Section: Discussionmentioning

confidence: 99%

“…LDH is a marker of senescence. As the metabolic potential of platelets is exhausted, platelet lysis occurs as well as there lease of cytosolic components including LDH [ 10 , 32 ]. Studies in canine and human plasma PC and the use of PAS in human PCs demonstrate results that corroborate with those observed in our experiment [ 19 , 20 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

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In vitro properties of concentrated canine platelets stored in two additive solutions: a comparative study

Hlavac

Lasta²,

Dalmolin³

et al. 2017

BMC Vet Res

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BackgroundPlatelet transfusion therapy poses many challenges in veterinary clinical practice. Lack of readily available blood donors, short shelf-life, and inability to administer a sufficient number of platelets to meet a dog’s transfusion need are the major difficulties encountered. Platelet additive solutions are already in use at American and European human blood banks, showing to be a realistic alternative. This study compares the in vitro platelet function in plasma, Composol, or SSP+ during storage for 13 days. Platelet rich plasma-platelet concentrate with 35% plasma and 65% platelet additive solutions (Composol or SSP+) and a control group (100% plasma) were prepared. Swirling, platelet count, blood gases, metabolic variables, platelet activation markers, and apoptosis markers were analyzed on days 1, 5, 9 and 13.ResultsSwirling was well preserved and pH was acceptable (> 6.2) during storage for all platelet additive solutions units until day 9. SSP + units showed more stable pH and metabolic variables until day 13. Platelets in plasma showed higher glucose consumption than in Composol or in SSP+. The platelet additive solutions units showed better platelet metabolism maintenance, reduced glucose consumption and lactate production. The apoptotic markers were still low for 9 days in platelet concentrates with platelet additive solutions, suggesting the possibility to extend the shelf life with the use of SSP+ or Composol.ConclusionsOur findings suggest that the uses of Composol and SSP+ in canine platelet concentrates are potential alternatives in veterinary blood banks.Electronic supplementary materialThe online version of this article (10.1186/s12917-017-1236-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In vitro properties of concentrated canine platelets stored in two additive solutions: a comparative study

Hlavac

Lasta²,

Dalmolin³

et al. 2017

BMC Vet Res

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“…83 Furthermore, utility of anandamide, which can inhibit platelet apoptosis through the phosphatidylinositol 3-kinase/Akt pathway, 84 is able to reduce PS exposure and soluble P-selectin content in platelets after 7 days of storage although it has no effects during 5 days. 85 On the other hand, partial inhibition of caspase 3 activation by complement inhibitor compstatin during storage does not reduce PSL. 86 These studies suggest that apoptosis is ongoing during storage, but whether its inhibition is sufficient to preserve the viability of stored platelets requires additional investigation.…”

Section: Platelet Storage At Room Temperaturementioning

confidence: 99%

Mechanisms of platelet clearance and translation to improve platelet storage

Quach

Chen

2018

Blood

188

142

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Hundreds of billions of platelets are cleared daily from circulation via efficient and highly regulated mechanisms. These mechanisms may be stimulated by exogenous reagents or environmental changes to accelerate platelet clearance, leading to thrombocytopenia. The interplay between antiapoptotic Bcl-x and proapoptotic molecules Bax and Bak sets an internal clock for the platelet lifespan, and BH3-only proteins, mitochondrial permeabilization, and phosphatidylserine (PS) exposure may also contribute to apoptosis-induced platelet clearance. Binding of plasma von Willebrand factor or antibodies to the ligand-binding domain of glycoprotein Ibα (GPIbα) on platelets can activate GPIb-IX in a shear-dependent manner by inducing unfolding of the mechanosensory domain therein, and trigger downstream signaling in the platelet including desialylation and PS exposure. Deglycosylated platelets are recognized by the Ashwell-Morell receptor and potentially other scavenger receptors, and are rapidly cleared by hepatocytes and/or macrophages. Inhibitors of platelet clearance pathways, including inhibitors of GPIbα shedding, neuraminidases, and platelet signaling, are efficacious at preserving the viability of platelets during storage and improving their recovery and survival in vivo. Overall, common mechanisms of platelet clearance have begun to emerge, suggesting potential strategies to extend the shelf-life of platelets stored at room temperature or to enable refrigerated storage.

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“…Inhibition of p38MAPK decreases shedding of GPIba with an associated improvement in platelet survival in a mouse model, as well as a reduction in activation, improved metabolic markers, improved morphological parameters, and improved HSR in vitro . An endogenous endocannabinoid that inhibits platelet apoptosis via the PI3K/Akt pathway demonstrated improved pH, P‐selectin expression and HSR, but only by Day 7 of storage . Targeting other signaling pathways such as the one responsible for GPIIb‐IIIa activation has also been suggested .…”

Section: Liquid Stored Plateletsmentioning

confidence: 99%

Comprehensive review of platelet storage methods for use in the treatment of active hemorrhage

Milford

Reade

2016

Transfusion

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This review considers the various methods currently in use, or under investigation, for the storage of platelets intended for use in the treatment of active hemorrhage. The current standard practice of storing platelets at room temperature (RT) (20°C‐24°C) optimizes circulating time, but at the expense of hemostatic function and logistical considerations. A number of alternatives are under investigation. Novel storage media additives appear to attenuate the deleterious changes that affect RT stored platelets. Cold storage was originally abandoned due to the poor circulating time of platelets stored at 4°C, but such platelets may actually be more hemostatically effective, with a number of other advantages, compared to RT stored platelets. Periodically re‐warming cold stored platelets (temperature cycling, TC) may combine the hemostatic efficacy of cold stored platelets with the longer circulating times of RT storage. Alternatives to liquid storage include cryopreservation (freezing) or lyophilization (freeze‐drying). The former has had some limited clinical use and larger clinical trials are underway, while the latter is still in the preclinical stage with promising in vitro and in vivo results. The importance of platelet transfusion in the management of active hemorrhage is now well accepted, so it is timely that platelet storage methods are reviewed with consideration of not only their circulating time, but also their hemostatic efficacy.

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In vitro properties of apheresis platelet during extended storage in plasma treated with anandamide

Cited by 7 publications

References 37 publications

In vitro properties of concentrated canine platelets stored in two additive solutions: a comparative study

In vitro properties of concentrated canine platelets stored in two additive solutions: a comparative study

Mechanisms of platelet clearance and translation to improve platelet storage

Comprehensive review of platelet storage methods for use in the treatment of active hemorrhage

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