In vitro properties of gellan gum sponge as the dental filling to maintain alveolar space

Chang, Shwu Jen; Huang, Yi‐Ting; Yang, Shun-Chun; Kuo, Shyh-Ming; Lee, Ming-Wei

doi:10.1016/j.carbpol.2012.01.017

Cited by 63 publications

(25 citation statements)

References 18 publications

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“…To measure the porosity, the liquid displacement method was employed in which absolute ethanol was used as the liquid medium. 15 Before the experiment, the samples, pycnometer, and liquid medium were prewarmed to 25 C. Porosity was calculated using the following equation:…”

Section: Characterization Of Cb-hap and Pcl-coated Cb-hap Scaffoldsmentioning

confidence: 99%

Improvement of the compressive strength of a cuttlefish bone‐derived porous hydroxyapatite scaffold via polycaprolactone coating

Kim

Kang

Liu

2013

J. Biomed. Mater. Res.

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Cuttlefish bones (CBs) have emerged as attractive biomaterials because of their porous structure and components that can be converted into hydroxyapatite (HAp) via a hydrothermal reaction. However, their brittleness and low strength restrict their application in bone tissue engineering. Therefore, to improve the compressive strength of the scaffold following hydrothermal conversion to a HAp form of CB (CB-HAp), the scaffold was coated using a polycaprolactone (PCL) polymer at various concentrations. In this study, raw CB was successfully converted into HAp via a hydrothermal reaction. We then evaluated their surface properties and composition by scanning electron microscopy and X-ray diffraction analysis. The CB-HAp coated with PCL showed improved compressive performance and retained a microporous structure. The compressive strength was significantly increased upon coating with 5 and 10% PCL, by 2.09-and 3.30-fold, respectively, as compared with uncoated CB-HAp. However, coating with 10% PCL resulted in a reduction in porosity. Furthermore, an in vitro biological evaluation demonstrated that MG-63 cells adhered well, proliferated and were able to be differentiated on the PCL-coated CB-HAp scaffold, which was noncytotoxic. These results suggest that a simple coating method is useful to improve the compressive strength of CB-HAp for bone tissue engineering applications.

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Section: Characterization Of Cb-hap and Pcl-coated Cb-hap Scaffoldsmentioning

confidence: 99%

Improvement of the compressive strength of a cuttlefish bone‐derived porous hydroxyapatite scaffold via polycaprolactone coating

Kim

Kang

Liu

2013

J. Biomed. Mater. Res.

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show abstract

“…The stress versus strain curves were plotted based on the data obtained from the uniaxial tensile tests (Figure 3) and the elastic modulus was calculated from the slope of the fitted line to the quasi-linear domain of each curve. 11,30 Young's modulus, maximum stress and strain of the fabricated (P-sponge) and the commercial sponges are depicted in Figure 4. The results show that the CENEFOM, EYETEC and P-sponge have the elastic modulus of 42.25±2.84, 46.22±1.21 and 40±1.41 MPa, respectively.…”

Section: R E T R a C T E Dmentioning

confidence: 99%

RETRACTED: Fabrication and mechanical characterization of a polyvinyl alcohol sponge for tissue engineering applications

2013

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Polyvinyl alcohol (PVA) sponges are widely used for clinical applications, including ophthalmic surgical treatments, wound healing and tissue engineering. There is, however, a lack of sufficient data on the mechanical properties of PVA sponges. In this study, a biomechanical method is used to characterize the elastic modulus, maximum stress and strain as well as the swelling ratio of a fabricated PVA sponge (P-sponge) and it is compared with two commercially available PVA sponges (CENEFOM and EYETEC). The results indicate that the elastic modulus of the P-sponge is 5.32% and 13.45% lower than that of the CENEFOM and EYETEC sponges, while it bears 4.11% more and 10.37% less stress compared to the CENEFOM and EYETEC sponges, respectively. The P-sponge shows a maximum strain of 32% more than the EYETEC sponge as well as a 26.78% higher swelling ratio, which is a significantly higher absorbency compared to the CENEFOM. It is believed that the results of this study would help for a better understanding of the extension, rupture and swelling mechanism of PVA sponges, which could lead to crucial improvement in the design and application of PVAbased materials in ophthalmic and plastic surgeries as well as wound healing and tissue engineering.

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“…15 Therefore, we measured the water-uptake ability of COL-S and COL-S modified with various concentrations of FNG. Water-uptake ability is closely related to porosity.…”

Section: Resultsmentioning

confidence: 99%

Improvements of osteoblast adhesion, proliferation, and differentiation in vitro via fibrin network formation in collagen sponge scaffold

Kim

Lee

2013

J Biomedical Materials Res

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Collagen sponges (COL-S) are used as scaffolds to support osteoblasts and stimulate bone repair because of their flexibility, biocompatibility, and biodegradability. In this study, we added fibrin networks to COL-S scaffolds by using a fibrinogen (FNG) cross-linking reaction and evaluated the proliferation, differentiation, and adhesion of MG-63 cells on these scaffolds. The fibrin network that formed in COL-S with various concentrations of FNG was characterized with regard to morphology, porosity, and water-uptake ability. Successful fibrin network formation was observed by scanning electron microscopy (SEM). As the FNG concentration increased, network formation increased, but porosity and water-uptake ability were slightly reduced at high FNG concentrations. An MTS assay, DNA content assay, live/dead fluorescence assay, and SEM imaging showed that MG-63 cells attached and spread on COL-S and COL-S/FNG scaffolds, particularly on scaffolds modified using FNG. In addition, alkaline phosphatase (ALP) activity was significantly increased in cells cultured on scaffolds modified using 10, 40, and 80 mg/mL FNG. Thus, the addition of a fibrin network could increase the biocompatibility of COL-S for bone regeneration.

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In vitro properties of gellan gum sponge as the dental filling to maintain alveolar space

Cited by 63 publications

References 18 publications

Improvement of the compressive strength of a cuttlefish bone‐derived porous hydroxyapatite scaffold via polycaprolactone coating

Improvement of the compressive strength of a cuttlefish bone‐derived porous hydroxyapatite scaffold via polycaprolactone coating

RETRACTED: Fabrication and mechanical characterization of a polyvinyl alcohol sponge for tissue engineering applications

Improvements of osteoblast adhesion, proliferation, and differentiation in vitro via fibrin network formation in collagen sponge scaffold

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