In Vitro Properties of MEDI-4212, a Human Anti-IgE Antibody for the Treatment of Allergic Asthma.

Cohen, E. Suzanne; Eriksson, P.O.; Wachenfeldt, Karin von; Mendez, I de; Sims, Dorothy A.; Oakley, Sarah; Dobson, Claire; Persdotter, Sofia; Ekdahl, Helena; Azimi, Maryam; Alkner, U.; Monk, Phillip; Klakamp, Scott L.; OBrian, S; Lloyd, Clare M.; Carlsson, Mats; Coyle, Anthony J.

doi:10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5726

Cited by 3 publications

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“…The method was verified with results for discriminatory ability, precision, linearity and stability and compared well with the total IgE assay. The assay has also been used to demonstrate ex vivo the suppression of IgE by two anti-IgE mAbs, omalizumab and MEDI4212, in serum from individuals with a broad range of baseline IgE [12,13].…”

Section: Discussionmentioning

confidence: 99%

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Variability of Total and Free IgE Levels and IgE Receptor Expression in Allergic Subjects in and Out of Pollen Season

et al. 2015

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The inter-and intra-individual variability and seasonal variation of IgE, and high (FceRI)-and low-affinity (CD23) IgE receptor expression in blood of seasonal allergic rhinitis (SAR) subjects, is not well studied. Thirty-two otherwise healthy subjects with a history of SAR to birch pollen and a positive skin prick test to birch pollen were sampled three times out of the pollen season and three times during the pollen season. FceRI and CD23 expressions were analysed using flow cytometry. Total IgE was analysed using ImmunoCAP â and free IgE was analysed with a novel customised research assay using an IgG-FceRI-chimera protein coupled to ImmunoCAP as capture reagent, ImmunoCAP-specific IgE conjugate and ImmunoCAP IgE calibrators. The performance of the free IgE assay was compared well with the reference ImmunoCAP total IgE assay. The working range of the assay was 0.35-200 kU/l IgE. FceRI expression on basophils and CD23 expression on B cells showed low intrasubject variability both in and out of the pollen season (<10% CV). There was a small seasonal difference with lower total IgE levels (120 versus 128 kU/l; P = 0.004) and FceRI expression (283 versus 325 mean fluorescence intensity (MFI); P < 0.001) during the pollen season. IgE, FceRI expression and CD23 expression fulfilled biomarker and assay requirements of variability, and allergen exposure affected the biomarkers only to a minor degree. The free IgE assay may be used for measurement of free IgE levels in patients after anti-IgE antibody treatment.

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Section: Discussionmentioning

confidence: 99%

“…The accelerated stability testing indicated a stability of the IgG-FceRI-chimera protein ImmunoCAP up to 2 years when stored at 2-8°C. The assay was also tested to measure free IgE in serum from donors with different baseline IgE levels after the addition of varying concentrations of human anti-IgE mAbs [12,13].…”

Section: Methodsmentioning

confidence: 99%