In vitro properties of platelets stored in a small container for pediatric transfusion

Tynngård, Nahreen; Trinks, Marie; Berlin, Gösta

doi:10.1111/trf.12482

Cited by 3 publications

(4 citation statements)

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“…One limitation of this study was the use of half‐sized units to assess the impact of cold‐storage and UVC‐treatment. Whilst an effort was made to maintain the correct surface area to volume ratio by splitting each unit in two into half‐sized platelet bags , it is nonetheless a change that could reduce the generalisability to standard platelet storage. However, the results from the RT, cold and RT UVC‐PI platelet groups are in‐line with previously published data generated using standard platelet storage bags , thus indicating that the use of the half‐sized bags did not impact the validity of the results.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently both units were split and transferred into two half‐bags to create a total of four discrete platelet units. The integrated storage bag was heat sealed in half to ensure the appropriate surface area/volume ratio for gas exchange as that in a standard volume platelet concentrate . The units were then stored at room temperature (20–24°C) or in the cold (2–6°C), resulting in the following treatment groups: RT untreated, RT‐UVC, cold untreated and cold‐UVC ( n = 8 for each group).…”

Section: Methodsmentioning

confidence: 99%

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The impact of refrigerated storage of UVC pathogen inactivated platelet concentrates on in vitro platelet quality parameters

et al. 2018

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Background and Objectives Refrigeration (cold‐storage) of pathogen inactivated (PI) platelet components may increase the shelf‐life and safety profile of platelet components, compared to conventional room‐temperature (RT) storage. Whilst there is substantial knowledge regarding the impact of these individual treatments on platelets, the combined effect has not been assessed. Materials and methods Using a pool‐and‐split study design, paired buffy‐coat derived platelets in 70% platelet additive solution (SSP+; MacoPharma) were left untreated or PI‐treated using the THERAFLEX UV‐Platelets System (UVC; MacoPharma). Units from each pair were split and stored at room temperature (20–24°C) or cold‐stored (2–6°C) to yield RT, cold, RT‐UVC and cold‐UVC study groups (n = 8 in each group). In vitro quality and function was tested over 9 days. Results Cold‐storage of UVC‐treated platelets reduced glycolytic metabolism (glucose consumption and lactate production) compared to RT‐UVC units. Cold‐UVC platelets demonstrated complete abrogation of HSR by day 5, increased externalisation of phosphatidylserine (annexin‐V binding) and activation of the GPIIb/IIIa receptor (PAC‐1 binding) above the levels observed with the individual treatments. Aggregation responses (ADP and collagen) were enhanced in the cold‐UVC platelets compared to both RT groups, but this was primarily mediated by cold‐storage. Haemostatic function, as measured using TEG, was similar between the groups. Conclusion Cold‐storage of UVC‐treated platelets reduced PI‐induced acceleration of glycolytic metabolism. However, combining cold‐storage and UVC‐treatment resulted in additional phenotypic changes compared to each treatment individually. Further work is required to understand the impact of these changes in clinical efficacy.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The impact of refrigerated storage of UVC pathogen inactivated platelet concentrates on in vitro platelet quality parameters

et al. 2018

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“…This provides promising evidence to suggest that the CEMport may be useful for discerning heparin rebound following protamine administration after cardiac surgery. Other potential applications for the CEMport include monitoring treatments for hemophilia to aid in drug dosing, differentiating clot quality in stored blood products, 47 potentially diagnosing cardiovascular diseases such as CAD before symptomatic onset, 1 and providing hemostasis therapy guidance in traumatic coagulopathies. 9…”

Section: Discussionmentioning

confidence: 99%

A portable blood plasma clot micro-elastometry device based on resonant acoustic spectroscopy

Krebs

Oldenburg

2015

Review of Scientific Instruments

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Abnormal blood clot stiffness is an important indicator of coagulation disorders arising from a variety of cardiovascular diseases and drug treatments. Here, we present a portable instrument for elastometry of microliter volume blood samples based upon the principle of resonant acoustic spectroscopy, where a sample of well-defined dimensions exhibits a fundamental longitudinal resonance mode proportional to the square root of the Young's modulus. In contrast to commercial thromboelastography, the resonant acoustic method offers improved repeatability and accuracy due to the high signal-to-noise ratio of the resonant vibration. We review the measurement principles and the design of a magnetically actuated microbead force transducer applying between 23 pN and 6.7 nN, providing a wide dynamic range of elastic moduli (3 Pa-27 kPa) appropriate for measurement of clot elastic modulus (CEM). An automated and portable device, the CEMport, is introduced and implemented using a 2 nm resolution displacement sensor with demonstrated accuracy and precision of 3% and 2%, respectively, of CEM in biogels. Importantly, the small strains (<0.13%) and low strain rates (<1/s) employed by the CEMport maintain a linear stress-to-strain relationship which provides a perturbative measurement of the Young's modulus. Measurements of blood plasma CEM versus heparin concentration show that CEMport is sensitive to heparin levels below 0.050 U/ml, which suggests future applications in sensing heparin levels of post-surgical cardiopulmonary bypass patients. The portability, high accuracy, and high precision of this device enable new clinical and animal studies for associating CEM with blood coagulation disorders, potentially leading to improved diagnostics and therapeutic monitoring.

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“…For the FOR, there is a panel of tests available including tests to monitor heparin treatment and for evaluation of the contribution of fibrin to the clot strength. The coagulation is activated via the extrinsic pathway or by addition of a specific PAR1-activating peptide in the TRAP test to activate platelets via thrombin receptor PAR1.The instrument has mainly been used for studies of the platelet contribution to whole blood coagulation and quality control of platelet concentrates [ 18 , 41 - 44 ]. The clinical studies published so far have been limited in number [ 45 - 48 ].…”

Section: Introductionmentioning

confidence: 99%

Assays of different aspects of haemostasis – what do they measure?

2015

Self Cite

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Haemostasis is a complex process affected by many factors including both cellular and plasma components. It is a multistep process starting with platelet adhesion to damaged endothelium and ending in clot fibrinolysis. There are several methods available to study different aspects of haemostasis including adhesion, aggregation, coagulation and fibrinolysis. This review describes the different methods, what aspects of haemostasis they measure and their limitations. Methods discussed include methods to study adhesion (e.g. PFA-100, cone and platelet(let) analyzer and perfusion chambers) and aggregation (e.g. Multiplate, VerifyNow and Plateletworks). Furthermore the principles behind viscoelastic haemostatic assays are presented as well as methods that can analyse aspects of haemostasis in plasma or platelet-rich-plasma samples (thrombin generation, overall haemostasis potential and Thrombodynamics Analyzer).

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In vitro properties of platelets stored in a small container for pediatric transfusion

Abstract: Storage of a low number of PLTs benefits by storage in a small container in terms of better maintained in vitro properties.

Cited by 3 publications

References 20 publications

The impact of refrigerated storage of UVC pathogen inactivated platelet concentrates on in vitro platelet quality parameters

The impact of refrigerated storage of UVC pathogen inactivated platelet concentrates on in vitro platelet quality parameters

A portable blood plasma clot micro-elastometry device based on resonant acoustic spectroscopy

Assays of different aspects of haemostasis – what do they measure?

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