In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

Zhou, Yuguang; Wang, Baikui; Wang, Qi; Tang, Li; Zou, Peng; Zeng, Zihan; Zhang, Huihua; Gong, Li; Li, Weifen

doi:10.21203/rs.3.rs-463175/v1

Cited by 2 publications

(1 citation statement)

References 44 publications

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“…Blocking the NOD1 receptor causes symptoms of stress-induced anxiety, cognitive impairment and depression [118]. Lactobacillus plantarum Lac16 increased NOD1 expression level [119]. In the current study, NOD1 expression level decreased in the S group, Bactolac was not effective in increasing NOD1 expression level in stressed rats, but NOD1 expression level was similar in the C and B groups.…”

Section: Evaluation Of the Behavioral Testscontrasting

confidence: 46%

Probiotic Bactolac Provides Antidepressant Effect by Increasing BDNF level and Reducing NLRP3 and MC4R Expression Levels, Neuron Damage, Glial Activity and Depression-Like Behaviors in Rats

Işık,

Köse,

Budak

et al. 2024

Preprint

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Aim:Chronic stress plays an important role in the pathophysiology of depression. In this study, we aimed to investigate the therapeutic effects of Bactolac (Lactobacillus plantarum NBIMCC 8767+Streptococcus thermophilus NBIMCC 8258) on chronic stress-induced depression in rats. Methods:This study was performed in a total of 28 rats, in control (C), stress (S), Bactolac (B), Bactolac+stress (BS) groups (each n=7). Behavioral tests were performed to evaluate depressive behaviors (sucrose preference test, three-chamber sociability test, elevated plus maze test, forced swim test). 5-HT1A, DRD1, ADRA-2A, GABA-A α1, CNR1, NR3C2, NLRP3, MC4R expression levels in hippocampus tissue; NOD1 receptor expression levels in small intestine tissue were determined by RT-PCR method. BDNF levels in hippocampus tissue were determined by elisa kit. Histopathological examinations were performed in cortex and small intestine tissues. Results:Bactolac treatment reduced depressive-like behaviors (p<0.05), NLRP3 and MC4R expression levels (p<0.05), neurodegeneration, and glial activity, increased BDNF levels. It had no effect on other parameters examined in stressed rats. Conclusions:Chronic stress decreased the expression levels of 5-HT1A, DRD1, ADRA-2A, GABA-A α1, CNR1, NR3C2, NOD1 and BDNF level; increased the expression levels of NLRP3 and MC4R, caused neurodegeneration and glial activity, ultimately led to depressive effects. Bactolac was effective in reducing depressive effects and may be a natural treatment agent for depression.

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Section: Evaluation Of the Behavioral Testscontrasting

confidence: 46%

Probiotic Bactolac Provides Antidepressant Effect by Increasing BDNF level and Reducing NLRP3 and MC4R Expression Levels, Neuron Damage, Glial Activity and Depression-Like Behaviors in Rats

Işık,

Köse,

Budak

et al. 2024

Preprint

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High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation

Yang

Fan

et al. 2023

Foods

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Probiotic supplementation is a key therapeutic strategy for promoting gut health and maintaining gut homeostasis by modulating functional microbiota. In this study, we isolated two lactic acid bacteria (LAB) strains, Pediococcus pentosaceus TCM-3 and Lacticaseibacillus paracasei TDM-2, from Qinghai-Tibetan plateau, and evaluated their probiotic properties and antioxidant bioactivity. In which, TDM-2 had higher T-AOC activity than either TCM-3 or LGG (4.10 μmol/mL vs. 3.68 and 3.53 μmol/mL, respectively, p < 0.05). These strains have shown high antioxidant activity compared to the LAB strains and were found to be acid and bile salt tolerant, confronting the safety issues of antibiotic resistance and the capability of surviving in simulated gastric and intestinal juices. In vitro fermentation experiments with human gut microbiota revealed significant differences in microbial community composition between samples supplemented with TCM-3 and TDM-2 and those without. The addition of these two strains resulted in an enrichment of beneficial taxa, such as the Pediococcus, Lactobacillus, and Clostridium_sensu_strictos at the genus level, and Firmicutes and Proteobacteria at the phylum level. Notably, the TCM-3 group exhibited higher short-chain fatty acid production than the TDM-2 group and untreated controls (acetic acid at 12 h: 4.54 mmol L−1 vs. 4.06 mmol L−1 and 4.00 mmol L−1; acetic acid at 24 h: 4.99 mmol L−1 vs. 4.90 mmol L−1 and 4.82 mmol L−1, p < 0.05). These findings demonstrate that LAB supplementation with high antioxidant capacity and probiotic properties can promote gut health by modulating functional microbiota and is enriching for beneficial taxa. Our study provides guidance for therapeutic strategies that use novel LAB strains to maintain gut homeostasis and functional microbiota modulation.

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In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

Cited by 2 publications

References 44 publications

Probiotic Bactolac Provides Antidepressant Effect by Increasing BDNF level and Reducing NLRP3 and MC4R Expression Levels, Neuron Damage, Glial Activity and Depression-Like Behaviors in Rats

Probiotic Bactolac Provides Antidepressant Effect by Increasing BDNF level and Reducing NLRP3 and MC4R Expression Levels, Neuron Damage, Glial Activity and Depression-Like Behaviors in Rats

High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation

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