In Vitro Reconstitution of Bacterial DMSP Biosynthesis

Liao, Cangsong; Seebeck, Florian P.

doi:10.1002/ange.201814662

Cited by 14 publications

(5 citation statements)

References 39 publications

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“…These are DsyB in some alphaproteobacteria (Curson et al, 2017), DSYB in algae and corals (Curson et al, 2018), and TpMMT in the diatom Thalassiosira pseudonana (Kageyama et al, 2018). Recently, the Met methyltransferase, MmtN of the Met methylation pathway was identified in some Gram-positive bacteria, alphaproteobacteria, and gamma-proteobacteria (Liao and Seebeck, 2019;Williams et al, 2019). Currently, no enzymes of the Met decarboxylation pathway are known.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Insights Into the Cycling of Dimethylsulfoniopropionate and Related Molecules in the Eastern China Marginal Seas

et al. 2020

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The microbial cycling of dimethylsulfoniopropionate (DMSP) and its gaseous catabolites dimethylsulfide (DMS) and methanethiol (MeSH) are important processes in the global sulfur cycle, marine microbial food webs, signaling pathways, atmospheric chemistry, and potentially climate regulation. Many functional genes have been identified and used to study the genetic potential of microbes to produce and catabolize these organosulfur compounds in different marine environments. Here, we sampled seawater, marine sediment and hydrothermal sediment, and polymetallic sulfide in the eastern Chinese marginal seas and analyzed their microbial communities for the genetic potential to cycle DMSP, DMS, and MeSH using metagenomics. DMSP was abundant in all sediment samples, but was fivefold less prominent in those from hydrothermal samples. Indeed, Yellow Sea (YS) sediment samples had DMSP concentrations two orders of magnitude higher than in surface water samples. Bacterial genetic potential to synthesize DMSP (mainly in Rhodobacteraceae bacteria) was far higher than for phytoplankton in all samples, but particularly in the sediment where no algal DMSP synthesis genes were detected. Thus, we propose bacteria as important DMSP producers in these marine sediments. DMSP catabolic pathways mediated by the DMSP lyase DddP (prominent in Pseudomonas and Mesorhizobium bacteria) and DMSP demethylase DmdA enzymes (prominent in Rhodobacteraceae bacteria) and MddA-mediated MeSH S-methylation were very abundant in Bohai Sea and Yellow Sea sediments (BYSS) samples. In contrast, the genetic potential for DMSP degradation was very low in the hydrothermal sediment samples-dddP was the only catabolic gene detected and in only one sample. However, the potential for DMS production from MeSH (mddA) and DMS oxidation (dmoA and ddhA) was relatively abundant. This metagenomics study does not provide conclusive evidence for DMSP cycling; however, it does highlight the potential importance of bacteria in the synthesis and catabolism of DMSP and related compounds in diverse sediment environments.

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Section: Introductionmentioning

confidence: 99%

Metagenomic Insights Into the Cycling of Dimethylsulfoniopropionate and Related Molecules in the Eastern China Marginal Seas

et al. 2020

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“…The homologous dsyB (in bacteria) and DSYB (in algae) genes, and TpMMT in the diatom Thalassiosira pseudonana encode the key methylthiohydroxybutyrate methyltransferase enzyme of the transamination pathway [6][7][8] . In bacteria producing DMSP via the Met methylation pathway, mmtN encodes the key Met methyltransferase enzyme 9,10 . Recent studies suggest bacteria are likely important DMSP producers in coastal sediments, which have far higher DMSP-standing stocks than surface seawater samples where phytoplankton likely drive DMSP production 7,10 .…”

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confidence: 99%

Bacteria are important dimethylsulfoniopropionate producers in marine aphotic and high-pressure environments

et al. 2020

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Dimethylsulfoniopropionate (DMSP) is an important marine osmolyte. Aphotic environments are only recently being considered as potential contributors to global DMSP production. Here, our Mariana Trench study reveals a typical seawater DMSP/dimethylsulfide (DMS) profile, with highest concentrations in the euphotic zone and decreased but consistent levels below. The genetic potential for bacterial DMSP synthesis via the dsyB gene and its transcription is greater in the deep ocean, and is highest in the sediment.s DMSP catabolic potential is present throughout the trench waters, but is less prominent below 8000 m, perhaps indicating a preference to store DMSP in the deep for stress protection. Deep ocean bacterial isolates show enhanced DMSP production under increased hydrostatic pressure. Furthermore, bacterial dsyB mutants are less tolerant of deep ocean pressures than wild-type strains. Thus, we propose a physiological function for DMSP in hydrostatic pressure protection, and that bacteria are key DMSP producers in deep seawater and sediment.

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“…[7,8] DMSP belongs with an estimated annual production by marine organisms in the petagram range to the most abundant sulfur metabolites on earth [9] and is present in many marine and estuarine organisms including green algae, [10] dinoflagellates, [11] coccolithophores, [3] higher plants, [12] and corals. [13] The biosynthetic pathways toward DMSP have been investigated in green algae, [14] plants, [15][16][17] corals [18] and bacteria, [19,20] revealing differences for DMSP biosynthesis in these organisms, but all pathways start from l-methionine and proceed in different orders of steps through S-methylation, decarboxylation, and deamination with oxidation.…”

Section: Introductionmentioning

confidence: 99%

On the Substrate Scope of Dimethylsulfonium Propionate Lyases toward Dimethylsulfoxonium Propionate Derivatives

Chhalodia,

Dickschat

2024

Eur J Org Chem

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The six dimethylsulfonium propionate (DMSP) lyases DddQ, DddW, DddP, DddY, DddK and DddL catalyze the elimination of dimethyl sulfide from DMSP and can also cleave the marine metabolite dimethylsulfoxonium propionate (DMSOP) to DMSO and acrylate. In this study the potency of all six enzymes for the conversion of four DMSOP analogs with longer alkyl chains that were synthesized in three steps from 3‐mercaptopropionic acid was investigated. For this purpose, the pH dependency of the enzyme reactions was determined, showing optimal conditions at pH 8 for all enzymes but DddP, for which an optimum of pH 6 was found. Efficient transformations were observed for DddQ, DddW, DddY and DddK, for which the Michaelis‐Menten kinetics were determined for all four substrate analogs. HPLC analysis of the dialkylsulfoxides obtained with the most efficient enzyme DddW revealed that these compounds were obtained with a low to moderate enantiomeric excess (up to 25% ee), demonstrating that this enzyme has a preference for one of the enantiomers of the DMSOP analogs.

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In Vitro Reconstitution of Bacterial DMSP Biosynthesis

Cited by 14 publications

References 39 publications

Metagenomic Insights Into the Cycling of Dimethylsulfoniopropionate and Related Molecules in the Eastern China Marginal Seas

Metagenomic Insights Into the Cycling of Dimethylsulfoniopropionate and Related Molecules in the Eastern China Marginal Seas

Bacteria are important dimethylsulfoniopropionate producers in marine aphotic and high-pressure environments

On the Substrate Scope of Dimethylsulfonium Propionate Lyases toward Dimethylsulfoxonium Propionate Derivatives

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