In Vitro Regeneration and Antioxidant Propertiesof Lycium Barbarum L. (Goji)

Abstract

Ethnobotanical studies has documented the consumption of goji by Chinese tribes since ancient times. Goji or scientifically known as Lycium barbarum L. belongs to Solanaceae family and native to some areas of China. The present study was conducted to determine the best explant and corresponding hormonal compositions for Goji in vitro regeneration. In addition, the age and organ of in vitro seedlings with the optimum level of antioxidant activity were also identified. For in vitro regeneration, leaves and nodes were used as explants and cultured on in vitro regeneration media with varying hormonal concentration, combinations of α-napthaleneacetic acid (NAA) and 6-benzyl amino purine (BAP). The optimum combination for in vitro regeneration was found in leaf explants treated with equal concentration of 0.5 mg/L NAA and BAP each. DPPH assay for antioxidant activity screening done on methanolic extracts of samples from two through five month-old in vitro seedlings.shows the highest antioxidant activity in two-month old leaf and stem samples with the EC50 value of 0.08 mg/ml. Indeed, the results revealed that L. barbarum has the potency to be excellently micropropagated that possesses an outstanding antioxidant activity that is essential in phytomedicines.

Biologically Active Compounds from Goji (Lycium Barbarum L.) Leaves Aqueous Extracts: Purification and Concentration by Membrane Processes

“…In addition, Goji leaves have been described as a sustainable source of antioxidant compounds [ 9 , 10 ]. The biological properties have been related to complementary, additive, or synergistic interactions between the high content of vitamins, minerals (mainly calcium, iron, and zinc), and a diversity of polyphenols, alkaloids, and polysaccharides [ 11 , 12 ].…”

Large-Scale Plant Production of Lycium barbarum L. by Liquid Culture in Temporary Immersion System and Possible Application to the Synthesis of Bioactive Substance

“…Some authors reported the use of BAP in combination with α-napthaleneacetic acid (NAA) to induce direct or indirect organogenesis, and indirect somatic embryogenesis of goji starting from node or leaf explants. One example is the study of Osman et al [21], where the best result of plantlet regeneration through indirect organogenesis was obtained combining BAP and NAA (both at 0.5 mg L −1 ) starting from leaf explants. Leaf explants were also identified as the better way to induce indirect somatic embryogenesis on Murashige and Skoog (MS) medium [22], added of 1.0 mg L −1 2,4-dichlorophenoxyacetic acid and 0.1 mg L −1 BAP [23].…”

“…In this context, tissue culture is a valuable technique to produce secondary metabolites, and many studies showed the improvements that can be obtained in many species such as, in the same Lamiaceae family, sage [3] and oregano [34]. The advantage to produce antioxidants by goji in vitro culture was also underlined by Osman et al [21] and Dutu et al [35] on 2-month old plantlets, using the conventional micropropagation. In particular, the latter Authors tested the influence of different lengths wave (white; blue 473 nm; green 533 nm; yellow 580 nm; red 680 nm) on TPC and TFC content of goji leaves.…”

Health-Promoting Capacities of In Vitro and Cultivated Goji (Lycium chinense Mill.) Fruit and Leaves; Polyphenols, Antimicrobial Activity, Macro- and Microelements and Heavy Metals

“…Goji shrubs are propagated vegetatively, especially by semi-wooden cuttings and with great success in vitro [ 9 ]. According to Dzhugalov et al [ 10 ], the optimum quality fruit can be obtained under hot summer conditions.…”