In vitro regeneration and cardenolide determination of an endemic foxglove, Digitalis cariensis (Aegean Foxglove)

Mohammed, Aliyu; Yücesan, Buhara; Demir-Ordu, Öznur; Cihangir, Cansu; Eker, İsmail; Kreis, Wolfgang; Gürel, Ekrem

doi:10.1007/s11627-015-9697-6

Cited by 10 publications

(9 citation statements)

References 39 publications

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“…The success of TDZ in regenerating shoots from the calli of A. erectifolius might be because TDZ has both auxin and cytokinin activities, unlike natural cytokinins (Mohammed et al, 2015). This is in accordance with a report by Guo et al (2011), which stated that TDZ has auxin-and cytokinin-like effects in different plant species.…”

Section: Basesupporting

confidence: 88%

“…Selection of the best explants for callus induction and shoot regeneration has been the most critical factor in the micropropagation of many plant species (Sripaoraya et al, 2003;Mohammed et al, 2015;Takamori et al, 2015;Yücesan et al, 2015). Therefore, to select the best explant for indirect organogenesis in A. erectifolius, different types of explants were tested.…”

Section: Effect Of Explant Sourcementioning

confidence: 99%

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Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species

Moreira¹,

Andrade²,

Bertolucci³

et al. 2016

Turk J Biol

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The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3-or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N 6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.

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Section: Basesupporting

confidence: 88%

Section: Effect Of Explant Sourcementioning

confidence: 99%

Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species

Moreira¹,

Andrade²,

Bertolucci³

et al. 2016

Turk J Biol

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“…D. cariensis seeds germinate poorly after extensive dormancy. Seed germination was improved when seeds were pretreated with scarification followed by soaking in sterile distilled water overnight [89].…”

Section: Agriculturementioning

confidence: 99%

The Foxgloves (Digitalis) Revisited

Kreis

2017

Planta Med

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This review provides a renewed look at the genus Digitalis. Emphasis will be put on those issues that attracted the most attention or even went through paradigmatic changes since the turn of the millennium. PubMed and Google Scholar were used (“Digitalis” and “Foxglove” were the key words) to identify research from 2000 till 2017 containing data relevant enough to be presented here. Intriguing new results emerged from studies related to the phylogeny and taxonomy of the genus as well as to the biosynthesis and potential medicinal uses of the key active compounds, the cardiac glycosides. Several Eastern and Western Foxgloves were studied with respect to their propagation in vitro. In this context, molecular biology tools were applied and phytochemical analyses were conducted. Structure elucidation and analytical methods, which have experienced less exciting progress, will not be considered here in great detail.

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“…Organogenesis had been achieved in different species of Digitalis genus e.g. D. thapsi (Cacho et al, 1991), D. trojana (Çördük and Aki, 2010), D. davisiana (Gurel et al, 2011), D. lamarkii (Verma et al, 2011a,b), D. nervosa (Karimi and Kazemitabar, 2013), D. purpurea (Patil et al, 2013;Li et al, 2014) and recently in D. cariensis (Mohammed et al, 2015). The success of regeneration depends on the type and concentration of plant growth regulator.…”

Section: In Vitro Morphogenesis Of Digitalis Purpureamentioning

confidence: 99%

“…As a consequence, several research groups have developed biotechnological strategies in order to reduce the excessive use of natural Digitalis populations, to conserve high yielding cardenolide producing plants or for genetic improvement. Such strategies include somatic embryogenesis (Lindemann and Luckner, 1997), temporary immersion systems (Pérez-Alonso et al, 2009;), precursors addition, elicitation (Pérez-Alonso et al, 2014aPatil et al, 2013) and organogenesis (Hagimori et al, 1982;Pérez-Bermúdez et al, 1984;Cacho et al, 1991;Fatima et al, 2009;Çördük and Aki, 2010;Gurel et al, 2011;Verma et al, 2011a,b;Karimi and Kazemitabar, 2013;Li et al, 2014;Pérez-Alonso et al, 2014b;Yücesan et al, 2014;Kreis et al, 2015;Mohammed et al, 2015). Organogenesis can be done directly or indirectly, direct organogenesis being the most successful for many species of the genus Digitalis.…”

Section: Introductionmentioning

confidence: 99%

Efﬁcient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

Pérez-Alonso

Martín

Capote

et al. 2018

Industrial Crops and Products

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In vitro regeneration and cardenolide determination of an endemic foxglove, Digitalis cariensis (Aegean Foxglove)

Cited by 10 publications

References 39 publications

Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species

Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species

The Foxgloves (Digitalis) Revisited

Efﬁcient direct shoot organogenesis, genetic stability and secondary metabolite production of micropropagated Digitalis purpurea L.

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