In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak, Pradipta K.; Kalidass, Chinnamadasamy

doi:10.3329/ptcb.v26i2.30575

Cited by 7 publications

(8 citation statements)

References 27 publications

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“…The number of roots and its length decreased with 1.0 mg.L -1 IBA supplemented media. Earlier, Nayak & Kalidass (2016) reported similar observations in Blepharispermum subsessile.…”

Section: Discussionsupporting

confidence: 70%

In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

Bindu¹,

Dharmapal²,

Udayan³

et al. 2017

Trop. Plant Res.

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Pueraria tuberosa, commonly known as Indian kudzu or 'Vidari' is an important medicinal plant belonging to the family Fabaceae. The tubers of this plant are an important constituent in many Ayurvedic formulations as a restorative tonic, immune booster and antiageing. Annual demand of Vidari by the Ayurvedic industry is 135 tonnes and industry is facing a severe scarcity of this raw material. Micropropagation technology offers large-scale production of disease-free, quality planting materials for pharmaceutical industries. A successful protocol for the in vitro propagation of P. tuberosa has been achieved by using nodal segments as explants. Multiple shoot induction and proliferation was obtained in Murashige and Skoog medium supplemented with 0.5 mg.L -1 BAP, 0.5 mg.L -1 KN and 2% glucose. MS medium with 1.5 mg.L -1 KN favoured maximum shoot elongation. A maximum number of roots with the highest percentage of rooting was observed on half strength MS media supplemented with 0.5 mg.L -1 IBA. Elongated shoots in half strength basal MS medium induced roots in 14 days of culture. Regenerated shoots with well-developed roots were successfully hardened with 80% survival.

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“…The number of roots and its length decreased with 1.0 mg.L -1 IBA supplemented media. Earlier, Nayak & Kalidass (2016) reported similar observations in Blepharispermum subsessile.…”

Section: Discussionsupporting

confidence: 70%

In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

Bindu¹,

Dharmapal²,

Udayan³

et al. 2017

Trop. Plant Res.

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“…During this experiment, a clear difference in percentage of seed germination and mean number of shoots per explants was observed than our previous work. [1] In our earlier work, [1] the seeds used for the study were the ones freshly collected. They were used immediately without any drying or storage period.…”

Section: Discussionmentioning

confidence: 99%

“…It is found in the moist deciduous forest areas of Odisha, Karnataka, Madhya Pradesh, and Maharastra of India. The species is used in Indian System of Medicine for treatment of various ailments and also by tribals for various diseases . Its aromatic rhizomes are used in havan samagri .…”

Section: Introductionmentioning

confidence: 99%

“…The species is used in Indian System of Medicine for treatment of various ailments and also by tribals for various diseases. [1] Its aromatic rhizomes are used in havan samagri. [2] Several chromenes were isolated from the aerial parts and rhizomes of B. subsessile [3][4][5] ; these include desmethyl isoencecalin, 5-hydroxy-6-acetyl-2-hydroxy methyl-2-methyl chromene.…”

Section: Introductionmentioning

confidence: 99%

“…Effect of different concentrations of IBA and IAA on induction of roots in in vitro raised shoots of Blepharispermum subsessile1,2 Mean values within column followed by the same letter in superscript are not significantly different (p ≤ .05; Duncan's multiple range test).…”

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confidence: 99%

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PIXE‐based detection of elemental accumulation during direct organogenesis in Blepharispermum subsessileDC.: An endangered medicinal plant of Odisha, India

2017

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Particle‐induced X‐ray emission was used to investigate mineral accumulation during different developmental stages of direct organogenesis from cotyledon explants of Blepharispermum subsessile beginning from shoot bud initiation and formation to in vitro regenerated roots. Mineral uptake and accumulation appeared selective and varied between different stages of shoot bud initiation and formation (Stage 1), proliferation of leafy shoots (Stage 2), and in vitro regenerated roots (Stage 3). The concentrations of 2 macro elements, K and Ca, were found in higher quantity during proliferation of shoot buds to leafy shoots stage suggesting their role in cell division, bud formation, and multiplication of the plant. Most of the micronutrients such as Mn, Fe, Ni, Cu, and Zn were found to be accumulated in higher quantities in in vitro regenerated roots, as they provide the plant with a larger surface area and hence a greater potential for mineral uptake. The results of particle‐induced X‐ray emission test suggest that the information on the accumulation of elements during developmental stages in vitro could be useful for formulating a media for the induction of high‐frequency regeneration of in this important endangered medicinal plant species for its ex situ conservation.

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