In vitro regeneration of evergreen azalea from leaves

Mertens, M.; Werbrouck, Stefaan; Samyn, G.; Silva, H. Botelho dos Santos Moreira da; Debergh, Pierre

doi:10.1007/bf00043635

Cited by 22 publications

(10 citation statements)

References 11 publications

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“…However, in Rhododendron spp., the frequency of plant regeneration from leaf explants is very different and the efficiency of growth regulators is cultivar specific (e.g. Preece and Imel 1991, Mertens et al 1996, Hsia and Korban 1998b, Iapichino et al 1991, Tomsone and Gertnere 2003. We previously showed that transformation of explants with meristematic tissues produced many problems such as the appearance of chimeric plants with gradual loss of transgenes during vegetative propagation (Pavingerová et al 1997, Pavingerová andŠedivá 1999).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

Pavingerová¹

2009

Biologia plant.

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The influence of cytokinin thidiazuron (TDZ) and auxin indole-3-acetic acid (IAA) on in vitro shoot organogenesis of fifteen Rhododendron genotypes was investigated and a protocol for high frequency adventitious shoot regeneration from leaf explants was developed. High genotypic variation was observed and regeneration frequencies ranged from 0 to 100 %. Genotype Ovation had the highest number of shoots (26.4 per explant) after 12 weeks on medium with 0.57 μM IAA and 1.20 μM TDZ, but only 65 % of explants regenerated. Catawbiense Grandiflorum had 17.7 shoots per explant and 75 % regeneration on medium with 5.70 μM IAA and 0.45 μM TDZ and Van Werden Poelman had 14.3 shoots per explant and 100 % regeneration on medium with 0 57 μM IAA and 0.45 μM TDZ.

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

Pavingerová¹

2009

Biologia plant.

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“…Nakai (Yodogawa azalea), R. 'Boule de Neige', and R. 'Gibraltar' (McCown and Lloyd, 1982); R. ponticum L. (pontic rhododendron) (Almeida et al, 2005); R. simsii Planch. 'Helmut Vogel' (Mertens, 1996); R. catawbiense Michx. 'English Roseum' (Sicuranza and Mitkowski, 2007); and R. P.J.M.…”

mentioning

confidence: 99%

“…is most commonly stimulated by a combination of the cytokinins, 6-(g,g-dimethylallylamino) purine (2iP) or zeatin, and auxins, indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). To maximize shoot regeneration from leaves, 2iP and zeatin are typically required in high concentrations often exceeding 50 mM (Iapichino and Chen, 1995;Iapichino et al, 1992;Mertens, 1996;Tomsone and Gertnere, 2003). In comparison, thidiazuron [6-(g,g-dimethylallylamino)] (TDZ) has been an effective cytokinin in some species and can be used in lower concentrations, often making it more efficient (Bates et al, 1992;Huetteman and Preece, 1993).…”

mentioning

confidence: 99%

In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron ‘Fragrantissimum Improved’

Hebert¹,

Touchell²,

Ranney³

et al. 2010

horts

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Rhododendron L.‘Fragrantissimum Improved’ is an attractive cultivar with showy, fragrant flowers but has limited potential for breeding because it is a sterile wide hybrid. Protocols for in vitro regeneration and polyploid induction were developed for this cultivar as a means to potentially restore fertility and enhance ornamental traits. Combinations of thidiazuron (TDZ) at 0, 5, 10, 15, or 20 μM and 1-naphthaleneacetic acid (NAA) at 0, 2.5, 5, or 10 μM were used to induce shoot regeneration from leaves. Shoot regeneration was optimized (68% of leaf segments produced shoots) using 8.8 μM TDZ and 10 μM NAA. To induce polyploidy, regenerative callus was treated with 7.5, 15, 30, 60, or 90 μM of the mitotic inhibitor oryzalin for 1, 3, 5, 7, or 14 d in various combinations. Oryzalin significantly affected survival and shoot regenerative capacity. A percentage of homogenous, tetraploid shoots was recovered from treatments of 30 μM oryzalin for 1 (13%) or 3 (13%) days and 7.5 μM oryzalin for 7 (20%) or 14 (7%) days.

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“…Moreover some researchers 23,24 have reported TDZ to be an essential growth regulator for shoot induction from leaf explant of Ericaceae family.…”

Section: Resultsmentioning

confidence: 99%

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Ranyaphia¹,

Maoa²,

Borthakurb³

2011

ScienceAsia

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Protocol for rapid regeneration of the important medicinal plant Gaultheria fragrantissima Wall. (Ericaceae) through direct organogenesis from leaf, internode (stem without axillary bud), and root explants of in vitro raised seedlings has been developed. Different cytokinins (thidiazuron, 6-benzyladenine, kinetin, and 2-isopentenyl adenine) at a range of various concentrations in modified rhododendron medium have been investigated for organogenesis. Among the four cytokinin investigated, only thidiazuron (TDZ) was effective for direct shoot regeneration from leaf and internode explants but root explants failed to respond. Percentage of response in shoot bud induction increases as the concentration of TDZ increases. After 6 weeks culture on TDZ supplemented medium the shoots were elongated on medium devoid of TDZ. Although the percentage of response was larger at higher concentration of TDZ, 1-2 mg/l TDZ was the optimum concentration for obtaining normal, healthy shoots. The shoots developed from shoot bud induced at higher concentration of TDZ were stunted and hyperhydric. Explant culture on induction medium containing TDZ for more than 6 weeks has a detrimental effect on shoot development. Supplement of 2iP (5, 8 mg/l) or Kin (4, 6 mg/l) on the development medium enhanced the number of shoot and shoot length. Half strength modified rhododendron medium supplemented with 2iP (5 mg/l) was the ideal shoot development medium, producing 12-16 shoots from single explant within 8 weeks. Significant carry over effect of TDZ on root inhibition of microshoots was observed. Two month old rooted plantlets were transferred from lab to land and successfully established.

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In vitro regeneration of evergreen azalea from leaves

Cited by 22 publications

References 11 publications

The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

The influence of thidiazuron on shoot regeneration from leaf explants of fifteen cultivars of Rhododendron

In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron ‘Fragrantissimum Improved’

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