In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

Saha, Priya; Afrin, M; Mohiuddin, A. K. M.; Shohael, Abdullah Mohammad

doi:10.3329/jujbs.v4i2.27790

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…Sucrose was shown to play a vital role in improving plant growth and shoot regeneration rate, while glucose and maltose played a minor role in shoot induction. The findings in the current study are consistent with previous studies which reported that sucrose induced shoot formation of Soybean [66], Velvet bean [67], Peach [68], Gotu Kola [16], Pea [69,70], Kris [71], Cucumber [72]. The types of carbohydrate sources, and their concentrations may influence culturing success [63,65].…”

Section: Discussionsupporting

confidence: 92%

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Aslam

Karanja

Zhang

et al. 2020

Plants

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The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.

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Section: Discussionsupporting

confidence: 92%

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Aslam

Karanja

Zhang

et al. 2020

Plants

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“…A lo anterior, se puede sumar que el TDZ estimula la síntesis de auxinas endógenas (Murthy et al 1995, De Melo et al 2006) y se ha reportado que un aumento de las concentraciones de 3-ácido indolacético (IAA) en los sitios celulares induce anormalidades en el crecimiento, como inhibición de los brotes (disminución de la elongación y el área foliar) (Saha et al 2015).…”

Section: Resultados Y Discusiónunclassified

Efecto del genotipo y fuente de citoquinina en la etapa de iniciación de cultivo in vitro de tejido adulto de Castanea sativa Mill.

Larson¹,

Hasbún²,

Jofré³

et al. 2017

Gayana Bot.

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“…NLK-40 exhibited 92% response to callusing and it took 30-32 days to produce full grown green compact callus. Afterwards, Saha et al, (2015) highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used.…”

Section: Organogenesismentioning

confidence: 97%

“…The best rooting was achieved on MS medium containing 0.90 mg/l NAA. Saha et al, 2015). Tripathy et al, (2013) internode explants of grass pea (Lathyrus sativus L.) pre-cultured in B5 with 2,4-D (2 mg/l) + BAP (0.5 mg/l) when transferred to BM medium containing 0.5 mg/l NAA+2 mg/l BAP; produced conspicuous, glossy, dark green and well developed somatic embryos.…”

Section: Somatic Embriyogenesismentioning

confidence: 99%

In vitro regeneration techniques in the grass pea (Lathyrus sativus L.) plant

Çatal

Bakoğlu

2018

Eurasian Journal of Forest Science

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The Lathyrus genus is in the legumes family with annual or perennial species number of 160. There are 58 species naturally grown in Turkey, 18 of them are endemic. In Rize province, 8 species were identified, 2 of them were endemic. In the world, Lathyrus species are evaluated in the animal feed as green grass, hay and grain feed, fertilization of soil as a green manure plant and human nutrition as food grain legume plant. The cultivation of Lathyrus species are very rare in our country and are generally used in animal feeding and in small quantities in human nutrition. Grass pea (Lathyrus sativus L.) plant is the most used species in the world, because it is resistant to adverse soil conditions, drought and flooding. Various tissue culture methods are used to develop and reproduce this species. Plant tissue culture is being applied both in the development of new varieties and genetic changes in existing varieties, and in the production of species which are difficult to reproduce and protect of the disappearing species. The basic system used in plant tissue culture processes and genetic improvements is plant regeneration. In this review, some studies related to the in vitro regeneration techniques of the grass pea plants have been put together and the techniques used in regeneration have been evaluated.

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In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

Cited by 6 publications

References 19 publications

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Efecto del genotipo y fuente de citoquinina en la etapa de iniciación de cultivo in vitro de tejido adulto de Castanea sativa Mill.

In vitro regeneration techniques in the grass pea (Lathyrus sativus L.) plant

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