In vitro regeneration of multiple shoots from encapsulated somatic embryos of Artemisia vulgaris L.

Sudarshana, M. S.; Rajashekar, N.; Niranjan, M. H.; Borzabad, Rezwan Karami

doi:10.9790/3008-0661115

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…encapsulated calli obtained from a shoot tip of garlic, Allium sativum L., using a calcium alginate gel and stored for 40 days at 4 º C and 88% of the encapsulated calli regenerated after 40 days of storage at 4 º C. Somatic embryo was used in synthetic seeds in egglplant and highest germination rate was 81% on MS +1.0 mg/1 BAP + 0.1 mg/1 GA3 and these encapsulated embryos did not loose germination even after storage of 45 days at 4 º C (Huda and Bari 2007). Further, encapsulated somatic embryo retrieved from storage conditions was evaluated by Sudarshana et al (2013) for its viability. The encapsulated embryos could be stored up to 4 months at 4 º C, 20 º C and 22 º C. Maximum conversion frequency of 90% was observed from encapsulated somatic embryos cultured on MS medium supplemented with GA3 (1.5 mg/l), IAA (0.5 mg/l) and ascorbic acid (40 mg/l) and 22 º C temperature they found to be optimum irrespective of storage periods.…”

Section: Discussionmentioning

confidence: 99%

Development of artificial seed and preservation in Mimosa pudica L., an important medicinal plant in Bangladesh

Banu¹,

Rashid

Miah

2016

J bio-sci.

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Context: Mimosa pudica L. is an important medicinal plant belonging to the family-Mimosaceae has becoming a rare species in Bangladesh. The application of artificial seed technology using encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plant like Mimosa pudica L. Objective: Synthetic seed technology has been developed for Mimosa pudica L. in order to develop an alternative protocol on propagation and conservation. Materials and Methods: For this purpose shoot tip and nodal segments obtained from in vitro grown plants were encapsulated with sodium alginate solution followed by subsequent immersion in CaCl2 solution. Different concentrations and combinations of growth regulators were used and explants were treated in alginate bead to investigate the hormonal effect on artificial seed germination. These encapsulated seeds were cultured either on MS medium with hormone (same growth regulators containing alginate beads) or MS0 (without hormone). Results: Highest shoot regeneration frequency (100%) were recorded when alginate beads were infused by MS medium supplemented with 2.0 mg/l BAP + 0.2 mg/l NAA and cultured in MS medium containing same growth regulators. When synthetic seed containing 2.0 mg/l BAP+0.2 mg/l NAA and cultured on MS0 medium, 54% explants produced multifarious root with shoot in both cases. Under different storage period encapsulated seed retained germination capacity even after preserving for 60 days at 4 0 C. Conclusion: For artificial seed production a suitable protocol established under this study for Mimosa pudica L. that provides an alternative method for micropropagation and its conservation. For long term storage of Mimosa pudica in Bangladesh this protocol would provide promising avenues for the easy transference of propagules and its improvement.

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Section: Discussionmentioning

confidence: 99%

Development of artificial seed and preservation in Mimosa pudica L., an important medicinal plant in Bangladesh

Banu¹,

Rashid

Miah

2016

J bio-sci.

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“…It was proved that the encapsulated embryos of A. vulgaris could be stored at 4 • C, 20 • C, and 22 • C for 4 months. These embryos showed the highest (90%) efficiency of conversion into microplants at 22 • C on MS medium containing 1.5 mg/L GA 3 , 0.5 mg/L IAA, and 40 mg/L ascorbic acid [137].…”

Section: Biotechnological Research On Micropropagationmentioning

confidence: 99%

Significance of Artemisia Vulgaris L. (Common Mugwort) in the History of Medicine and Its Possible Contemporary Applications Substantiated by Phytochemical and Pharmacological Studies

et al. 2020

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Artemisia vulgaris L. (common mugwort) is a species with great importance in the history of medicine and was called the “mother of herbs” in the Middle Ages. It is a common herbaceous plant that exhibits high morphological and phytochemical variability depending on the location where it occurs. This species is well known almost all over the world. Its herb—Artemisiae vulgaris herba—is used as a raw material due to the presence of essential oil, flavonoids, and sesquiterpenoids lactones and their associated biological activities. The European Pharmacopoeia has listed this species as a potential homeopathic raw material. Moreover, this species has been used in traditional Chinese, Hindu, and European medicine to regulate the functioning of the gastrointestinal system and treat various gynecological diseases. The general aim of this review was to analyze the progress of phytochemical and pharmacological as well as professional scientific studies focusing on A. vulgaris. Thus far, numerous authors have confirmed the beneficial properties of A. vulgaris herb extracts, including their antioxidant, hepatoprotective, antispasmolytic, antinociceptive, estrogenic, cytotoxic, antibacterial, and antifungal effects. In addition, several works have reviewed the use of this species in the production of cosmetics and its role as a valuable spice in the food industry. Furthermore, biotechnological micropropagation of A. vulgaris has been analyzed.

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“…In many cases, it provides an opportunity to maintain true-to-type plant species and the propagation system can produce a large number of plants from a single clone with enhanced artemisinin contents. In vitro manipulation of different Artemisia species such as A. annua [ 20 , 21 , 22 , 23 ], A. sieberi [ 24 ], A. vulgaris [ 25 , 26 ], A. japonica [ 27 ], A. nilagirica var. nilagirica [ 28 ], A. absinthium [ 29 , 30 ], A. abrotanum [ 31 ], A. amygdalina [ 32 ], A. carvifolia [ 33 ], A. aucheri [ 34 ], A. scoparia [ 35 ], A. judaica [ 36 ], A. absinthium [ 37 ], A. chamaemelifolia [ 38 ] and A. pallens [ 39 , 40 ] has been attempted for various purposes.…”

Section: Production Of the Sesquiterpene Artemisinin In mentioning

confidence: 99%

Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists

Guerriero

Berni

Muñoz-Sánchez

et al. 2018

Genes

285

138

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Plants are sessile organisms and, in order to defend themselves against exogenous (a)biotic constraints, they synthesize an array of secondary metabolites which have important physiological and ecological effects. Plant secondary metabolites can be classified into four major classes: terpenoids, phenolic compounds, alkaloids and sulphur-containing compounds. These phytochemicals can be antimicrobial, act as attractants/repellents, or as deterrents against herbivores. The synthesis of such a rich variety of phytochemicals is also observed in undifferentiated plant cells under laboratory conditions and can be further induced with elicitors or by feeding precursors. In this review, we discuss the recent literature on the production of representatives of three plant secondary metabolite classes: artemisinin (a sesquiterpene), lignans (phenolic compounds) and caffeine (an alkaloid). Their respective production in well-known plants, i.e., Artemisia, Coffea arabica L., as well as neglected species, like the fibre-producing plant Urtica dioica L., will be surveyed. The production of artemisinin and caffeine in heterologous hosts will also be discussed. Additionally, metabolic engineering strategies to increase the bioactivity and stability of plant secondary metabolites will be surveyed, by focusing on glycosyltransferases (GTs). We end our review by proposing strategies to enhance the production of plant secondary metabolites in cell cultures by inducing cell wall modifications with chemicals/drugs, or with altered concentrations of the micronutrient boron and the quasi-essential element silicon.

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In vitro regeneration of multiple shoots from encapsulated somatic embryos of Artemisia vulgaris L.

Cited by 6 publications

References 18 publications

Development of artificial seed and preservation in Mimosa pudica L., an important medicinal plant in Bangladesh

Development of artificial seed and preservation in Mimosa pudica L., an important medicinal plant in Bangladesh

Significance of Artemisia Vulgaris L. (Common Mugwort) in the History of Medicine and Its Possible Contemporary Applications Substantiated by Phytochemical and Pharmacological Studies

Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists

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