In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures

Abstract

Methods were developed in the present investigation for cloning and large scale plant production of Passiflora foetida L. germplasm selected from the East-Coast region of South India. Nodal shoot segments were used as explants. The explants were dressed and surface sterilized with 0.1% (w/v) HgCl 2 . Multiple shoots were induced (6.13 ± 0.22 shoots per explant) by proliferation of nodal shoot meristems on Murashige and Skoog (MS) semi-solid medium + 2.0 mg l À1 6-benzylaminopurine (BAP). The shoots of P. foetida were further multiplied (16.45 ± 0.44 shoots per explant) on MS medium + 0.5 mg l À1 each of BAP and Kinetin (Kin). The in vitro generated shoots were rooted on half-strength MS medium containing 2.5 mg l À1 indole-3 butyric acid (IBA). By this method 67% shoots were rooted. About 97% shoots were rooted ex vitro (8.33 ± 0.29 roots per shoot) when the cut ends of the shoots were treated with 300 mg l À1 IBA for 5 min. The in vitro rooted plants were hardened and acclimatized in the greenhouse and successfully (100%) transplanted to the field. Ó 2015 Production and hosting by Elsevier B.V. on behalf

Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

“…The ideal auxin concentration to be applied exogenously depends on the species being studied. For Passiflora foetida microcuttings, for example, 97% rooting was obtained at a lower concentration (300 mg L -1 ) than the ideal one estimated for H. lupulus in the present study (Shekhawat et al, 2015).…”

Multiplicación in vitro y embriogénesis somática de Perezia pinnatifida (Asteraceae) planta medicinal andina

“…mostró 100% de desinfección y sobrevivencia de las yemas. La aplicación de este desinfectante también fue efectiva en yemas de P. coerulescens (Olivera et al 2010), rizomas de Curcuma longa (Islam et al 2004), segmentos nodales pilosos de Passiflora foetida (Shekhawat et al 2015) porque la carga microbiana dependería de la ubicación y tipo de tejido. Adicionalmente, a 10 min.…”

“…La adición de BAP incrementa la formación de brotes en diversas plantas medicinales (Aslam et al 2013, Shekhawat et al 2015, Elamvaluthi et al 2016; probablemente porque las citoquininas activan a las yemas axilares de los explantos desencadenando la proliferación de brotes (Aremu et al 2016). En concordancia, la adición de BAP en el cultivo in vitro de P. pinnatifida fue esencial para la multiplicación de brotes, al igual que en Chlorophytum borivilianum (Shekhawat et al 2015).…”

“…Las herramientas biotecnológicas que involucran el cultivo in vitro son de gran importancia como técnicas de conservación ex situ (Barnicoat et al 2011) porque pueden ser aplicadas para conseguir una propagación rápida y masiva de especies de plantas de interés, como aquellas en riesgo de extinción, endémicas, raras, medicinales, entre otras (Shekhawat et al 2015); así mismo provee ADN para estudios moleculares, plántulas para estudios ecológi-cos y material para usos económicos (Sarasan et al 2006). Entre estas técnicas se encuentran la micropropagación, embriogénesis somática, organogénesis y criopreservación (Khan et al 2012).…”

In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction

“…Ex vitro rooting facilitates cost reduction by avoiding in vitro rooting, reducing labour and time of establishment from laboratory to field (Shekhawat et al 2015). In the study, pre treatment with IBA 1000 mg L .…”