In vitro Regeneration of the Medicinal Herb of Nilgiri shola, Acmella calva L. from Leaf Derived Callus

Senthilkumar, P.; Paulsamy, S.; Vijayakumar, K. K.; Kalimuthu, K.

doi:10.3329/ptcb.v17i2.2566

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…Earlier reports on in vitro regeneration of Spilanthes were carried out through direct or indirect organogenesis in Spilanthes mauritiana (Bais et al 2002) and Spilanthes acmella (Saritha et al 2002;Haw and Keng 2003;Saritha and Naidu 2008;Pandey and Agrawal 2009;Singh et al 2009a, b;Singh and Chaturvedi 2010). Though few reports (Senthilkumar et al 2007;Amudha and Shanthi 2011;Devi et al 2012) are available on regeneration of S. calva, they pertain to organogenesis through node or leaf derived calluses. Recently, Devi et al 2012 have published paper on micropropagation through encapsulation of nodal explants which were derived from in vitro shoots.…”

mentioning

confidence: 99%

In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker

Razaq

Heikrujam

Chetri

et al. 2012

Physiol Mol Biol Plants

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An efficient in vitro protocol has been established for clonal propagation of elite plant of Spilanthes calva DC., an important source of spilanthol, an antimalarial larvicidal compound. Nodal explants excised from field grown plant of S. calva DC. when reared on Murashige and Skoog's medium augmented with different cytokinins, viz. N 6 -Benzyladenine (BA), N 6 -(2-isopentenyl) adenine (2iP) and 6-furfuryl aminopurine (Kn), differentiated multiple shoots from the axils. BA at 10 μM proved optimum for elicitation of multiple shoots in 91.6 % cultures with an average of 7.12 shoots per explant within 6 weeks. The excised shoots rooted on half strength Murashige and Skoog's medium supplemented with 0.1 μM IBA. Micropropagated plants were hardened and transferred to field for acclimatization, where 95 % plants survived and were phenotypically similar to the donor plant. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were employed to evaluate the genetic fidelity amongst the regenerants. Eleven individuals, randomly chosen amongst a population of 120 regenerants were compared with the donor plant. A total of 71 scorable bands, ranging in size from 100 bp to 1,100 bp were generated by a combination of the two markers in the aforesaid plants. All banding profiles from micropropagated plants were monomorphic and similar to those of mother plant. The similarity values amongst the aforesaid plants varied from 0.967 to 1.000. The dendrogram generated through UPGMA (Unweighted Pair Group Method with arithmetic mean) analysis revealed 98 % similarity amongst them, thus confirming the genetic fidelity of the in vitro clones.

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mentioning

confidence: 99%

In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker

Razaq

Heikrujam

Chetri

et al. 2012

Physiol Mol Biol Plants

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“…In Germany, the plant extract is used for the treatment of soreness or bruising (7). Traditionally flower of Spilanthes paniculata are used in relieve toothache (8), in stomatitis and throat complaints (8), cure paralysis of tongue (9), leaves are used for remedy of stammering (9), as spice (10); local anaesthetic (11), in bacterial and fungal skin diseases (12) and roots are used in cold and flu (5), in headache, asthma and rheumatism (13).…”

Section: Received 3 March 2013/accepted 5 May 2013mentioning

confidence: 99%

Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

et al. 2015

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Bangladesh possesses a rich flora of medicinal plant. Out of the estimated 5000 species of phanerogams and pteridophytes growing in this country, more than a thousand are regarded as having medicinal properties. The aim of this study was to evaluate the in vitro antimicrobial activity and brine shrimp lethality of the whole plant extracts of Spilanthes paniculata, a plant belonging to the family Asteraceae. The crude extract, n-Hexane, chloroform and ethyl acetate soluble fraction of crude extract showed significant to moderate antimicrobial activity against three Gram positive and three Gram negative microorganisms. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, choloform and ethyl acetate soluble fractions were found to be 14.89 mm-19.40 mm, 14 mm-19.40 mm, 10.66 mm-13.50 mm and 9.50 mm-13.26 mm, consecutively at a concentration of 30 µg/disc. N-Hexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Spilanthes paniculata were screened for antitumor properties using brine shrimp lethality bioassay. From the results of the brine shrimp lethality bioassay, it can be well predicted that n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract possess mild cytotoxicity on shrimp naupalii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 48.978 mg/ml, 92.61 mg/ml and 216.770 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22743 Stamford Journal of Microbiology, Vol.3(1) 2013: 1-5

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“…Shoots regeneration via a callus phase was the simplest way to induce somaclonal variation and thus pave the way for improvement of the species (Thorpe et al, 1991). Such types of plant regeneration was also reported in many medicinal plant species including Abrus precatorius (Biswas et al, 2007), Acmella calva (Senthilkumar et al, 2007), Phellodendron amurense (Azad et al, 2005), Plumbago zeylanica (Das and Rout, 2002) and Holosma ada-kodien (Martin, 2002). Callus derived shoots were isolated ( Fig.…”

Section: Shoot Regenerationmentioning

confidence: 99%

Studies on <i>In vitro</i> Callus Induction and Regeneration from Nodal Explants of <i>Cassia alata</i> L.

Hasan

Hossain

et al. 2013

Progress. Agric.

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A successful protocol for adventitious shoot regeneration was developed from the nodal explant derived callus tissue of Cassia alata. Greenish friable callus was induced from the cut surface of the nodal explants on MS medium supplemented with 1.5 mgl -1 2,4-D within twenty days of inoculation. The calli differentiated into adventitious shoots when they were cultured on MS medium supplemented with 1.5 mgl -1 2,4-D and 0.5 mgl -1 Kn. In this treatment, the highest number of shoot induction per callus was 6.0 ±1.0. The callus derived shoots rooted on MS medium containing 1.0 mgl -1 IBA within ten days of culture. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival.

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In vitro Regeneration of the Medicinal Herb of Nilgiri shola, Acmella calva L. from Leaf Derived Callus

Cited by 6 publications

References 6 publications

In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker

In vitro clonal propagation and genetic fidelity of the regenerants of Spilanthes calva DC. using RAPD and ISSR marker

Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

Studies on <i>In vitro</i> Callus Induction and Regeneration from Nodal Explants of <i>Cassia alata</i> L.

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