In vitro regeneration of the endangered cactus Turbincarpus mombergeri (Riha), a hybrid of T. laui × T. pseudopectinatus

Abstract

Turbinicarpus mombergeri is a cacti species formed by a hybridization process between Turbinicarpus laui and Turbinicarpus pseudopectinatus. Under natural conditions, it is very di cult for two species be genetically compatible for hybridization, and to produce owers at the same time. Thus, T. mombergeri is a very interesting and a rare species. Unfortunately, the current populations are decreasing and now it is considered critically endangered. The aim of this research was to develop a successful protocol for propagating T. mombergeri using the in vitro culture techniques. Seed disinfection was performed with Plant Preservative Mixture, and 80% of germination occurred at day 45 in Murashige-Skoog medium. The shoots were cut longitudinally, and the segments were transferred to media containing 2.22 or 4.44 µM benzyladenine to induce shooting. The generated shoots were highly hydrated, and presented abundant callus. The hyperhydricity was controlled by reducing salt medium concentration, by increasing calcium levels and by using polyethylenglycol. The reduction of callus was attained by adding tri-iodo benzoic acid. Vigorous and thick shoots were generated in medium containing urea, and rooting improved in the presence of 0.5 µM indoleacetic acid. Plantlets with normal morphology were obtained, and the survival rate of the plants in soil was 80%. The methodology developed represents an alternative for propagation of T. mombergeri under controlled conditions for commercial or conservation purposes.

In Vitro Propagation and Genetic Uniformity Assessment of Manglietiastrum sinicum: A Critically Endangered Magnoliaceae Species

“…However, for some woody species, the effect of MS medium is negative because of its high levels of ammonium ions [33]. Many studies have indicated that the incidence of hyperhydricity can be minimized by reducing the salt concentration of the medium, especially for endangered plants [34]. Our results showed the growth and proliferation of buds to be much higher on MSM, in which the concentrations of ammonium nitrogen (1280 mg/L) and nitrate nitrogen (2800 mg/L) were moderate.…”

Effects of plant preservative mixture™ on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its application in orchid conservation

“…To date, in vitro shoot propagation or asymbiotic seed germination is considered a reliable method for the conservation of orchids (Hoang et al 2016;Pujasatria et al 2020;Santos-Díaz et al 2022). Especially the number of explants that are available for in vitro conservation projects can be seasonal or very limited in quantity (Sarasan 2010).…”