In vitro regeneration of wild chervil (Anthriscus sylvestris L.)

Hendrawati, Oktavia; Hille, Jacques; Woerdenbag, Herman J.; Quax, Wim J.; Kayser, Oliver

doi:10.1007/s11627-011-9410-3

Cited by 10 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lowest tested concentration used (0.1 mg l -1 zeatin) provided the highest number of shoots per plant from all 26 tested media. Superior effect of zeatin over kinetin and BA had been reported also for micropropagation of other species (Peixe at al., 2007;Hendrawati et al, 2012). Although zeatin is very expensive chemical compound, it is the only growth regulator capable of inducing satisfactory growth and multiplication, and thus it is not possible to replace it by another cytokinin.…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Rejthar¹,

Viehmannová

Čepková³

et al. 2014

Emir. J. Food Agric

View full text Add to dashboard Cite

This study was aimed to optimize in vitro propagation of Drosera intermedia for commercial and conservation purposes. The effect of concentration of MS nutrients (⅛ MS, ¼ MS, ½ MS and, MS), various pH (3.7-7.7), sucrose concentration (10-40 g l -1 ) and cytokinins (0.1-3 mg l -1 ), namely BA (N6-benzyladenine), kinetin and zeatin were evaluated. After 60 days of shoot cultivation, growth and developmental characteristics (plant height, number of shoots per explant, diameter of rosette, number of roots per explant, length of roots) were recorded. No significant differences were found for various levels of pH and sucrose. On the contrary, plant height was negatively influenced by an increase of nutrients in the medium. The plants on 1/8 MS medium were significantly taller, and displayed higher proliferation capacity compared with those cultivated on full-strength MS medium. Shoot multiplication and growth was suppressed by supplementation of BA and kinetin, regardless of concentration used. Zeatin at the lowest concentration (0.1 mg l -1 ) provided the best results for shoot proliferation of all 26 treatments and can be recommended for micropropagation of D. intermedia.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Rejthar¹,

Viehmannová

Čepková³

et al. 2014

Emir. J. Food Agric

View full text Add to dashboard Cite

show abstract

“…Plant tissue culture, or the aseptic culture of cells, tissues, organs, and their components under defined physical and chemical conditions in vitro , is an important tool in both basic and applied studies as well as in commercial application [117]. Calli of A. sylvestris did not produce deoxypodophyllotoxin and whole plants were required in order to achieve the differentiated forms to produce deoxypodophyllotoxin for metabolic engineering purposes [118]. In the cell suspension cultures only trace amounts of deoxypodophyllotoxin were detected.…”

Section: Biotechnology Applicationsmentioning

confidence: 99%

Ethnomedicinal, Phytochemical and Pharmacological Profile of Anthriscus sylvestris as an Alternative Source for Anticancer Lignans

et al. 2015

View full text Add to dashboard Cite

Anthriscus sylvestris (L.) Hoffm. is a wild herbaceous plant common in most temperate regions. It has been used traditionally to treat headaches, as a tonic, as antitussive, antipyretic, analgesic and diuretic. The plant contains deoxypodophyllotoxin, which is proven to have antitumor and anti-proliferative effects, anti-platelet aggregation, antiviral, anti-inflammatory and insecticidal activity. Deoxypodophyllotoxin is considered to be the plant's most important constituent, because of its pharmacological properties and because it can be converted into epipodophyllotoxin, the main raw material for the semisynthesis of the cytostatic agents etoposide and teniposide. This work summarizes for the first time the results related to the botanical description, distribution and habitat, phytochemical and pharmacological properties and emphasizes the aspects for future biotechnological research to establish its utility in the therapeutic arsenal.

show abstract

“…The substrates usually used for acclimatization with other members of that family (Sharma and Wakhlu 2001, Nath and Buragohain 2003, Tavares et al 2010a, Coste et al 2012, Hendrawati et al 2012, Jaheduzzaman et al 2012 were far different from the substrate which promoted acclimatization of our plant species. H. pastinacifolia did not acclimatize at all if the substrates were rich in organic matter (data not shown).…”

Section: Acclimatization and Transfer To In Vivo Conditionsmentioning

confidence: 95%

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

Ambrožič-Dolinšek¹,

Ciringer²,

Kaligarič³

2016

Acta Botanica Croatica

View full text Add to dashboard Cite

-The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds and juvenile plants obtained from natural sites on a solid Murashige and Skoog (MS) medium, with and without growth regulators. We tested various combinations and concentrations of growth regulators, and the best proliferation of axillary shoots, on average 14, was obtained on MS medium with 5 μM BAP and 3 μM IBA and 3% sucrose. Rooting was achieved after transferral of the shoots to an MS medium with 2 μM IBA and 3% sucrose. The rooted plants were acclimatized on a mixture of limestone sand, potting soil and vermiculite in a ratio of 10:2:2, with pH in the range of 7.5-8.0. In vitro propagation methods provide an important opportunity for the propagation and preservation of H. pastinacifolia by rapidly increasing the number of plants, without disturbing the wild population.

show abstract

In vitro regeneration of wild chervil (Anthriscus sylvestris L.)

Cited by 10 publications

References 26 publications

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Ethnomedicinal, Phytochemical and Pharmacological Profile of Anthriscus sylvestris as an Alternative Source for Anticancer Lignans

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

Contact Info

Product

Resources

About