In vitro regeneration protocol of Rauvolfia serpentina L.

Khan, Salim; Banu, TA; Akter, Shahina; Goswami, Barna; Islam, Md. Ekramul; Hani, Umme; Habib, Ahsan

doi:10.3329/bjsir.v53i2.36674

Cited by 15 publications

(11 citation statements)

References 11 publications

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“…1). Khan et al (2018) also found shoot regeneration on MS with BAP and IAA using leaf explants in Rawolfia. George and Rao (1980) also observed maximum regeneration from cotyledon explants in B. juncea with combinations of BAP.…”

Section: Resultsmentioning

confidence: 85%

In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

Goswami

Hoque

Khan

et al. 2020

Bangladesh J. Sci. Ind. Res.

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A reproducible in vitro regeneration system was developed for three varieties of Brassica campestris L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly in vitro regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced in vitro flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the in vitro regenerated plantlets were successfully transplanted into soil. Bangladesh J. Sci. Ind. Res.55(3), 181-188, 2020

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Section: Resultsmentioning

confidence: 85%

In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

Goswami

Hoque

Khan

et al. 2020

Bangladesh J. Sci. Ind. Res.

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“…Banu et al (2017) also observed better response in Gynura procumbens on MS with BAP, Kn and IAA supplemented medium. Khan et al (2018) also reported that combination of 2.0 mg/l BAP, 0.5 mg/l IAA and 0.02 mg/l NAA was best for the multiple shoot formation from nodal segments in Rauvolfia serpentina. Bhat et al (1995) showed that BA alone was more effective than Kn for different Piper spp.…”

Section: Resultsmentioning

confidence: 91%

In vitro mass propagation of Piper betle L

et al. 2019

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An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.

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“…Rani et al [1] reported axillary shoots of R. serpentina exhibited regeneration of maximum number of shoots on MS medium containing IAA and BAP. In another micropropagation study conducted by Khan et al [19] on R. serpentina, MS + BAP + NAA was found to be effective enough to induce direct regeneration of shoots from nodal segments and development of callus, followed by shoot regeneration from in vitro cultured leaf segments. In their study, regeneration from nodal and leaf segments was also achieved on MS + BAP + Kn and MS + BAP + IAA.…”

Section: Micropropagation and Phytochemical Analysis Of Rauvolfia Sermentioning

confidence: 93%

Micropropagation and Screening of Phytocompounds Present among in vitro Raised and Wild Plants of Rauvolfia serpentine

Varnika

Sharma

Singh

et al. 2020

Walailak J Sci & Tech

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Rauvolfia serpentina, commonly known as Sarpgandha, is a plant well-known for being utilized for several medicinal purposes. Unrestricted collection from natural stands and overexploitation for medicinal and traditional purposes have rendered it endangered, hence there is necessary requirement for the development of cultivation protocols for mass propagation and sustained utilization of the plant. In the present study, in vitro culture of the apical parts of R. serpentina on MS (Murashige and Skoog) medium enriched with NAA resulted in development of callus, whereas multiple shoot regeneration along with callus development was achieved on medium combination MS + NAA + Kn and MS + NAA + BAP. MS + 4µM NAA + 4µM BAP was found to be most optimum media composition for regeneration of shoots and callus. Among different media combinations utilized for in vitro rooting, a maximum of 82.6 % explants developed in vitro roots on ½ MS + 12µM NAA. Gas chromatography-mass spectrometry (GC-MS) analysis of methanolic extract prepared from leaves of wild and micropropagated plants of R. serpentina revealed the presence of 38 and 48 phytocompounds, respectively. 9, 12-Octadecadienoic acid, Methyl linoleate, Methyl stearate, Hexadecanoic acid, methyl ester, Linoleic acid, Ergost-4, 7, and 22-trien-3.alpha.-ol were some of the major compounds found to be present in the leaves of wild plants, and Linoleic acid, methyl ester, Cis-Linoleic Acid Methyl Ester, Methyl elaidate, hexadecanoic acid, and methyl ester were major compounds found to be present in in vitro raised plants. Many of the compounds detected have been known to possess 1 or more biological or pharmacological activities.

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In vitro regeneration protocol of Rauvolfia serpentina L.

Cited by 15 publications

References 11 publications

In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

In vitro regeneration of three varieties of <i>Brassica campestris L.</i> grown in Bangladesh

In vitro mass propagation of Piper betle L

Micropropagation and Screening of Phytocompounds Present among in vitro Raised and Wild Plants of Rauvolfia serpentine

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