In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma

Abstract

The implantation of biomedical devices, including collagen-based implants, evokes an inflammatory response. Despite inflammation playing an important role in the early stages of wound healing, excessive and non-resolving inflammation may lead to the poor performance of biomaterial implants in some patients. Therefore, steps should be taken to control the level and duration of an inflammatory response. In this study, oxygen and nitrogen gas plasmas were employed to modify the surface of collagen film, with a view to modifying the surface properties of a substrate in order to induce changes to the inflammatory response, whilst maintaining the mechanical integrity of the underlying collagen film. The effects of cold plasma treatment and resultant changes to surface properties on the non-specific inflammatory response of the immune system was investigated in vitro in direct contact cell culture by the measurement of protein expression and cytokine production after one and four days of human peripheral blood mononuclear cell (PBMC) culture. The results indicated that compared to oxygen plasma, nitrogen plasma treatment produced an anti-inflammatory effect on the collagen film by reducing the initial activation of monocytes and macrophages, which led to a lower production of pro-inflammatory cytokines IL-1β and TNFα, and higher production of anti-inflammatory cytokine IL-10. This was attributed to the combination of the amino chemical group and the significant reduction in roughness associated with the introduction of the nitrogen plasma treatment, which had an effect on the levels of activation of the adherent cell population.

Methacrylated Gellan Gum/Poly-l-lysine Polyelectrolyte Complex Beads for Cell-Based Therapies

“…While IL-8 and IL-1β are related to immune rejection, IL-10 inhibits the production of different pro-inflammatory cytokines, including IL-8 and IL-1β, and is associated with immune acceptance . In this regard, the results obtained for donor 2 point to a controlled immune response, where the secretion of pro- and anti-inflammatory cytokines is balanced . However, it must be stressed out that none of the values were above the ones measured when in presence of lipopolysaccharide, the positive control.…”

“…60 In this regard, the results obtained for donor 2 point to a controlled immune response, where the secretion of pro-and anti-inflammatory cytokines is balanced. 63 However, it must be stressed out that none of the values were above the ones measured when in presence of lipopolysaccharide, the positive control. This result suggests that GG-MA/PLL PEC beads do not exerted per se a serious inflammatory reaction that could compromise the functionality of this system as an immunoprotective matrix for cells.…”

In-Vitro Biofilm Removal Efficacy Using Water Jet in Combination with Cold Plasma Technology on Dental Titanium Implants

“…In our former study [ 58 ], we suggested not to analyse the specimen directly after treatment because this approach can overestimate antimicrobial performance, which can be circumvented either by longer incubation [ 58 ] or by the cultivation of osteoblasts on treated discs [ 70 ]. This approach works with discs but not with implants because it is difficult to protect cleaned from contaminated areas in the latter.…”

In Vitro Biocompatibility Evaluation of a New Co-Cr-B Alloy with Potential Biomedical Application

“…hPBMCs have been widely used as an in vitro model to investigate biocompatibility and inflammation; thus, they were used in this work [22]. The hPBMCs were extracted using a density gradient [23].…”