In vitro response of mesenchymal stem cells to biomimetic hydroxyapatite substrates: A new strategy to assess the effect of ion exchange

Sadowska, Joanna M.; Guillem-Marti, Jordi; Español, Montserrat; Stähli, Christoph; Döbelin, Nicola; Ginebra, Maria‐Pau

doi:10.1016/j.actbio.2018.06.025

Cited by 41 publications

(45 citation statements)

References 54 publications

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“…Sadowska and colleagues addressed the topic of cell–biomaterial interaction from an interesting point of view, which is the effect of ion exchange [ 75 ]. They underlined that a different composition and topography of the material could result in a different extent of ion exchange, which would affect cell behavior (particularly in static conditions), as cells uptake ions from the surrounding fluids.…”

Section: Msc-scaffold Interaction: Mechanosensing and Mechanotransmentioning

confidence: 99%

“…They underlined that a different composition and topography of the material could result in a different extent of ion exchange, which would affect cell behavior (particularly in static conditions), as cells uptake ions from the surrounding fluids. Specifically, these authors found that, in the presence of highly reactive substrates, a low ratio between the cell culture medium and the biomaterial caused lower cell adhesion, due to a reduced number of focal adhesions, cell shrinkage, reduced proliferation, and increased apoptosis [ 75 ]. Actually, these adverse effects could be prevented by exploiting bioreactor systems in which tuning of fluid flow could optimize soluble factor exchange [ 37 , 76 , 77 ].…”

Section: Msc-scaffold Interaction: Mechanosensing and Mechanotransmentioning

confidence: 99%

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3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

Sobacchi

Erreni

Strina

et al. 2018

IJMS

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Mesenchymal stem cells (MSCs) are recognized as an attractive tool owing to their self-renewal and differentiation capacity, and their ability to secrete bioactive molecules and to regulate the behavior of neighboring cells within different tissues. Accumulating evidence demonstrates that cells prefer three-dimensional (3D) to 2D culture conditions, at least because the former are closer to their natural environment. Thus, for in vitro studies and in vivo utilization, great effort is being dedicated to the optimization of MSC 3D culture systems in view of achieving the intended performance. This implies understanding cell–biomaterial interactions and manipulating the physicochemical characteristics of biomimetic scaffolds to elicit a specific cell behavior. In the bone field, biomimetic scaffolds can be used as 3D structures, where MSCs can be seeded, expanded, and then implanted in vivo for bone repair or bioactive molecules release. Actually, the union of MSCs and biomaterial has been greatly improving the field of tissue regeneration. Here, we will provide some examples of recent advances in basic as well as translational research about MSC-seeded scaffold systems. Overall, the proliferation of tools for a range of applications witnesses a fruitful collaboration among different branches of the scientific community.

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Section: Msc-scaffold Interaction: Mechanosensing and Mechanotransmentioning

confidence: 99%

Section: Msc-scaffold Interaction: Mechanosensing and Mechanotransmentioning

confidence: 99%

3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

Sobacchi

Erreni

Strina

et al. 2018

IJMS

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“…These findings of lowered early osteogenic gene expression in the presence of aCaP nanoparticles under static conditions stand in contrast to our expectations. It has to be emphasized, however, that under static conditions the volume of the media:volume of the scaffold‐ratio ( V M : V S ) was much smaller than in the bioreactor . As a consequence, release/precipitation of free Ca ions from/to the scaffold material might have been attenuated in the bioreactor where the ratio V M : V S is approximately 1600, while it is only around 50 under static conditions .…”

Section: Discussionmentioning

confidence: 99%

Cartilage/bone interface fabricated under perfusion: Spatially organized commitment of adipose‐derived stem cells without medium supplementation

et al. 2018

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Tissue engineering of an osteochondral interface demands for a gradual transition of chondrocyte-to osteoblastprevailing tissue. If stem cells are used as a single cell source, an appropriate cue to trigger the desired differentiation is the use of composite materials with different amounts of calcium phosphate. Electrospun meshes of poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/aCaP) in weight ratios of 100:0; 90:10, 80:20, and 70:30 were seeded with human adipose-derived stem cells (ASCs) and cultured in DMEM without chemical supplementation. After 2 weeks of static cultivation, they were either further cultivated statically for another 2 weeks (group 1), or placed in a Bose ® bioreactor with a flow rate per area of 0.16 mL cm −2 min −1 (group 2). Markers for stem cell criteria, chondrogenesis, osteogenesis, adipogenesis and angiogenesis were analyzed by quantitative real-time PCR. Cell distribution, Sox9 protein expression and proteoglycans were assessed by histology. In group 2 (perfusion culture), chondrogenic Sox9 was upregulated toward the cartilage-mimicking side compared to pure PLGA. On the bonemimicking side, Sox9 experienced a downregulation, which was confirmed on the protein level. Vice versa, expression of osteocalcin was upregulated on the bone-mimicking side, while it was unchanged on the cartilage-mimicking side. In group 1 (static culture), CD31 was upregulated in the presence of aCaP compared to pure PLGA, whereas Sox9 and osteocalcin expression were not affected. aCaP nanoparticles incorporated in electrospun PLGA drive the differentiation behavior of human ASCs in a dose-dependent manner. Discrete gradients of aCaP may act as promising osteochondral interfaces.

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“…The blurry staining observed in Fine-and Coarse-CDHA indicates a poorly organized cytoskeleton usually associated to immature focal adhesions and reduced cell proliferation. 56 However, the RT-qPCR analysis showed, as a general trend, higher expression of osteogenic genes for the cells cultured on Fine-and Coarse-CDHA than on CO -CDHA samples, with no significant differences between the two undoped CDHA substrates (Supporting Information, Figure S2). This enhanced differentiation may also contribute to the reduction in cell proliferation.…”

Section: Effect Of Nanostructure and Carbonate Doping On Osteoinductionmentioning

confidence: 94%

Impact of Biomimicry in the Design of Osteoinductive Bone Substitutes: Nanoscale Matters

Barba

Díez-Escudero²,

Español³

et al. 2019

ACS Appl. Mater. Interfaces

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Bone apatite consists in carbonated calcium deficient hydroxyapatite nanocrystals (CDHA). Biomimetic routes allow fabricating synthetic bone grafts that mimic biological apatite. In this work, we explored the role of two distinctive features of biomimetic apatites, namely nanocrystal morphology (plate vs. needle-like crystals) and carbonate content on the bone regeneration potential of CDHA scaffolds in an in vivo canine model. Both ectopic bone formation and scaffold degradation were drastically affected by the nanocrystal morphology after intramuscular implantation. Fine-CDHA foams with needle-like nanocrystals, comparable in size to bone mineral, showed a markedly higher osteoinductive potential and a superior degradation than chemically identical Coarse-CDHA foams with larger plate-shaped crystals. These findings correlated well with the superior bone healing capacity showed by the Fine-CDHA scaffolds when implanted intraosseously. Moreover, carbonate doping of CDHA, which resulted in small plate-shaped nanocrystals, accelerated both the intrinsic osteoinduction and the bone healing capacity, and significantly increased the cell-mediated resorption. These results suggest that tuning the chemical composition and the nanostructural features may allow the material to enter the physiological bone remodelling cycle, promoting a tight synchronization between scaffold degradation and bone formation.

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In vitro response of mesenchymal stem cells to biomimetic hydroxyapatite substrates: A new strategy to assess the effect of ion exchange

Cited by 41 publications

References 54 publications

3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells

Cartilage/bone interface fabricated under perfusion: Spatially organized commitment of adipose‐derived stem cells without medium supplementation

Impact of Biomimicry in the Design of Osteoinductive Bone Substitutes: Nanoscale Matters

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