In vitro Response of Some Banana Cultivars to Salicylic AcidTreatment Under Salinity Stress

Abstract

The effect of Salicylic acid (SA) on three Cavendish banana cultivars (i.e. Zeef, imported and local Grand Naine) subjected to in vitro salt stress was evaluated. Five concentrations of NaCl (0,30,60,120 and 200 mM) were tested, among which the optimum concentration for screening was determined at 120 mM. Different concentrations of SA (0, 0.5 and 1 mM) were assessed for several vegetative and physiological traits of the three cultivars cultured upon medium containing 120 mMNaCl. Analysis of variance showed highly significant differences among cultivars, SA concentrations and salinity levels, as well as their interactions in most of the studied traits. NaCl treatment caused severe loss in vegetative and physiological traits in all tested cultivars and activated several protein bands compared with non-saline conditions. Moreover, addition of SA exhibited a beneficial effect in reducingdamage caused by salinity in several traits i.e. survival percentage, plantlet length, fresh weight, total chlorophyll, carotenoids and proline content and Potassiumuptake. In addition, SA at 0.5 mM was more effective in enhancement salinity tolerance, while high concentration inhibited most of protein bands enhanced under salinity. The results showed that among the evaluated cultivars, both Grand Naine cultivars were more tolerantthanZeef cultivar. The information resulted herein is valuable and could be help inimprovement of salt tolerance in banana.

Influence of Sodium Salicylate on Adventitious Organogenesis of a Commercial Cucumber Cultivar

“…In addition, other plant processes and attributes reported, in particular with NaSA application, may also influence regeneration efficiency, such as changes in photosynthetic pigment content (Elazab and Youssef 2017;Klimek-Szczykutowicz et al 2022) and net photosynthetic and respiration rates (Al-Hakimi 2001), beneficial ion uptake and transport (Al-Hakimi and Hamada 2001;Elazab andYoussef 2017), enhanced growth (Al-Hakimi 2001;Elazab and Youssef 2017) and biomass parameters (Bhambhani et al 2012;Klimek-Szczykutowicz et al 2022), improved plant water relations (Al-Hakimi 2001), changes in secondary metabolite content (Silja and Satheeshkumar 2015;Klimek-Szczykutowicz et al 2022), and so forth.…”

“…In some reports, the efficacy of applied SA/salicylates increased under stress conditions. For example, in in vitro propagation of three Cavendish banana (Musa acuminata Colla) cultivars, NaSA treatments under non-saline conditions were generally less effective than under salinity (120 mM NaCl) on the vegetative and physiological traits evaluated (Elazab and Youssef 2017).…”

“…Exogenous application of SA may affect a variety of plant processes and attributes, such as photosynthesis and photosynthetic pigments (Khan et al 2003;Maurya et al 2019), respiratory pathways (Khan et al 2003), ethylene biosynthesis (Khan et al 2013), seed germination (Lee et al 2010), flower induction (Cleland and Tanaka 1979), thermogenesis (Rhoads and McIntosh 1992), ion uptake and transport (Harper and Balke 1981;Gondor et al 2016), nitrate reductase activity (Fariduddin et al 2003), protein content (Latif et al 2016), growth parameters (Damalas 2019), biomass production (Maurya et al 2019), plant water relations (Hayat et al 2010), stomatal closure (Khan et al 2003), accumulation of osmolytes (e.g., proline, glycine betaine, polyamines, and soluble sugars) (Khan et al 2014;Madany et al 2020;Shemi et al 2021), secondary metabolite content (e.g., alkaloids, flavonoids, and phenolics) (for review see Nandy et al 2021), allelopathic properties (Shettel and Balke 1983), antioxidant defense system (Maurya et al 2019), and senescence (Rivas-San Vicente and Plasencia 2011). It can also alleviate environmental stresses, such as heat (Khan et al 2013), cold (Saleem et al 2020), drought, (Sohag et al 2020), UV-B/C radiation (Li et al 2014;Abrun et al 2016), salinity (Elazab and Youssef 2017), and heavy metals toxicity (Sharma et al 2020), and protect plants from a range of pathogens (Bakker et al 2014). The effect of exogenous SA is influenced by factors such as the plant species, its stage of development, tissue and organ type, mode of application, dose and duration of exposure, environmental and culture conditions, and its endogenous level in the plant (Horváth et al 2007;…”