In vitro response to oxygen of human umbilical arteries and of animal ductus arteriosus

Bor, Imrich; Guntheroth, Warren G.

doi:10.1139/y70-077

Cited by 20 publications

(7 citation statements)

References 11 publications

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“…Investigation into the reactivity of the human umbilical artery (HUA) has shown that increasing the level of oxygen above 16 mmHg will induce a transient contraction which can be blocked by various cyclo-oxygenase inhibitors (MacLennan et al, 1988). This confirmed earlier work (Lewis, 1968;Bor & Guntheroth, 1970;Oberhansli-Weiss et al, 1972; as to the contractile effect of oxygen but also suggested that oxygen's effect was mediated via prostaglandins.…”

Section: Introductionsupporting

confidence: 72%

The role of endogenous thromboxane in contractions to U46619, oxygen, 5‐HT and 5‐CT in the human isolated umbilical artery

Templeton

McGrath

Whittle

1991

British J Pharmacology

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1 The effects of selective thromboxane antagonists and a thromboxane synthase inhibitor on the contraction to 9,11-dideoxy-1la,9a-epoxymethano-prostaglandin F2. (U46619) and oxygen in the human umbilical artery (HUA) were examined. The effect of the antagonists on contractions to both 5-hydroxytryptamine (5-HT) and 5-carboxamidotryptamine (5-CT) were also examined. 2 U46619 (0.3 nM-10uM) contracted the HUA. This contraction was antagonized by two selective thromboxane receptor antagonists EP092 (10 nM-l uM) and GR32191B (10 nM-I uM). The contraction was not affected by the selective thromboxane synthase inhibitor, dazoxiben (10 nM-1 pM).3 When the oxygen tension was increased from 16 mmHg to 120 mmHg, the HUA transiently contracted. Both thromboxane antagonists inhibited this contraction in a concentration-dependent manner with 1,UM almost completely abolishing the response (the oxygen-induced contraction of the control preparation normally increases with a second exposure to 120 mmHg oxygen). 4 In low (16 mmHg) oxygen, responses to both 5-HT and 5-CT were unaffected by both thromboxane receptor antagonists at concentrations qp to 1 UM. In high oxygen (120 mmHg) responses to both 5-HT and 5-CT were biphasic in nature, with an additional initial high sensitivity phase, which was abolished by a cyclo-oxygenase inhibitor. In high oxygen, EP092 and GR32191B blocked this initial phase in a concentration-dependent manner, returning sensitivity to 5-HT and 5-CT to that seen in low oxygen. 5 The thromboxane synthase inhibitor, dazoxiben, at concentrations greater than 10 nm inhibited the contraction to 120 mmHg oxygen and at 1 jUM, dazoxiben almost abolished the response. In low oxygen, the response to 5-HT was unaffected by dazoxiben at concentrations up to 10piM. In high oxygen, the initial phase of the contraction to 5-HT was inhibited by concentrations greater than 10 nm, with no effect on the maximum response. 6 The results show that thromboxane receptor antagonism or blockade of thromboxane synthesis selectively attenuates oxygen-induced contractions and those responses to 5-HT and 5-CT which are dependent on high oxygen for their expression. This suggests that the contractions caused by high oxygen tension, and the enhancement of the contractile effects of low concentrations of 5-HT and 5-CT in the presence of high oxygen tension are mediated by endogenously released thromboxane A2.

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Section: Introductionsupporting

confidence: 72%

The role of endogenous thromboxane in contractions to U46619, oxygen, 5‐HT and 5‐CT in the human isolated umbilical artery

Templeton

McGrath

Whittle

1991

British J Pharmacology

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“…With in vitro studies, the contraction of smooth muscle of the arterial duct is prompt, following within five minutes an increase in the perfusing PO 2 [1]. In normal infants, the first breath produces a pulse of oxygen to the pulmonary parenchyma, which causes the pulmonary vascular resistance to fall, permitting a left-toright shunt through the duct, which stimulates contraction of the media of the duct.…”

Section: Discussionmentioning

confidence: 99%

The Role of Ductal Constriction in Transient Stenosis of the Left Pulmonary Artery

Guntheroth

1998

Pediatr Cardiol

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To confirm the hypothesis that narrowing of the left pulmonary artery is produced by ductal closure, Doppler velocities in the pulmonary arteries from 20 premature infants with a patent duct were compared to velocities from 20 with a closed duct. In infants with a patent duct, the mean Doppler velocity in the main, right, and left pulmonary arteries were, respectively, 86 cm/sec +/- 5 standard error (SE), 120 +/- 7, and 125 +/- 8. With closed duct, the comparable velocities were 102 cm/sec +/- 7, 129 +/- 8, and 190 +/- 11. The only significant difference was the higher velocity in the left pulmonary artery associated with a closed duct (p = 0.00003). This physiologic origin of left-branch stenosis associated with ductal constriction should be considered when a murmur is detected after administration of indomethacin (in addition to possible persistent ductal flow), and as an explanation for some murmurs in the normal newborn. No anatomic or physiologic support was found for the theories that murmurs in the neonate were due to ductal flow, or to acute angulation of the branch pulmonary arteries.

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“…The artery in vitro constricts when exposed to oxygen (3)(4)(5)(6). In addition, by virtue of their ability to produce constriction in vitro as well as their actual or presumed concentrations in cord blood at birth, bradykinin, serotonin, histamine, acetylcholine, prostaglandins A2 and F2 have been suggested as probable chemical mediators of umbilical artery closure (4)(5)(6)(7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Guanosine 3',5'-monophosphate and adenosine 3',5'-monophosphate content of human umbilical artery.

Clyman¹,

Sandler²,

Manganiello³

et al. 1975

J. Clin. Invest.

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In vitro response to oxygen of human umbilical arteries and of animal ductus arteriosus

Cited by 20 publications

References 11 publications

The role of endogenous thromboxane in contractions to U46619, oxygen, 5‐HT and 5‐CT in the human isolated umbilical artery

The role of endogenous thromboxane in contractions to U46619, oxygen, 5‐HT and 5‐CT in the human isolated umbilical artery

The Role of Ductal Constriction in Transient Stenosis of the Left Pulmonary Artery

Guanosine 3',5'-monophosphate and adenosine 3',5'-monophosphate content of human umbilical artery.

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