In vitro responses of raspberry plantlets to the culture media enriched with some plant sprout powders

Abstract

The growth and morphogenesis responses of cultured cell and plant tissues can be improved by addition of small amounts of some organic materials like plant sprout powders (PSP). A preliminary experiment was undertaken to examine the response of raspberry plantlets to media enriched with plant sprout powders derived from wheat, vetch and alfalfa plant species. The Murashige and Skoog (MS) medium supplemented with IBA (2.0 mg/l) and activated charcoal (200 mg/l) was exploited as optimized shoot proliferation for raspberry. Seeds of wheat, vetch or alfalfa were subjected to germination and when the rootlets attained 1 cm long, the sprouts were dried and ground to provide a homogenous powder. The shoot proliferation medium already optimized for raspberry was supplemented with 1.0 g/l of each wheat, vetch, or alfalfa sprout powders. The double-node cuttings of in vitro grown raspberries were inoculated on these media. Therefore, the growth responses of raspberries were measured in media with or without PSP. The results revealed the positive response of raspberry plantlets to the medium containing vetch sprout powder and most of the growth parameters including number of shoots, leaves, roots, and plantlet length were improved. Besides obvious apparent quality of in vitro cultures and better growth performance, the plantlets grown on media enriched with vetch sprout powder showed highest leaf number (6.41) and longer internodal length (1.89 mm). Therefore, the obtained data encourage the utilization of PSP in plant tissue culture media.

In vitro propagation of Zamoculcas zamipholia LODD., through indirect regeneration

“…However, the slow growth habit of ZZ plant (Henny and Chen, 2013) is a drawback for its mass propagation. Tissue culture techniques provide an opportunity for the large-scale production of an elite material and plants of commercial interest (Alizadeh et al, 2023).The micropropagation protocols for some Araceae genera such as Alocasia, Anthurium, Aglaonema, Dieffenbachia, Philodendron, Spathiphyllum and Syngonium has been already reported Stanly et al, 2012;Chen et al, 2018;Chen et al, 2012). Also, there are some contributions for in vitro propagation of green leaved Zamiifolia.…”