In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity

Kusampudi, Shilpa; Selvakkumar, Chinnasamy; Senthil, Arun Kumar; Lakshmi, Baddireddi Subhadra

doi:10.1007/s11240-009-9661-4

Cited by 17 publications

(13 citation statements)

References 18 publications

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“…In the present study, however, no such inhibitory effect of cytokinins was noticed and production of healthy fasciculated roots took place under the combined influence of BA and Kn. Reports published earlier have demonstrated that Kn promotes the development of roots (Hill and Lawrence 1985) and a low dose of Kn in combination with NAA has been found to be highly conducive for induction of roots from young leaf explants of Ocimum sanctum (Shilpa et al 2010). Our reports are also in agreement with the previous reports describing the promotive effects of BA and Kn on root growth and proliferation.…”

Section: Resultssupporting

confidence: 93%

In vitro Root Culture : An Alternative Source of Bioactives in the Rare Aphrodisiac Herb Chlorophytum borivilianum Sant et Fern

Basu¹,

Jha²

2014

Plant Tissue Cult. & Biotech.

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Chlorophytum borivilianum is a rare, near-endemic high value root crop. The fasciculated roots of the plant have the potential to synthesize valuable bioactives having aphrodisiac, anti stress and immuno modulatory properties. The present investigation describes an alternative protocol for the development of a stable, fast growing, non-transformed root culture system in C. borivilianum and the simultaneous quantitative analysis of total steroidal saponins, amino acids and sugars. Liquid MS fortified with various concentrations and combinations of BA (0.44 -8.8 μM), Kn (0.23 μM) and NAA (0.27 -5.4 μM) was effectively used for the development of root culture system. High performance thin layer chromatography (HPTLC) was used for quantification and comparative analysis of bioactives from in vitro and in vivo grown tuberous roots.

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Section: Resultssupporting

confidence: 93%

In vitro Root Culture : An Alternative Source of Bioactives in the Rare Aphrodisiac Herb Chlorophytum borivilianum Sant et Fern

Basu¹,

Jha²

2014

Plant Tissue Cult. & Biotech.

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“…The production of annatto pigment from normal root cultures that has been reported in this study is unique compared with the other root cultures reported to produce secondary metabolites, such as Hemidesmus indicus (Sreekumar et al 1998), Decalepis hamiltonii (Giridhar et al 2005), Angelica gigas (Rhee et al 2010), and antioxidant activity as in the case of Ocimum sanctum (Shilpa et al 2009). Similarly, liposoluble pigments from edible roots of Beta vulgaris (Kolesnik et al 1989) and localization of carotenoids in roots of graminaceous plants (Klingner et al 1995) were previously known.…”

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confidence: 53%

Annatto pigment production in root cultures of Achiote (Bixa orellana L.)

Gondi

Venugopalan

Rangan

et al. 2011

Plant Cell Tiss Organ Cult

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Bixa orellana L. is a tree native to South America known for its reddish orange pigment 'annatto' produced only on the aril portion of its seeds. It is the most preferred natural food colorant next to saffron, having wide applications in the dairy industry and also as a cosmeceutical. Normal root cultures of B. orellana were established under in vitro conditions on Murashige and Skoog (MS) medium containing a-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) at 0.05-0.2 mg l -1 . The annatto pigment from in vitroraised normal roots was extracted with chloroform, and later the ethanol-dissolved extract was analyzed both qualitatively by thin-layer chromatography (TLC) and spectrophotometrically quantified followed by High Performance Liquid Chromatography (HPLC) confirmation. The maximum amount of annatto pigment (346 ± 3.8 mg/ 100 g dry wt.) and maximum root biomass (152 ± 2.5 mg dry wt.) were recorded after 45 and 60 days of growth, respectively, on MS medium containing 0.1 mg l -1 indole-3-butyric acid (IBA). Producing annatto pigment from normal root cultures under in vitro conditions is a novel approach when compared to the natural annatto pigment that is produced only on the aril portion of seeds. This allows the production of fresh pigment throughout the year.

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“…Regenerated plantlets are able to accumulate secondary metabolites similar to those found in the mother plant (Shilpa et al 2010). The antioxidant potential of the regenerated tissues of P. nigrum was determined using the DPPH8 free radical (Fig.…”

Section: Gibberellic Acid á Organogenesis á Piper Nigrummentioning

confidence: 99%

Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L.

Ahmad

Fazal

Abbasi

et al. 2010

Plant Cell Tiss Organ Cult

117

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The organogenic potential and antioxidant potential (1, 1-diphenyl-2-picrylhydrazyl-scavenging activity) of the medicinal plant Piper nigrum L. (black pepper) were investigated. Callus induction and shoot regeneration were induced from leaf explants of potted plants cultured on MS medium supplemented with different plant growth regulators. The best callogenic response was observed on explants cultured for 30 days on MS medium supplemented with either 0.5 or 1.5 mg l -1 6-benzyladenine (BA) ? 1.0 mg l -1 a-naphthaleneacetic acid. Subsequent transfer of the callogenic explants onto MS medium supplemented with 1.5 mg l -1 BA ? 1.0 mg l -1 gibberellic acid (GA 3 ) achieved 85% shoot organogenesis after 30 days of culture. The maximum number (7.2) of shoots/explant was recorded for explants cultured in MS medium supplemented with 1.0 mg l -1 BA. Following the transfer of shoots to an elongation medium, the longest shoots (5.4 cm) were observed on MS medium supplemented with 1.0 mg l -1 BA ? 1.0 mg l -1 GA 3 . The elongated shoots were rooted on MS medium supplemented with different concentrations of indole butyric acid. An assay of the antioxidant potential of the in vitro-grown tissues revealed that the antioxidant activity of the regenerated shoots was significantly higher than that of callus and the regenerated plantlets.

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In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity

Cited by 17 publications

References 18 publications

In vitro Root Culture : An Alternative Source of Bioactives in the Rare Aphrodisiac Herb Chlorophytum borivilianum Sant et Fern

In vitro Root Culture : An Alternative Source of Bioactives in the Rare Aphrodisiac Herb Chlorophytum borivilianum Sant et Fern

Annatto pigment production in root cultures of Achiote (Bixa orellana L.)

Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L.

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