In vitro root induction of faba bean (
 Vicia faba
 L.)

Ismail, Roba M.; Elazab, Heba Elazab Mohamed; Hussein, Gihan M. H.; Metry, E. A.

doi:10.4161/gmcr.2.3.18003

Cited by 9 publications

(4 citation statements)

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“…In a micro propagation system, the final culture stage prior to acclimatization is rooting (Ismail et al, 2011;Millán-Orozco et al, 2011). Survival of in vitro grown plantlets in the field depends on the development of a good rooting system to absorb water and nutrients from the soil (Benková and Bielach, 2010).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of in Vitro Rooting By Gelling Agents and Activated Charcoal in Rehmannia Glutinosa L.

Thwe¹,

Kim²,

Kim³

et al. 2015

OnLine Journal of Biological Sciences

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Plant tissue culture has proven to be commercially important for the replication of various plants species, including medicinal and ornamental plants. This technique has been used extensively in research for applications in agriculture and forestry. This study examines the effect of activated charcoal and the relative effectiveness of Gelrite compared to Phytagar. The number of roots per explant (8.2) and root growth (46.8 mm) was greater on Schenk and Hildebrandt medium containing 3 g/L Gelrite than that medium containing 7 g/L Phytagar which showed the number of roots per explant and root growth of 7.6 and 45.2 mm, respectively. Additionally, the highest regeneration rate (98%), the greatest number of roots per explant (8.8) and the longest root growth (71.5 mm) were achieved when 1 g/L of activated charcoal was added as a supplement to the medium. These findings provide useful information for future industrial-scale root production of Rehmannia glutinosa.

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Section: Introductionmentioning

confidence: 99%

Enhancement of in Vitro Rooting By Gelling Agents and Activated Charcoal in Rehmannia Glutinosa L.

Thwe¹,

Kim²,

Kim³

et al. 2015

OnLine Journal of Biological Sciences

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“…VILLORDON et al (2009) observed the adventitious root development, and suggested the initial stages of adventitious root development are critical in determining storage root set in sweetpotato. ISMAIL et al (2011) proved that the produced roots are initiated as the adventitious lateral root with tri-arch xylem strands as compared with the pentaarch of the primary roots of the intact faba bean seedling. MILLÁN-OROZCO et al (2011) observed the process of adventitious root formation with the eigthweek-old shoots of Cedrela odorata.…”

Section: Introductionmentioning

confidence: 98%

Anatomical and Physiological Effects of Phytohormones on Adventitious Roots Development in Larix kaempferi × L. olgensis

et al. 2013

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Adventitious root development is crucial for cutting propagation in hybrid larch. In the present study, semilignified cuttings of Larix kaempferi ϫ L. olgensis clones were used to study the morphological and anatomical changes during the different development stages and compare the changes in contents of endogenous phytohormone in two clones with evidently different rooting capacity (25-5 and 23-12), and the effects of exogenous Indole-3-Butyric Acid (IBA) on phytohormone content of a very low native rooting capacity clone (8-9) during the development of adventitious roots. The results showed that the development of adventitious root in semi-lignified cuttings involves three key stages: root cell dedifferentiation and division (14-18 DAC); meristem formation and development (25-35 DAC); root formation and elongation (50-55 DAC). The endogenous phytohormones play a key role during adventitious root development. For 25-5 and 23-12, the level of endogenous phytohormones, especially the ABA content, has an important effect on rooting capacity. For the effects of IBA on 8-9, except lower ABA content, the temporary peak of IAA on the 1 DAC was important for initiating the rooting process. The ratio of (IAA + GA 3 + ZR)/ABA can be used as an indicator of rooting capacity. The anatomical and physiological data described here for semi-lignified cuttings of L. kaempferi ϫ L. olgensis clones improves our knowledge of adventitious roots development. Our study will also benefit future research on examining the molecular mechanisms of the adventitious roots development in hybrid larch.

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“…The fundamental concept in a micropropagation system revolves around acclimation, with the final stage of the culture process referred to as rooting (Ismail et al, 2011;Millán-Orozco et al, 2011). In order to thrive in natural settings, in vitro-generated plantlets need a robust root system that contributes to the soil's absorption of water and nutrients (Benková & Bielach, 2010).…”

Section: Introductionmentioning

confidence: 99%

Influence of medium and gelling agents concentration on in vitro rooting of Polygonum tinctorium

Choi,

Kim,

Seo

et al. 2024

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Polygonum tinctorium has long been employed in the medical and dye industries in many nations, particularly in Korea and Japan, for traditional fabric staining as a source of blue color. The plant tissue culture method has proven to be a rapid and sustainable approach for the regeneration of various plant species, particularly those with medicinal or ornamental value. In this study, we explored the in vitro root regeneration and growth of P. tinctorium in response to different growth media and gelling agents. Among the tested media, Schenk and Hildebrandt medium (SH medium) demonstrated superior performance, resulting in the highest number and length of roots per explant. SH media produced about 53 and 18% higher root per explant and a 40% and 14% increase in root length compared to B5 and MS media, respectively. Half strength of SH medium proved to be the optimal condition for both root number (7.46 roots per explant) and root length (3.81 mm). Among the gelling agents, 2 g/L of Gelrite medium was most effective in promoting the highest and tallest roots. These findings have the potential to enhance rooting abilities in various crops, particularly in the case of medicinal and ornamental plants, and may offer valuable insights for future industrial-scale root production of P. tinctorium.

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In vitro root induction of faba bean ( Vicia faba L.)

Cited by 9 publications

References 0 publications

Enhancement of in Vitro Rooting By Gelling Agents and Activated Charcoal in <i>Rehmannia Glutinosa</i> L.

Enhancement of in Vitro Rooting By Gelling Agents and Activated Charcoal in <i>Rehmannia Glutinosa</i> L.

Anatomical and Physiological Effects of Phytohormones on Adventitious Roots Development in Larix kaempferi × L. olgensis

Influence of medium and gelling agents concentration on in vitro rooting of Polygonum tinctorium

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