In Vitro Rooting and Greenhouse Acclimatization of Lachenalia Shoots

Ault, James R.

doi:10.21273/hortsci.30.6.1304

Cited by 7 publications

(2 citation statements)

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“…Adventitious buds form on leaves (Klesser and Nel, 1976;Nel, 1983;Niederwieser and Vcelar, 1990;Niederwieser and Van Staden, 1990 a & b;Perrignon, 1992;Ault, 1995;Suh, Roh and Lee, 1997) bulb-scales, peduncles (unpublished) and axial meristems (Roodbol and Niederwieser, 1998).…”

Section: Origin Of Adventitious Budsmentioning

confidence: 99%

Review on Adventitious Bud Formation in Lachenalia

Niederwieser¹,

Ndou²

2002

Acta Hortic.

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Lachenalia varieties are propagated vegetatively through adventitious bud formation. In this report, published and unpublished results and observations are reviewed. Adventitious buds are formed on leaves, peduncles, bulb-scales and axillary meristems. In leaf tissue, buds form from epidermal cells and more than 90% originate from single cells. The regeneration potential of species and varieties varies amongst species and this factor thus became an important evaluation criterion in the breeding programme. Tissue age affects both the number of buds formed, as well as the size of bud/bulblet. Young tissue proved to be the best source of tissue in several studies. The physiological stage of the mother plant affected bud formation on bud forming tissue. Once plants enter the post anthesis stage, the regeneration potential decreases markedly. This effect is less evident when plant growth regulators (PGR's) are used in vitro. PGR's stimulate bud formation markedly and an interaction between the regeneration potential, tissue age and the optimal PGR concentration was shown. Propagation through adventitious bud formation will continue to play an important role in the production of the crop as lachenalias are not resistant to virus diseases. Growers therefore have to adhere to a propagation scheme with different phases and limited generations in each phase.

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Section: Origin Of Adventitious Budsmentioning

confidence: 99%

Review on Adventitious Bud Formation in Lachenalia

Niederwieser¹,

Ndou²

2002

Acta Hortic.

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“…Therefore, it is necessary to look for other, more efficient techniques than traditional methods of propagation. In vitro methods, such as organogenesis, allow for mass plant propagation and were described for selected lachenalia species and cultivars [6][7][8][9][10][11][12][13]. Most often the leaf tissue [11,[13][14][15][16][17] and less frequently bulb scales [12,18,19] were used as initial plant material to obtain adventitious bud formation.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Organogenesis of Critically Endangered Lachenalia viridiflora

2022

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Micropropagation via organogenesis of Lachenalia viridiflora W. F. Barker, a native endangered plant from South Africa, is presented in this study. Leaves, collected from in vivo plants, were a source for culture initiation to obtain three types of explants (leaf, bulb, callus). They were grown on Murashige and Skoog (MS) medium supplemented with two concentrations of sucrose (3 or 6%, marked as S3 and S6) and different concentrations of benzylaminopurine (BA) (0.0–5.0 μM) and naphthalene acetic acid (NAA) (0.0–0.5 μM). The results demonstrated that bulbing was more efficient on media with 3% sucrose and this process was closely related to explant type—irrespective of media type twice more adventitious bulbs (5.1–5.6) were regenerated on leaf and bulb explants than on callus (2.6). The highest value of adventitious bulb number was recorded for interaction: leaf × S3/2.5 BA/0.5 NAA (9.3 bulbs per explant). Irrespective of media type, the higher the number of adventitious bulbs (obtained from leaf and bulb explants), the lower their diameter. Moreover, adventitious bulbs regenerated from bulb explants formed the longest leaves (18.9 mm) and in the greatest percentage (52.3%). The highest biomass weight increase indicator (50 times) was recorded for interaction: leaf × S6/2.5 BA/0.25 NAA. Various combinations of plant growth regulators favor biomass increase.

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