In vitro safety assessment of electrohydrodynamically encapsulated Lactiplantibacillus plantarum CRD7 and Lacticaseibacillus rhamnosus CRD11 for probiotics use

Varada, Vinay Venkatesh; Panneerselvam, Divya; Pushpadass, Heartwin A.; Mallappa, Rashmi Hogarehalli; Chand, Ram; Kumar, Sachin

doi:10.1016/j.crfs.2023.100507

Cited by 8 publications

(1 citation statement)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because D-lactate production by L. paracasei IDCC 3401 was significantly lower than L-lactate production, L. paracasei IDCC 3401 exhibited positive metabolic properties associated with the production of toxic substances. In addition, L. plantarum CRD7 and L. rhamnosus CRD11 produced more D- lactic acid (0.65 ± 0.13 g/L and 0.70 ± 0.38 g/L, respectively) than L. paracasei IDCC 3401 (0.41 ± 0.05 g/L) [ 45 ]. Single-dose acute oral toxicity tests in rats can provide physiologic information as evidence of safety for human consumption [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, and Safety Evaluation of the Novel Probiotic Strain Lacticaseibacillus paracasei IDCC 3401 via Genomic and Phenotypic Approaches

Lee,

Bang,

Shin

et al. 2023

Microorganisms

View full text Add to dashboard Cite

This study aimed to explore the safety and properties of Lacticaseibacillus paracasei IDCC 3401 as a novel probiotic strain via genomic and phenotypic analyses. In whole-genome sequencing, the genes associated with antibiotic resistance and virulence were not detected in this strain. The minimum inhibitory concentration test revealed that L. paracasei IDCC 3401 was susceptible to all the antibiotics tested, except for kanamycin. Furthermore, the strain did not produce toxigenic compounds, such as biogenic amines and D-lactate, nor did it exhibit significant toxicity in a single-dose acute oral toxicity test in rats. Phenotypic characterization of carbohydrate utilization and enzymatic activities indicated that L. paracasei IDCC 3401 can utilize various nutrients, allowing it to grow in deficient conditions and produce health-promoting metabolites. The presence of L. paracasei IDCC 3401 supernatants significantly inhibited the growth of enteric pathogens (p < 0.05). In addition, the adhesion ability of L. paracasei IDCC 3401 to intestinal epithelial cells was found to be as superior as that of Lacticaseibacillus rhamnosus GG. These results suggest that L. paracasei IDCC 3401 is safe for consumption and provides health benefits to the host.

show abstract

Section: Discussionmentioning

confidence: 99%