In vitro schistosomicidal effects of some phloroglucinol derivatives from Dryopteris species against Schistosoma mansoni adult worms

Abstract

The rhizomes of Dryopteris species have popularly been used as vermifuge in flatworm infections. The aim of this work was to evaluate the in vitro schistosomicidal activity of some phloroglucinol compounds, obtained from the rhizomes of Dryopteris species, against Schistosoma mansoni adult worms. All worm pairs were dead after 24 h of incubation with aspidin 25 to 100 microM (1), flavaspidic acid 50 and 100 microM (2), methylene-bis-aspidinol 100 microM (3), and desaspidin 25 to 100 microM (4). Worms incubated with 1 (25 to 100 microM) and 2 (50 to 100 microM) showed decrease motor activity with tegumental alterations, while 3 (100 microM) and 4 (10 to 100 microM) showed decrease motor activity without tegumental alterations. Desaspidinol (5) and filicinic acid (6), at the tested concentrations (10 to 100 microM), did not show activity against adult worms of S. mansoni. Praziquantel (10 microM), used as positive control, caused death of the parasites and tegumental alterations without separation of worms. In the groups treated with 100 microM of compounds 1-4, the viability of the adult worms was similar to the positive control group, in which the worms were dead. Also, both the egg productions and the development of eggs produced by the adult worms were inhibited by the incubation with compounds 1-4 (10 and 100 microM) in comparison with the negative control (RPMI 1640 medium). It is suggested that the in vitro schistosomicidal effects of phloroglucinols derivatives 1, 2, 3, and 4 may be related to the inhibition of oxidative phosphorylation pathway in S. mansoni. The present results confirmed the traditional indications of rhizomes from Dryopteris species, which possess phloroglucinol compounds, in the treatment of tapeworm infections.

Evaluation of the in vitro Activity of Dermaseptin 01, a Cationic Antimicrobial Peptide, against Schistosoma mansoni

“…For example, curcumin, extracted from the rhizome of Curcuma longa [37], some phloroglucinol derivatives obtained from the rhizomes of Dryopteris species [38], and artenusate [36] presented in vitro inhibitory effects on schistosome oviposition. A decrease of the egg output of schistosomes was also observed for Nigella sativa crushed seeds (black seeds) [39] and an extract of ginger rhizomes (Zingiber officinale) [33].…”

In vitro schistosomicidal effects of the essential oil of Tagetes erecta

“…It is one of the most prevalent parasitosis in the world, second behind malaria. The World Health Organization (WHO) estimates that approximately 200 million people are currently contaminated, and that 800 million are at risk of contracting this disease (Magalhães et al, 2010;Steinmann et al, 2006). Today, praziquantel (PZQ) is the most widely employed drug for the treatment of Schistosomiasis, and it plays a key role in populationbased disease-control programs .…”

“…The search for antiparasitic compounds from natural sources has increased over the last decade, and plants continue to be a major source of biologically active compounds that may provide lead structures for the development of new drugs (Magalhães et al, 2010;Pontin et al, 2008). In this scenario, some essential oils have been recently pointed out as a promising alternative against Schistosoma mansoni Caixeta et al, 2011;Parreira et al, 2010).…”

Protective effect of Dryopteris crassirhizoma extracts in the control of the root-knot nematode Meloidogyne incognita

“…The phloroglucinol derivatives isolated from Mallotus japonicus (Euphorbiaceae) caused cytotoxicity in HeLa cells and inhibited the replication of herpes simplex virus type 1 (HSV-1) (Arisawa et al 1990). Furthermore, some phloroglucinol compounds such as aspidin, flavaspidic acid, methylene-bis-aspidinol and desaspidin from the rhizomes of Dryopteris species showed the in vitro schistosomicidal activity against killed Schistosoma mansoni adult worms within 24 hrs of incubation (Magalhães et al 2010). Specimen of S. mansoni incubated in aspidin and flavaspidic acid showed reduced motor activity with tegumental alterations, while those specimens incubated in methylene-bis-aspidinol and desaspidin showed reduced motor activity without tegumental alterations.…”