In vitro screening for Botrytis leaf blight resistance in Lilium species

Jang, Ji-Young; Subburaj, Saminathan; Lee, Geung–Joo; Kim, Hyun-Soon

doi:10.1016/j.scienta.2018.05.009

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…Subsequent ITS gene sequencing and phylogenetic analysis verified that the fungal strain detected was B. cinerea . Although B. elliptica has also been reported to cause gray blight in Lilium [ 7 ], this study did not identify B. elliptica in Bangladesh.…”

Section: Discussionmentioning

confidence: 72%

“…However, a disease infection that affects the flower production and quality has been subsequently reported. It is known that the gray mold caused by Botrytis elliptica and B. cinerea are major constraint for lilies production [ 7 ]. This disease could destroy 10–15 % of the plants of a farm after severe infection, resulting in serious loss to the lily producers [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Botrytis gray mold of Lilium in Bangladesh: Diagnosis, basic study and control

Alam,

Momotaz

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Botrytis gray mold of Lilium in Bangladesh: Diagnosis, basic study and control

Alam,

Momotaz

et al. 2024

Heliyon

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“…Published studies by Doss et al (1986) reported a large variation in disease incidence (25–98%) and lesion size among 35 cultivars, of which the hybrid type was in most cases undescribed. A more recent study by Jang et al (2018) also reported large variation in susceptibility among cultivars from four hybrid types and seven Korean genotypes from distinct indigenous species, with Asiatic hybrid genotypes generally falling in the highly resistant group, and the Korean genotypes generally falling in the susceptible group. A study by Gao et al (2018) , however, classified Oriental × Trumpet and Oriental hybrid cultivars as resistant, while Asiatic and Trumpet hybrids were classified as susceptible.…”

Section: Introductionmentioning

confidence: 89%

“…These previous studies, however, show several limitations that hamper obtaining a clear overview of the variation in susceptibility in lily and the variation in virulence in the B. elliptica population. Several studies included a single fungal isolate ( Beers et al, 2005 ; Hu et al, 2017 ; Gao et al, 2018 ; Jang et al, 2018 ) and distinct inoculation methods. Sometimes agar plugs were used as inoculum ( Hu et al, 2017 ; Gao et al, 2018 ), while in another case, leaf tips were inoculated with fungal conidia ( Beers et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Fire Blight Susceptibility in Lilium spp. Correlates to Sensitivity to Botrytis elliptica Secreted Cell Death Inducing Compounds

et al. 2021

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Fire blight represents a widespread disease in Lilium spp. and is caused by the necrotrophic Ascomycete Botrytis elliptica. There are >100 Lilium species that fall into distinct phylogenetic groups and these have been used to generate the contemporary commercial genotypes. It is known among lily breeders and growers that different groups of lilies differ in susceptibility to fire blight, but the genetic basis and mechanisms of susceptibility to fire blight are unresolved. The aim of this study was to quantify differences in fire blight susceptibility between plant genotypes and differences in virulence between fungal isolates. To this end we inoculated, in four biological replicates over 2 years, a set of 12 B. elliptica isolates on a panel of 18 lily genotypes representing seven Lilium hybrid groups. A wide spectrum of variation in symptom severity was observed in different isolate-genotype combinations. There was a good correlation between the lesion diameters on leaves and flowers of the Lilium genotypes, although the flowers generally showed faster expanding lesions. It was earlier postulated that B. elliptica pathogenicity on lily is conferred by secreted proteins that induce programmed cell death in lily cells. We selected two aggressive isolates and one mild isolate and collected culture filtrate (CF) samples to compare the cell death inducing activity of their secreted compounds in lily. After leaf infiltration of the CFs, variation was observed in cell death responses between the diverse lilies. The severity of cell death responses upon infiltration of the fungal CF observed among the diverse Lilium hybrid groups correlated well to their fire blight susceptibility. These results support the hypothesis that susceptibility to fire blight in lily is mediated by their sensitivity to B. elliptica effector proteins in a quantitative manner. Cell death-inducing proteins may provide an attractive tool to predict fire blight susceptibility in lily breeding programs.

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“…Bulbs were stored in a refrigerator (4 • C) for 28 days, and then placed at the bottom of 15-cm deep pots filled with turf: vermiculite: perlite substrate (1:1:1, v/v/v). The pots containing the bulbs were placed in a greenhouse for 45 days at 25 • C under 12-h light/12-h dark photoperiod, with photosynthetic photon flux density of 240 µmol•m.2•s-1 (Jang et al, 2018).…”

Section: Plant Cultivation and Pathogen Inoculationmentioning

confidence: 99%

Metabolic and Transcriptomic Profiling of Lilium Leaves Infected With Botrytis elliptica Reveals Different Stages of Plant Defense Mechanisms

Chai

Zuo

et al. 2021

Front. Plant Sci.

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Botrytis elliptica, the causal agent of gray mold disease, poses a major threat to commercial Lilium production, limiting its ornamental value and yield. The molecular and metabolic regulation mechanisms of Lilium's defense response to B. elliptica infection have not been completely elucidated. Here, we performed transcriptomic and metabolomic analyses of B. elliptica resistant Lilium oriental hybrid “Sorbonne” to understand the molecular basis of gray mold disease resistance in gray mold disease. A total of 115 differentially accumulated metabolites (DAMs) were detected by comparing the different temporal stages of pathogen infection. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed the differentially expressed genes (DEGs) and DAMs were enriched in the phenylpropanoid and flavonoid pathways at all stages of infection, demonstrating the prominence of these pathways in the defense response of “Sorbonne” to B. elliptica. Network analysis revealed high interconnectivity of the induced defense response. Furthermore, time-course analysis of the transcriptome and a weighted gene coexpression network analysis (WGCNA) led to the identification of a number of hub genes at different stages, revealing that jasmonic acid (JA), salicylic acid (SA), brassinolide (BR), and calcium ions (Ca2+) play a crucial role in the response of “Sorbonne” to fungal infection. Our work provides a comprehensive perspective on the defense response of Lilium to B. elliptica infection, along with a potential transcriptional regulatory network underlying the defense response, thereby offering gene candidates for resistance breeding and metabolic engineering of Lilium.

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In vitro screening for Botrytis leaf blight resistance in Lilium species

Cited by 5 publications

References 49 publications

Botrytis gray mold of Lilium in Bangladesh: Diagnosis, basic study and control

Botrytis gray mold of Lilium in Bangladesh: Diagnosis, basic study and control

Fire Blight Susceptibility in Lilium spp. Correlates to Sensitivity to Botrytis elliptica Secreted Cell Death Inducing Compounds

Metabolic and Transcriptomic Profiling of Lilium Leaves Infected With Botrytis elliptica Reveals Different Stages of Plant Defense Mechanisms

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