In vitro screening for salinity tolerance in pomegranate (Punica granatum L.) by morphological and molecular characterization

El-Mahdy, Marwa T.; Youssef, Muhammad; Elazab, Doaa S.

doi:10.1007/s11738-022-03361-2

Cited by 12 publications

(5 citation statements)

References 58 publications

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“…This difference between the two molecular markers might be due to the difference in their basis and regions they target in the genome. The results of the current study agreed with the previous Morsy et al, 2022 researches who reported the efficiency of ISSR in detecting DNA damages due to toxic metals and other mutagenic causes in many plant species e.g., , Eruca sativa (Al-Qurainy, 2010), Viola tricolor (Słomka et al, 2011), Solanum nigrum (Al Khateeb and Al-Qwasemeh, 2014), Sphagnum palustre (Sorrentino et al, 2017), Musa acuminata (Pisam et al, 2020;El-Mahdy et al, 2021) and Punica granatum (El-Mahdy et al, 2022).…”

Section: Discussionsupporting

confidence: 92%

“…Molecular analysis using simple and efficient tools such as ISSR and SCoT offers a good understanding and explaining for morphological and physiological observations. The two markers utilized in the current study (ISSR and/or SCoT) have been used previously in several studies, e.g., evaluation of genetic diversity (Youssef and Ibrahim, 2015;Khalil et al, 2020), abiotic stress screening (Abouzaid et al, 2016;El-Mahdy et al, 2021;El-Mahdy et al, 2022), mutation detection (Al-Qurainy, 2010) and assessment of heavy metals toxicity (Pisam et al, 2020). In the present study, ISSR and SCoT exposed their efficiency by confirming the observations at morphology and physiology levels.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Chromium harmful effects on in vitro banana performance

Youssef

Elsherbini

Morsy

2022

AJAS

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Abiotic stresses have a significant negative impact on plant growth and production. Among which, toxicity of heavy metals is considered one of the most factors affecting plant growth and agriculture on new reclaimed lands. In this study, the harmful effect of chromium was evaluated on in vitro banana plants of Grand Nain and Williams-Zeef cultivars based on morphological, physiological, and molecular assessments. Results showed that chromium significantly decreased all studied traits, including plant fresh weight, plant length, number of shoots per explant, and photosynthesis related pigments (chlorophyll and carotenoid content). The percentage of reduction due to chromium treatment ranged from 36.48 to 79.69% in shoot length of Grand Nain and chlorophyll-b in Williams-Zeef, respectively. Both banana cultivars were negatively affected by chromium treatment. However, Williams-Zeef showed higher reduction than that of Grand Nain in all studied traits. On the other hand, molecular analysis was performed to detect any variation between chromium-treated and untreated plants using inter simple sequence repeats (ISSR) and start codon targeted (SCoT) polymorphism. Results of molecular analysis confirmed the morphophysiological findings, by detection some polymorphic bands due to chromium treatment. In this regard, a total of six polymorphic bands were detected in the two banana cultivars, discriminating treated and control plants. In agreement with morphophysiological results, Williams-Zeef showed more polymorphism (five bands) due to chromium treatment than Grand Nain (one band). The screening protocol used in this study was efficient and helpful and could be used in successive studies to evaluate other toxicants and with other plant species as well.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Evaluation of Chromium harmful effects on in vitro banana performance

Youssef

Elsherbini

Morsy

2022

AJAS

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“…Similar to the number and length of shoots, 'Brigitta Blue' was the most affected, with FW decreasing by 106 times in the medium with 150 mM NaCl compared to the control. Consistent with these findings, various studies have reported a decrease in FW in different species subjected to salinity stress in vitro (Hannachi et al, 2021;El-Mahdy et al, 2022;Radi et al, 2023). Additionally, the negative effect of salinity on FW has been demonstrated through numerous in vivo studies on tomatoes with 75 mM NaCl concentration (Win et al, 2018) on okra seed-lings at mM 100 NaCl concentration (Wang et al, 2019) at apple rootstock (Matsumoto and Kobayashi, 2020) and sour cherry at 60 mM NaCl (Papadakis et al, 2018).…”

Section: Discussion Discussion Discussionsupporting

confidence: 53%

Investigation of salinity tolerance to different cultivars of highbush blueberry (Vaccinium corymbosum L.) grown in vitro

MOLNAR,

CLAPA,

POP

et al. 2024

Not Bot Horti Agrobo

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Salinity is one of the most critical abiotic stresses affecting various physiological, biochemical, and molecular functions of plants. This study aimed to assess the effects of different salt concentrations on in vitro blueberry shoots (‘Bluecrop’, ‘Blueray’, ‘Brigitta Blue’, ‘Duke’, ‘Goldtraube’, ‘Hortblue Petite’, and ‘Patriot’ cultivars) and to understand the mechanisms employed by this species under saline conditions. The Woody Plant Medium (WPM) proliferation medium was supplemented with 10, 50, 100, and 150 mM NaCl to induce salt stress. After ten weeks of in vitro culture under salinity stress, various parameters were evaluated, including the number of shoots obtained/explant, shoot length, fresh weight, dry weight, water content, stress tolerance index (STI), and McKinney index (MKI). Additionally, the behaviour of blueberry cultivars under salt stress was analyzed using electron paramagnetic resonance spectroscopy (EPR). Compared to the control (culture medium without NaCl), all treatments with NaCl reduced shoot length and the number of shoots obtained/explant in all studied blueberry cultivars. ‘Brigitta Blue’ reported the shortest shoots (0.04 ± 0.02 cm) at a concentration of 150 mM NaCl, followed by ‘Blueray’ with 0.05 ± 0.03 cm. Also, the lowest number of shoots/explant was recorded for both cultivars under 150 mM NaCl, namely 0.12 ± 0.07 shoots/explant (‘Brigitta Blue’) and 0.11 ± 0.04 shoots/explant (‘Blueray’). Salt tolerance, as expressed by ITS and MKI, confirmed that ‘Goldtraube’ exhibited higher salt tolerance, with the highest ITS values and the lowest MKI values. Further validation presented ‘Goldtraube’ as the most unresolved spectra of the Mn (II) hyperfine structure under all salt concentrations, therefore ‘Goldtraube’ was the most tolerant to saline stress.

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“…The most widespread visual evidence of heavy metal toxicity is a reduction in plant growth (Sharma and Dubey, 2007;Pisam et al, 2020;El Mahdy et al, 2021) including leaf chlorosis, necrosis, turgor loss, a decrease in the rate of seed germination, and a crippled photosynthetic apparatus, often correlated with progressing senescence processes or with plant death (Carrier, 2003). All these effects are related to ultrastructural, biochemical, and molecular changes in plant tissues and cells brought about by the presence of heavy metals (Gamalero, 2009;El-Mahdy et al, 2022).…”

Section: Number Of Shoots Per Explantmentioning

confidence: 99%

Establishment of an in vitro Protocol for Chromium Toxicity Tolerance Screening in Banana

Abdelhafiz

Elsherbini

Youssef

2022

Journal of Sohag Agriscience (JSAS)

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Several factors negatively affecting plant growth and production. Abiotic stresses play a vital role against many plant species. Among various abiotic stresses, heavy metals provide one of the most harmful effects on plant performance. Thus, this study was designed to establish an efficient in vitro protocol for chromium (Cr) toxicity tolerance screening in banana. One of the most important commercial banana cultivars (i.e., Grand Nain) was used. Five different concentrations of potassium dichromate (i.e., 50, 100, 200, 400 and 600 ppm) were tested along with free chromium medium as a control. Analysis of variance showed highly significant differences among Cr concentrations, affecting shoot length and number of shoots per explants, compared to control. In addition, results clearly showed the harmful effects of chromium on both shoot length and number of shoots per explant. In this regard, the percentage of reduction in shoot length due to Cr treatment ranged from 5.77 to 84.09% regarding treatment with 50 and 600ppm, respectively. Moreover, the number of shoots per explant was decreased by a range of 6.00 to 86.48% caused by treatment of 50 and 600ppm, respectively. According to these results and the analysis of variance, it is recommended to use the concentration of 400ppm potassium dichromate for in vitro screening of chromium tolerance in banana. The established protocol is efficient and of great importance and could be used for selecting chromium-tolerant genotypes in banana and other species.

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In vitro screening for salinity tolerance in pomegranate (Punica granatum L.) by morphological and molecular characterization

Cited by 12 publications

References 58 publications

Evaluation of Chromium harmful effects on in vitro banana performance

Evaluation of Chromium harmful effects on in vitro banana performance

Investigation of salinity tolerance to different cultivars of highbush blueberry (Vaccinium corymbosum L.) grown in vitro

Establishment of an in vitro Protocol for Chromium Toxicity Tolerance Screening in Banana

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