In Vitro Screening of Antioxidant and Antimicrobial Activities of Medicinal Plants Growing in Slovakia

Abstract

Traditional medicine usage at the preventive medicine level is comprehensive and plant-based remedies are being prescribed for the treatment of a large scale of diseases by indigenous medical practitioners around the world. The phytochemicals being observed in the fruits and vegetables as well as herbal plants are getting investigated intensively as their important role in the avoidance of diverse human diseases is undoubtful. Antioxidant and antimicrobial activities of selected medicinal herbs were evaluated in this study. Aerial parts of plants from Hypericaceae, Lamiaceae, Fabaceae, Plantaginaceae, Rubiaceae growing wild in Slovakia (locality Zobor) were investigated to characterize their polyphenol content, flavonoid content, as well as antioxidant and antimicrobial activity. Inspection of the metabolic profile combined with HPLC data showed that the ethanol extract contained mainly eight polyphenols with apigenin, daidzein, kaempferol, rutin, quercetin, vitexin, and cynaroside as the main chemicals. Among them, neochlorogenic acid, chlorogenic acid, trans-p-caffeic acid, trans-p-coumaric acid, trans-p-sinapic acid, trans-p-ferulic acid, rosmarinic acid were identified. The antioxidant capacity of ethanol extracts was evaluated by employing DPPH radical scavenging and phosphomolybdenum assays. All samples were also tested against Gram-positive (Staphylococcus aureus, Bacillus thuringiensis, Micrococcus luteus, Staphyloccocus epidermidis) and Gram-negative (Citrobacter koseri, Escherichia coli, Pseudomonas proteolytica, Hafnia alvei, Salmonella enterica, Yersinia enterocolitica) bacterial species using the disk diffusion and minimum inhibitory concentration assays

Melissa officinalis: Composition, Pharmacological Effects and Derived Release Systems—A Review

“…In a study of seven medicinal plants (from five plant families) grown in Slovakia, Gayibov et al [ 30 ] investigated the content of polyphenols and the content of flavonoids, as well as the antioxidant activity and antimicrobial activity. This study included three plants of the Lamiaceae family: Melissa officinalis , Salvia officinalis and Thymus pannonicus .…”

Content of phenolic compounds in leaves of trees growing on devastated lands at iron waste rock dump

“…Ці метаболіти зустрічаються в усіх органах рослини, але найбільша їх концентрація спостерігається в листках, квітках, нестиглих плодах. Фенольні сполуки виконують широкий спектр адаптивних функцій у рослин: 1) нейтралізують дію вільних радикалів; 2) беруть участь у захисті організму від захворювань (інгібують розмноження патогенних бактерій, вірусів і грибів); 3) зумовлюють наявність алелопатичних ефектів [12,26,31,33,37]. Відомо, що феноли також беруть участь у забезпеченні стійкості рослин до екстремальних умов зростання [7,9,22,28,31,33].…”

“…Уміст фенольних сполук у листках деревних видів рослин, що зростають у природних умовах, неодноразово був предметом наукових досліджень і темою наукових публікацій. В основному це результати дослідження біологічно активних речовин рослинного походження як перспективної сировини для фармакології, косметології та харчової промисловості [21,24,30,31,40].…”

<em>Aésculus hippocástanum</em>: Phytoscreening, Antiradical Activity, and Anti-Inflammatory Effect

“…Extracts were prepared from horse chestnut fruits by extraction of crushed raw materials with hot water (1:10), after which the resulting aqueous extracts were evaporated in vacuum to 1/10 of the initial volume and the extract complex was precipitated with a 3-fold amount of 95% alcohol. The antiradical activity was determined by standard protocol of measuring absorbance kinetics of free stable radical 2,2-diphenyl-1picrylhydrazyl (DPPH) as described elsewhere (Gayibova et al, 2019). Qualitative chemical composition was determined according to generally accepted methods (Bandiola et al, 2018).…”