In vitro screening of forty medicinal plant extracts from the United States Northern Great Plains for anthelmintic activity against Haemonchus contortus

Acharya, Jyotsna; Hildreth, Michael B.; Reese, R. Neil

doi:10.1016/j.vetpar.2014.01.008

Cited by 27 publications

(20 citation statements)

References 41 publications

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“…Pero en general, los valores de CL 50 de los extractos de P. sericea (20.36 mg mL −1 ) y A. tridentata (27.18 mg mL −1 ) son superiores en comparación con otros estudios que evalúan la actividad larvicida de extractos de plantas sobre H. contortus (Zhu et al 2013, Acharya et al 2014, Akkari et al 2014. La diferencia en ecacia que exhibieron P. sericea y A. tridentata en comparación con otros estudios realizados para evaluar la actividad ovicida y larvicida sobre H. contortus puede estar relacionada con el disolvente utilizado para la preparación de los extractos.…”

Section: Resultados Y Discusiónunclassified

“…Por ejemplo, los extractos acetónicos presentan más compuestos bioactivos de amplio rango de polaridad (Elo 1998) y exhiben mayor actividad ovicida y larvicida sobre H. contortus que los extractos acuosos (Adamu et al 2013, Fouche et al 2016. En muchas investigaciones se ha utilizado dimetil sulfóxido, compuesto que tiene capacidad emulsionante y solubiliza compuestos altamente polares o no polares, facilitando el paso de los compuestos a través del corión del huevo y la cutícula de la larva, lo que incrementa el porcentaje de inhibición de eclosión del huevo y mortalidad larval de H. contortus (Acharya et al 2014). El no emplear solvente químico, puede explicar la diferencia en ecacia que presentaron los extractos acuosos de P. sericea y A. tridentata.…”

Section: Resultados Y Discusiónunclassified

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Actividad ovicida y larvicida de extractos acuosos de Pluchea sericea y Artemisia tridentata en Haemonchus contortus

Montero¹,

Reyes²,

Aíl-Catzim³

et al. 2018

Ecosist. Recur. Agropec.

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El objetivo fue evaluar la actividad ovicida y larvicida de extractos acuosos de hojas de Pluchea sericea y Artemisia tridentata sobre Haemonchus contortus. Se evaluaron concentraciones de 100, 75, 50, 25 y 12.5 mg mL-1 de cada extracto. Se tuvo diferencias signicativas (p 0.05) para todas las concentraciones en la inhibición de la eclosión de huevos y mortalidad larval de H. contortus. La dosis de 100 mg mL-1 redujo la eclosión larvaria en 100 y 96.30%, con mortalidad larvaria del 92.67 y 89.33% para P. sericea y A. tridentata, respectivamente. La CL50 fue de 23.21 y 23.26 mg mL-1 para la inhibición de eclosión de huevos, para la mortalidad larvaria se tuvo una CL50 de 20.36 y 27.18 mg mL-1 para los extractos de P. sericea y A.tridentata, respectivamente. Los extractos acuosos de hojas de P. sericea y A. tridentata controlan los estadios de huevo y larva de H. contortus.

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Section: Resultados Y Discusiónunclassified

Actividad ovicida y larvicida de extractos acuosos de Pluchea sericea y Artemisia tridentata en Haemonchus contortus

Montero¹,

Reyes²,

Aíl-Catzim³

et al. 2018

Ecosist. Recur. Agropec.

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“…The anthelmintic potential of plant extracts have been evaluated in a preliminary in vitro assay with H. contortus (Acharya et al, 2014). These tests, although less accurate than in vivo tests, are faster, cheaper and easier to be reproduced (Qi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and in vitro activity of Calotropis procera (Ait.) latex on Haemonchus contortus

Cavalcante

Morais

André

et al. 2016

Veterinary Parasitology

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“…Sheep gastrointestinal nematodes are thought to be a major health problem faced by these animals around the world, and infections caused by these parasites are responsible for great losses in sheep raising (Acharya et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

et al. 2021

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Gastrointestinal nematodes are responsible for great economic losses in sheep raising, and their control has long been carried out almost exclusively by the administration of anthelmintics, which have led to serious resistance problems. In the search for alternative control measures, phytotherapic research is highlighted. The aim of this study was to evaluate the action of Anethum graveolens (dill) essential oil on different stages of Haemonchus contortus life cycle, as well its cytotoxicity MDBK (Madin-Darby bovine kidney) cells. H. contortus larvae and eggs were obtained from infected sheep feces, and essential oil extracted from plant seeds through the Clevenger apparatus. 9.4, 4.7, 2.35, 1.17. 0.58 and 0.29 mg/mL concentrations were evaluated. The Egg Hatch Inhibition (HI), Larval Development Inhibition (LDI) and Larval Migration Inhibition (LMI) techniques were used. Thybendazole 0.025 mg/mL in HI and Levamisole 0.02 mg/mL in the LDI and LMI tests were used as positive controls, while distilled water and a Tween 80 solution were used as positive negative controls. The inhibition results obtained for the highest oil concentration were: HI 100%, LDI 98.58% and LMI 63.7%, differing ( <0.05) from negative controls. Main A. graveolens oil components present in 95.93% of the total oil were Dihydrocarvone (39.1%), Carvone (22.24%), D-Limonene (16.84%), Apiol (10.49%) and Trans-dihydrocarvone (7.26%). Minimum A. graveolens essential oil concentrations required to inhibit 50% (IC50) of egg hatching, larval development and larval migration were 0.006 mg/mL, 2.536 mg/mL and 3.963 mg/mL, respectively. Cell viability in MDBK (Madin-Darby bovine kidney) cells, when incubated with A. graveolens essential oil, was 86% for the highest (9.4 mg/mL) and 99% for the lowest concentration (0.29 mg/mL). A. graveolens essential oil, according to the results obtained in this study, is a promising alternative in sheep gastrointestinal nematode control. ResumoOs nematoides gastrintestinais são responsáveis por grandes perdas econômicas na ovinocultura, e seu controle tem sido realizado quase exclusivamente pela administração de anti-helmínticos, que levaram a sérios problemas de resistência. Na busca de medidas alternativas de controle, destaca-se a pesquisa fitoterápica. O objetivo deste trabalho foi avaliar a ação do óleo essencial de Anethum graveolens (endro) em diferentes estágios de Haemonchus contortus, bem como testar a viabilidade celular para o óleo. Larvas e ovos de H. contortus foram obtidos de fezes de ovinos infectados e óleo essencial extraído de sementes de plantas através do aparelho de Clevenger. As concentrações avaliadas foram 9,4, 4,7, 2,35, 1,17, 0,58 e 0,29 mg/mL. Verificou-se a Inibição de eclosão dos ovos (IE), Inibição de Desenvolvimento Larval (IDL) e Inibição de Migração Larval (IML). Tiabendazol 0,025 mg/mL em IE e Levamisole 0.02 mg/mL nos testes IDL e IML foram usados como controles positivos, enquanto água destilada e uma solução Tween 80 foram usados como controles negativos. Os resultados de inibição o...

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In vitro screening of forty medicinal plant extracts from the United States Northern Great Plains for anthelmintic activity against Haemonchus contortus

Cited by 27 publications

References 41 publications

Actividad ovicida y larvicida de extractos acuosos de Pluchea sericea y Artemisia tridentata en Haemonchus contortus

Actividad ovicida y larvicida de extractos acuosos de Pluchea sericea y Artemisia tridentata en Haemonchus contortus

Chemical composition and in vitro activity of Calotropis procera (Ait.) latex on Haemonchus contortus

Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

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