In vitro screening of lactic acid bacteria isolated from gastrointestinal tract of Atlantic Salmon (Salmo salar) as probiont candidates

Amin, Muhamad; Adams, Mark B.; Bolch, Christopher J. S.; Burke, Christopher M.

doi:10.1007/s10499-016-0045-6

Cited by 26 publications

(19 citation statements)

References 30 publications

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“…However, the PCR amplicon sizes for KHV in the present study were also longer than previously published, including 484 bp [14], 409 bp by Bercovier et al [5], and 414 bp by Bergmann et al [6]. These differences might be due to the different primers which targeted different genes [2]. Based on on Primer-BlAST program, the primers used in the current study targeted 410 bp, located at 96,160 and 96,553.…”

Section: Discussioncontrasting

confidence: 51%

Detection of koi herpesvirus in healthy common carps, Cyprinus carpio L.

et al. 2018

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Koi herpesvirus (KHV), a member of Hervesviridae, has been frequently reported to cause mass mortality (80-100%) in common carps (Cyprinus carpio L.). A unique feature of Herverviridae members is latent infection, maintaining their genetic information for an extended period in the absence of productive infection, and reactivate when environmental conditions are favorable for their growth. To prevent this occurs, a monitoring program should be done for early detection. This study aimed at detecting the presence of KHV in healthy common carps reared in West-Nusa Tenggara Province, Indonesia. A total of 80 healthy fish was collected randomly from eight fish farms (Lingsar,

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Section: Discussioncontrasting

confidence: 51%

Detection of koi herpesvirus in healthy common carps, Cyprinus carpio L.

et al. 2018

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“…In this study, bacterial isolates were considered as closely associated with the intestinal wall (autochthonous), since faeces were removed and loosely associated, transient bacteria were washed away before detaching candidate bacteria with a non-ionic surfactant (Triton X 100), which is commonly used for this purpose [ 49 , 50 ]. The ability to attach to the mucosal surface, pH and the resistance to bile acids are particularly important to allow a successful colonization of the intestine [ 51 , 52 ]. Although there is evidence for a core gut microbiota in teleost fish, the diversity of identified intestinal bacteria is enormous and depending on various biotic and abiotic factors [ 48 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of native probiotics for fish farming

et al. 2018

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BackgroundInnovations in fish nutrition act as drivers for the sustainable development of the rapidly expanding aquaculture sector. Probiotic dietary supplements are able to improve health and nutrition of livestock, but respective bacteria have mainly been isolated from terrestrial, warm-blooded hosts, limiting an efficient application in fish. Native probiotics adapted to the gastrointestinal tract of the respective fish species will establish within the original host more efficiently.ResultsHere, 248 autochthonous isolates were cultured from the digestive system of three temperate flatfish species. Upon 16S rRNA gene sequencing of 195 isolates, 89.7% (n = 175) Gram-negatives belonging to the Alpha- (1.0%), Beta- (4.1%) and Gammaproteobacteria (84.6%) were identified. Candidate probiotics were further characterized using in vitro assays addressing 1) inhibition of pathogens, 2) degradation of plant derived anti-nutrient (saponin) and 3) the content of essential fatty acids (FA) and their precursors. Twelve isolates revealed an inhibition towards the common fish pathogen Tenacibaculum maritimum, seven were able to metabolize saponin as sole carbon and energy source and two isolates 012 Psychrobacter sp. and 047 Paracoccus sp. revealed remarkably high contents of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Furthermore, a rapid and cost-effective method to coat feed pellets revealed high viability of the supplemented probiotics over 54 d of storage at 4°C.ConclusionsHere, a strategy for the isolation and characterization of native probiotic candidates is presented that can easily be adapted to other farmed fish species. The simple coating procedure assures viability of probiotics and can thus be applied for the evaluation of probiotic candidates in the future.

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“…Besides having antagonistic activity against bacterial pathogens, probiotic candidates should be able to survive under gastrointestinal conditions (Geraylou et al, 2014;Amin et al, 2016). The result of our evaluation suggests that six LAB were able to survive in stomach conditions, although their viability slightly decreased except for L. lactis.…”

Section: Discussionmentioning

confidence: 85%

Isolation and screening of lactic acid bacteria producing anti-edwardsiella from the gastrointestinal tract of wild-caught catfish (Clarias gariepinus) for probiotic candidates

Nurhayati

Zulaika

Amin

et al. 2022

Preprint

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Members of lactic acid bacteria (LAB) have been well-known for their antagonistic activities against various bacterial pathogens infecting aquaculture species. Thus, isolation of LAB members from wild aquatic species and screening them for antimicrobial production have been intensively done in the last few decades to replace the indiscriminative use of antibiotics. The present study aimed at isolating LAB members from the intestinal tract of wild-caught catfish, Clarias gariepinus, and screening them for antimicrobial production against one of the most common bacterial pathogens, Edwardsiella ichtaluri. The result showed that a total of 29 LAB were successfully isolated and further screened for anti-edwarsiella activities. Of the 29, 6 isolates had strong anti-Edwardsiella activity (diameter of inhibition zone > 10mm). Based on their 16s rRNA gene sequences, these LABs were identified as Lactococcus lactis, Enterococcus hirae, Weissella confusa, Weissella cibaria, and Enterococcus faecalis (2 isolates). Further in vitro studies indicated that E. faecalis, L. lactis, E. confusa and E. cibaria showed the same viability except for E. hirae in the intestinal fluid simulation. All LAB had a good adhesion capacity to intestinal mucus between 3,42 − 16,72%. Four LABs were sensitive to erythromycin, oxytetracycline, and novobiocin, while E. faecalis and W. cibaria were resistant to novobiocin and enrofloxacin, respectively. In vivo assay also indicated that all six LABs were non-pathogenic to catfish. These results suggested that E. faecalis, L. lactis, and W. confusa are potential probiotic candidates in aquaculture to prevent enteric septicemia of catfish (ESC) disease. However, further studies are needed to evaluate the use of probiotics in vivo.

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In vitro screening of lactic acid bacteria isolated from gastrointestinal tract of Atlantic Salmon (Salmo salar) as probiont candidates

Cited by 26 publications

References 30 publications

Detection of koi herpesvirus in healthy common carps, Cyprinus carpio L.

Detection of koi herpesvirus in healthy common carps, Cyprinus carpio L.

Isolation and characterization of native probiotics for fish farming

Isolation and screening of lactic acid bacteria producing anti-edwardsiella from the gastrointestinal tract of wild-caught catfish (Clarias gariepinus) for probiotic candidates

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