In vitro screening of potato lines for drought tolerance

Al-Biski, F.; Najla, Safaa; Sanoubar, Rabab; Alkabani, Nour; Murshed, Ramzi

doi:10.1007/s12298-012-0127-5

Cited by 70 publications

(72 citation statements)

References 28 publications

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“…The osmotic potential of the medium (Table S2, liquid medium) was comparable with values obtained by Gopal and Iwama () and Albiski et al. (), if the contribution of the agar was subtracted. The effect of agar on the osmotic potential of MS medium was analyzed before (Gopal et al.…”

Section: Discussionsupporting

confidence: 86%

Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening: Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials

Bündig

Meise

et al. 2016

J Agronomy Crop Science

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Solanum tuberosum (potato) as a drought sensitive plant is also one of the most promising plants to meet the demands for food and starch of a growing population. Distinguishing genotypes into tolerant and susceptible is therefore of utmost interest. We subjected eighteen potato genotypes and two wild species, S. tarijense and S. chacoense, to osmotic stress applied in vitro by addition of 0.2 m sorbitol to a solid medium. Here, we report that a ratio of root:shoot dry mass (DM) together with the SSI (stress susceptibility index, equivalent to drought susceptibility index by Fischer and Maurer, Aust. J. Agron. Res., 29, 1978) of shoot DM were found to be relevant parameters to characterize genotypes in vitro for their osmotic stress tolerance. Drought stress data from pot trials in a rainout shelter (2013 and 2015) correlated poorly with the data obtained in in vitro experiments. However, the most tolerant and most sensitive genotypes in vitro were also categorized to be more tolerant or sensitive than the average to drought stress in vivo. Both, under in vitro and in vivo conditions, proline displayed an increase under osmotic stress conditions in nearly all potatoes tested, but no direct correlations were found to stress tolerance. However, a genotype classified as tolerant displayed earlier proline accumulation. Proline is thought of as one factor for plants to withstand stressful conditions, but cannot be used to distinguish potato genotypes for their stress tolerance to osmotic stress in vitro. Analysis of the osmotic potential of in vitro and in vivo stressed plants displayed a general increase compared to the control.

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Section: Discussionsupporting

confidence: 86%

Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening: Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials

Bündig

Meise

et al. 2016

J Agronomy Crop Science

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“…Plants under water stress have been reported to show a decrease in leaf conductance (Obidiegwu et al, 2015), total area of leaves (Albiski et al, 2012), reduced plant chlorophyll content (Anithakumari et al, 2012), and the photosynthesis rate (Li et al, 2015). Loggini et al (1999), and Apel and Hirt (2004) reported that drought inhibits or slows down photosynthetic carbon fixation mainly through limiting the entry of CO 2 into the leaf or directly inhibiting metabolism.…”

Section: Discussionmentioning

confidence: 99%

Improving Seed Potato Leaf Area Index, Stomatal Conductance and Chlorophyll Accumulation Efficiency through Irrigation Water, Nitrogen and Phosphorus Nutrient Management

Kingori¹,

Nyamori²,

Khasungu³

2016

JAS

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A study was conducted in a Rainshelter (RTrial) at Horticultural Research and Teaching Farm, Egerton University to determine the effect of integration of irrigation water, nitrogen (N) and phosphorus (P) application on seed potato leaf area index (LAI), stomatal conductance and chlorophyll content. The treatments arranged in a split-split plot layout in a completely randomised block design, consisted of three irrigation water rates (40%, 65% and 100% field capacity), four N rates (0, 75, 112.5 and 150 kg N/ha) supplied as urea (46% N), and four P rates (0, 50.6, 75.9, 101.2 kg P/ha) supplied as triple superphosphate, replicated three times and repeated once. During the growth leaf area, stomatal conductance, and chlorophyll content were measured. Data collected were subjected to analysis of variance and significantly different means separated using Tukey's Studentized Range Test at P≤0.05. Leaf area index was greater with high irrigation water at 100%, N at 150 kg N/ha and P at 101.2 kg P/ha, which was 2.6 and 1.3 at 51 days after planting (DAP) and 3.5 and 3.1 at 64 DAP. Furthermore, low irrigation water rate at 40% together with low N and P rates of 0 kg N/ha and 0 kg P/ha had the least LAI, which was 0.28 and 0.19 at 51 DAP and 0.28 and 0.24 at 64 DAP both in RTrials I and II, respectively. Subjecting potato to 100% compared to 40% 16.2 and 16.5, 19.8 and 19.6, and 15 and 20.3, when integrated with high compared with low N and P application rates at 59, 73 and 87 DAP, in RTrials I and II respectively. Irrespective of N and P rates LAI, stomatal conductance and chlorophyll content were significantly greater with high irrigation water at 100% followed by 65% and was lowest with 40% irrigation water rate.

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“…Potato (Solanum tuberosum L.) is one of the most important vegetables in the world (Albiski et al, 2012). Potato is very sensitive to water-stress because of its shallower root system (Iwama and Yamaguchi, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Also, under extreme water-stress conditions as induced by 0.4 M sorbitol, all genotypes had little shoot as well as root growth. Albiski et al (2012) reported that sorbitol inducing water stress in morphological parameters in potato. They mentioned that the drought induced by addition of sorbitol on MS medium led to decrease in the potato stem length, number of leaves and number of roots per plant according to potato genotypes and drought.…”

Section: Introductionmentioning

confidence: 99%

In vitro screening of potato (Solanum tuberosum L.) cultivars for drought stress tolerance.

Labeb

Zaki

Ati

et al. 2020

SVU-International Journal of Agricultural Sciences

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This study was carried out at the tissue culture lab, Horticulture Department, Faculty of Agriculture, Minia University, Minia, Egypt. under in vitro condition to screening the response of four potato varieties (Diamond; Burren; Maritiema and Lady Balfour) to water stress induced by adding sorbitol in the culture medium. Four concentrations of sorbitol (0.0, 0.1, 0.2 and 0.3 mole /liter), in Murashige-Skoog (MS) medium were used for exerting the water deficiency stress on the plantlets with three replications, in completely randomized design. Data revealed that the potato cultivars were significant differed for plantlet height, number of leaves per plantlet, number of nodes per plantlet, number of roots per plantlet and plantlet weight in the medium without sorbitol. Moreover, different response of potato cultivars was observed to various levels of sorbitol concentrations. Increasing the concentration of sorbitol lead to significant decreased in all studied traits, as compared with control treatment (sorbitol free). However, the most reduction of morphological parameters was observed in Maritiema and Lady Balfour cultivars, and considered as susceptible cultivars for water deficit. These results indicated also that the Diamond variety is a droughttolerant than the other potato cultivars which used in the current study. These results indicated that the simulation of drought stress under in vitro conditions during the regeneration process constitutes a convenient way to study the effects of drought on potato responses. In-vitro this technique was shown to be useful in identifying relatively droughttolerant genotypes at early stages of development and this can be a very useful tool for screening large number of varieties or breeding lines of genotypes within a short time.

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In vitro screening of potato lines for drought tolerance

Cited by 70 publications

References 28 publications

Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening: Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials

Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening: Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials

Improving Seed Potato Leaf Area Index, Stomatal Conductance and Chlorophyll Accumulation Efficiency through Irrigation Water, Nitrogen and Phosphorus Nutrient Management

In vitro screening of potato (Solanum tuberosum L.) cultivars for drought stress tolerance.

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