In Vitro Seed Germination and Plant Growth of “Cabeça-De-Frade” (Cactaceae)

Resende, Sheila Vitória; Lima-Brito, Alone; Torres-Silva, Gabriela; Santana, José Raniere Ferreira de

doi:10.1590/1983-21252021v34n101rc

Cited by 5 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For sweet cactus ( Nopalea cochenillifera ) species, the lowest contamination percentage was obtained when seeds were disinfected with 70% of ethanol for 1 min, followed by a deep soaking in a commercial bleach solution (sodium hypochlorite) at 1.5% for 10 min (Castro et al, 2011 ). Resende et al ( 2021 ) applied a higher concentration of ethanol (96%) and sodium hypochlorite (2%) for the surface sterilization of Melocactus glaucescens seeds. The surface sterilization was established using 0.1% of commercial detergent (Extran) before the disinfection with 70% of ethanol for 1 and 25 min soaking in sodium hypochlorite solution at 2% (Dávila-Figueroa et al, 2005 ).…”

Section: Constraints Of In Vitro Propagation In Cactimentioning

confidence: 99%

Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review

et al. 2022

View full text Add to dashboard Cite

Cacti are one of the most significant and diversified groups of angiosperms, distributed and cultivated globally, mostly in semi-arid, arid, and the Mediterranean climate regions. Conventionally, they are propagated by seeds or through vegetative propagation via rooted offshoots or grafting. However, these multiplication procedures remain insufficient for mass propagation. In vitro culture techniques are utilized to mass propagate endangered and commercial cacti species. These include somatic embryogenesis and plant regeneration through indirect or direct organogenesis. The latter is a promising tool for commercial clonal propagation of high-value species and has been successfully implemented for several species, such as Mammillaria, Hylocereus, Cereus, Echinocereus, and Ariocarpus. However, its success depends on explant type, basal nutrient formulation of culture medium, and types and concentrations of plant growth regulators. This study aimed to assess the potential of in vitro propagation methods applied to cacti species and discuss the different factors affecting the success of these methods. This study has also highlighted the insufficient work on Opuntia species for mass propagation through axillary buds' proliferation. The development of an efficient micropropagation protocol is thus needed to meet the supply of increasing demand of Opuntia species for human consumption as fruit, animal feed, and ecological restoration in semi-arid and arid zones.

show abstract

Section: Constraints Of In Vitro Propagation In Cactimentioning

confidence: 99%

Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Aside from analyzing the replacement of reagents for analysis by lower-cost options to reduce final seedling cost, the present study analyzed the efficacy of sterilization using sodium hypochlorite, as it has been reported to be effective in the Research, Society andDevelopment, v. 10, n. 4, e4811425143, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i4.25143 6 chemical sterilization of nutritious media instead of thermal sterilization by autoclaving (Pais et al, 2016;Cardoso & Imthurn, 2018;Conceição et al, 2021). Along with its efficacy, chemical sterilization has also proved to be cost-effective compared to autoclaving, as it represents the easiest and most viable technique for microbial decontamination (RESENDE et al, 2021).…”

Section: Gelling Agents Used In Chemical Sterilizationmentioning

confidence: 99%

Xanthan gum and sodium hypochlorite in vitro rooting of Gerbera hybrida cv. Essandre

Ribeiro

Teixeira

et al. 2022

RSD

View full text Add to dashboard Cite

The aim of this study was to check the use of the following gelling agents: the xanthan gum “Adicel®” and the stabilizer “Super Liga Neutra®” to replace agar in the in vitro rooting phase of Gerbera hybrida cv. Essandre. Additionally, the possibility of using chemical sterilization of both culture media and glassware with sodium hypochlorite to replace autoclaving was analyzed. The gelling agents, xanthan gum “Adicel®” and the stabilizer “Super Liga Neutra®” were tested at the following concentrations (g L-1): 7; 9; 11; 13; 15; 17; 19; and 21. No concentrations of Super Liga Neutra® provided effective solidification. Concentrations of 17 and 21 g of Adicel® provided a good gelling of the culture medium, which was compared to the medium containing agar (control) with two types of sterilization: autoclaving (for 20 and 40 minutes) and chemical sterilization. Autoclaving for 20 minutes did not provide effective elimination of contamination in the culture medium containing xanthan gum; this only occurred when autoclaving time increased to 40 minutes. Plant development in culture media containing 17 and 21 g of xanthan gum, either sterilized by autoclaving for 40 minutes or at a concentration of 17 g xanthan gum using sodium hypochlorite, was statistically the same as the control that contained agar. However, plant development at a concentration of 21g of xanthan gum in a sterilized medium using sodium hypochlorite was lower than that observed in media containing agar.

show abstract

“…Researches on other families of ornamental species found in the Chapada Diamantina Highland showed viability of in vitro cultivation for Bromeliaceae species (LIMA et al, 2012; LIMA; LIMA-BRITO; SANTANA, 2020), evergreen flowers (ALBUQUERQUE et al, 2016;LIMA-BRITO et al, 2016;LIMA-BRITO et al, 2011; LIMA; LIMA-BRITO; SANTANA, 2021; CARMO; MOURA; LIMA-BRITO, 2020), and Cactaceae species (RESENDE et al, 2021;TORRES-SILVA et al, 2021); however, studies on Velloziaceae species occurring in this region are needed.…”

Section: Introductionmentioning

confidence: 98%

Micropropagation of Vellozia seubertiana (Velloziaceae)

Pinto

Lima-Brito

2023

Rev. Caatinga

Self Cite

View full text Add to dashboard Cite

Velloziaceae is one of the main families in the floristic composition of the Campos Rupestres Montane Savanna ecoregion of the Chapada Diamantina Highland, Bahia, Brazil, and has species with significant ornamental potential and resistance to climate changes. The species Vellozia seubertiana stands out for its beautiful flowers and stems covered by leaf sheaths that ensure protection when in contact with fire. However, there is no information on its propagation, conservation, and physiology, which justifies this study. The objective of this work was to establish a micropropagation protocol for V. seubertiana. Seeds were disinfected and inoculated in Murashige and Skoog culture medium (MS) with half salt concentration (MS½) for in vitro establishment. Plantlets established in vitro were used to induce sprouting in MS½ supplemented with 6-benzylaminopurine (BAP; 0.00, 4.44, 8.88, and 17.76 µM) and 1-naphthaleneacetic acid (NAA; 0.00 and 2.22 µM). The shoots obtained were inoculated in MS½ containing activated charcoal (0.0 and 1.0 g L−¹) and indole-3-butyric acid (IBA; 0.00 and 2.22 µM) for rooting. The rooted plantlets were acclimated under greenhouse conditions. A mean of 5.7 shoots were generated through organogenesis in medium containing 8.75 µM of BAP and 2.22 µM of NAA. The use of activated charcoal resulted in the highest means for aerial part and root lengths in the in vivo rooting phase. Acclimated plants reached 75% survival at 60 days after transplanting to ex vitro conditions. The results indicate that micropropagation is a promising technique for the production of V. seubertiana seedlings.

show abstract

In Vitro Seed Germination and Plant Growth of “Cabeça-De-Frade” (Cactaceae)

Cited by 5 publications

References 15 publications

Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review

Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review

Xanthan gum and sodium hypochlorite in vitro rooting of Gerbera hybrida cv. Essandre

Micropropagation of Vellozia seubertiana (Velloziaceae)

Contact Info

Product

Resources

About