In vitro selection and characterization of deoxyribonucleic acid aptamers for digoxin

Kiani, Zahra; Shafiei, Massoumeh; Rahimi‐Moghaddam, Parvaneh; Karkhane, Ali Asghar; Ebrahimi, Soltan Ahmad

doi:10.1016/j.aca.2012.08.025

Cited by 31 publications

(26 citation statements)

References 35 publications

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“…42 The fabricated aptasensor could be considered as a simple system in comparison with most reported aptasensors because it does not need any prelabeling step. In addition, GNP/FTO is prepared through a simple and rapid one-step mixing procedure without need to any complicated and costly synthetic strategies.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

2015

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In this paper we used a modified fluorine-doped tin oxide (FTO) as the substrate of a novel aptasensor for electrochemical determination of digoxin. For this purpose, a selective thiolated digoxin aptamer was immobilized onto the gold nanoparticles deposited FTO (GNPs/FTO) surface. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the characterization of modified surfaces. The aptasensor response was based on decrease in the methylene blue (MB) current, as an electrochemical probe, after incubating with digoxin. Digoxin can be determined in linear concentration range from 0.02 to 0.2 µg/L by differential pulse voltammetry (DPV). The detection limit of the proposed aptasensor was 0.01 µg/L. Also, the fabricated aptasensor was applied to determine digoxin in urine and blood plasma samples with satisfactory results. Furthermore, high sensitivity and specificity, low detection limit and fast response, can make it possible to determine trace amounts of digoxin for routine clinical analysis.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

2015

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“…This is the key procedure for the SELEX method. The separation methods include the NC membrane method [44], affinity chromatography [45], biotin-streptavidin separation [46], and capillary electrophoresis [47] method. The sequences bound with the target are separated by alkali or heat denaturation and then amplified by PCR.…”

Section: Selex Methodsmentioning

confidence: 99%

Novel methodologies in analysis of small molecule biomarkers and living cells

Chen

Zhu

2014

Tumor Biol.

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Enzyme-linked immuno-sorbent assay (ELISA) is widely used for biomarker detection. A good biomarker can distinguish patients from healthy or benign diseases. However, the ELISA method is not suitable for small molecule or trace substance detection. Along with the development of new technologies, an increasing level of biomaterials, especially small molecules, will be identified as novel biomarkers. Quantitative immuno-PCR, chromatography-mass spectrometry, and nucleic acid aptamer are emerging methodologies for detection of small molecule biomarkers, even in living cells. In this review, we focus on these novel technologies and their potential for small molecule biomarkers and living cell analysis.

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“…The sensing mechanism is based on the target‐induced conformational change of the digoxin‐specific aptamer sequence . The aptamer used in this study is a 50‐base DNA oligonucleotide, which has been proven to have high affinity toward digoxin . One end of the aptamer is immobilized on a gold (Au) electrode surface and the other end of the aptamer is tagged with a methylene blue molecule, which is used as a redox label.…”

Section: Figurementioning

confidence: 99%

“…Figure presents the design of a signal‐on E‐AB digoxin sensor and proposed sensing mechanism. The aptamer used in this study is a 50‐base sequence that has been modified from studies reported by Ebrahimi and co‐workers . According to a prediction derived from Mfold (a web server that provides nucleic acid folding and hybridization predictions), a short portion of the aptamer is presumed to have a helix structure and hairpin loop.…”

Section: Figurementioning

confidence: 99%

“…[6] The aptamer used in this study is a 50-base DNA oligonucleotide, which has been proven to have high affinity toward digoxin. [7] One end of the aptamer is immobilized on a gold (Au) electrode surface and the other end of the aptamer is tagged with a methylene blue molecule, which is used as a redox label. After the surface-immobilized aptamer binds to a digoxin molecule, it brings the methylene blue closer to the electrode surface.…”

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confidence: 99%

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Direct Electrochemical Aptamer‐Based Detection of Digoxin

2020

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Here we report a signal‐on electrochemical aptamer‐based (E‐AB) sensor for the detection of digoxin at clinically relevant levels. Digoxin is a plant‐based medication that is prescribed to treat patients with congestive heart failure and chronic atrial fibrillation and atrial flutter. Digoxin has a narrow therapeutic range index of between 0.5 ng/mL and 2.0 ng/mL. The aptamer used in this study is a 50‐base DNA oligonucleotide that has been proven to have high affinity toward digoxin. One end of the aptamer is immobilized on a gold electrode surface and the other end is tagged with a methylene blue molecule, which is used as a redox label. Like other signal‐on E‐AB sensors, once the target binds to the aptamer‐modified electrode, the methylene blue reduction current increases. The developed E‐AB digoxin sensor provides a distinct and amplified peak current response in the therapeutic range of 0.5 ng/mL to 2.0 ng/mL in a phosphate buffer saline solution. The advantage of this detection method is that it allows an aptamer‐modified electrode to perform single‐step and reagentless sensing. We suggest this approach as a conceptual pathway to point‐of‐care E‐AB digoxin sensor design.

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In vitro selection and characterization of deoxyribonucleic acid aptamers for digoxin

Cited by 31 publications

References 35 publications

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

Novel methodologies in analysis of small molecule biomarkers and living cells

Direct Electrochemical Aptamer‐Based Detection of Digoxin

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