2013
DOI: 10.4161/adna.25786
|View full text |Cite
|
Sign up to set email alerts
|

In vitro selection of BNA (LNA) aptamers

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
28
0
1

Year Published

2015
2015
2024
2024

Publication Types

Select...
7
3

Relationship

0
10

Authors

Journals

citations
Cited by 61 publications
(29 citation statements)
references
References 96 publications
0
28
0
1
Order By: Relevance
“…Length of the random region of the starting library is normally between 20 to 40 bp [11]. Modified nucleotides could be also included in the library, which may greatly broaden the range of possible sequences and probably enhance their in vivo stability or nuclease resistance [24]. The design of the conserved primer regions is also important as improper design would introduce unspecific products in PCR.…”
Section: Cell-selex Strategy and Proceduresmentioning
confidence: 99%
“…Length of the random region of the starting library is normally between 20 to 40 bp [11]. Modified nucleotides could be also included in the library, which may greatly broaden the range of possible sequences and probably enhance their in vivo stability or nuclease resistance [24]. The design of the conserved primer regions is also important as improper design would introduce unspecific products in PCR.…”
Section: Cell-selex Strategy and Proceduresmentioning
confidence: 99%
“…2'F, 2'OMe, and 2'NH 2 nucleotides have been incorporated into RNA aptamers with Y639F mutant T7 RNA polymerase [1]. Other chemically modified nucleotides such as 2'-deoxy-2'-fluoroarabinonucleotide (FANA) [11], 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA)[12], and C2'-O-methyl(C2'-OMe)/ C2'-Fluorine (C2'-F) [13] have been incorporated into aptamers with engineered polymerase. The 2'-O-carbamoyl uridine (U cm ) is successfully incorporated by a wild-type T7 RNA polymerase [14].…”
Section: Chemically Modified Aptamersmentioning
confidence: 99%
“…Therminator DNA polymerase was used to extend a DNA hairpin primer/template with the modified nucleotides, permitting amplification of the DNA primer-template after selection and resynthesis of the selected TNA aptamer. Kuwahara and co-workers used a mixture of mutant and/or wild type KOD DNA polymerases to “transcribe” and “reverse transcribe” mixed LNA/DNA oligonucleotides during selection of an anti-thrombin aptamer [15,16]. Holliger and co-workers have dramatically expanded the potential of these analogs by evolving a series of TgoT DNA polymerase mutants to better “transcribe” and “reverse transcribe” these modified nucleotides, and they have used them to select HNA aptamers targeting HIV trans-activating response RNA and hen egg lysozyme [17•], a FANA aptamer targeting HIV-1 RT [18], and catalytic nucleic acids containing ANA, FANA, HNA, and CeNA moieties [19].…”
Section: Selex With Modified Rna and Dnamentioning
confidence: 99%