In Vitro Selection of Peanut Somatic Embryos on Medium Containing Culture Filtrate of Sclerotium rolfsii and Plantlet Regeneration

Yusnita, Yusnita; Widodo, Widodo; Sudarsono, Sudarsono

doi:10.1016/s1978-3019(16)30324-2

Cited by 29 publications

(31 citation statements)

References 23 publications

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“…However, suitable tools for detection, evaluation, identification and improvement of resistant clones should be designed in order to realize the benefits of such variations (Sahijram et al 2003). Crop improvement through somaclonal variation enables breeders to obtain plants tolerant to the biotic or abiotic stress, such as drought, high salinity, high or low soil pH and disease tolerance (Yusnita et al 2005). A number of cultivars have been developed through somaclonal variation in different horticultural crops for a range of useful traits, which are presented in Table 3.…”

Section: Application Of Somaclonal Variationsmentioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

343

216

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The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Therefore, it is of immense significance to assure the genetic uniformity of in vitro raised plants at an early stage. Several strategies have been followed to ascertain the genetic fidelity of the in vitro raised progenies comprising morpho-physiological, biochemical, cytological and DNA-based molecular markers approaches. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in horticultural crops, which are either difficult to breed or have narrow genetic base. In the present paper, sources of variations induced during tissue culture cycle and strategies to ascertain and confirm genetic fidelity in a variety of in vitro raised plantlets and potential application of variants in horticultural crop improvement are reviewed.

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Section: Application Of Somaclonal Variationsmentioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

343

216

View full text Add to dashboard Cite

show abstract

“…In the later case, higher percentage of somaclonal variation is more desirable. In various crops, somaclonal variation among somatic embryos has been used to identify drought tolerant (Rahayu and Sudarsono, 2015), quantitative and qualitative (Widoretno et al, 2003b) and diseases resistance variants (Yusnita et al, 2005).…”

Section: Somaclonal Variation Assessmentmentioning

confidence: 99%

EFFECTIVE CACAO SOMATIC EMBRYO REGENERATION ON KINETIN SUPPLEMENTED DKW MEDIUM AND SOMACLONAL VARIATION ASSESSMENT USING SSRs MARKERS

Ajijah¹,

Hartati²,

Rubiyo³

et al. 2016

Agrivita.J.Agr.Sci

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This study aimed to develop the cacao (Theobroma cacao L.) in vitro regeneration system through somatic embryogenesis on kinetin supplemented DKW medium and somaclonal variation assessment using SSR markers. Callus were initiated from basal petal and staminoid explants cultured on callus induction (CI) medium contained DKW basalt salts and kinetin:2,4-D ratios of 1:15.5, 1:7.8 or 1:3.9 and then transferred onto secondary callus growth (SCG) medium contained WPM basalt salts and kinetin:2,4-D ratios of 1:7.8 or 1:3.9. The calli were then subsequently transferred onto embryo development medium contained DKW basal salts with or without the addition of amino acids, adenine or activated charcoal for the formation of somatic embryos. Nine cacao genotypes were tested for their ability to develop somatic embryos. Results of this study indicated DKW medium supplemented with Kinetin in combination with 2,4-D effectively induced cacao somatic embryogenesis. The highest somatic embryos formation was abtained from kinetin:2,4-D ratio of 1:3.9 and 1:7.8 in CI and SCG medium respectively. Cacao genotype responses were highly explant type dependent. The developed method resulted in a high percentage of somatic embryo formation (5.6-66.7%), germination (50%) and plantlet conversion (65%) and a medium percentage of somaclonal variations based on SSRs marker analysis.

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“…In addition, Tran et al (2016) reported that there were six QTL (Quantitative Trait Loci) linked to sensitivity to toxin filtrate of C. cassiicola detected on five chromosomes of rubber plant, suggesting that cassiicolin is the main effector in pathogenicity. Using of the toxin filtrate of phytoptahogenic as a selection tool has also been reported on several pathogen such as Sclerotium rolfsii (Yusnita et al, 2009), Phytophthora parasitica (Savita et al, 2011) and Fusarium oxysporum (Oubraim et al, 2016). It caused that the detection of virulence may be more emphased on the genetic factors and minimizing the influence of environmental factors.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity and rDNA-ITS sequence analysis of the Corynespora cassiicola isolates from rubber plantations in Indonesia

Oktavia¹,

Kuswanhadi²,

Widodo

et al. 2017

Emir J Food Agric

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Corynespora cassiicola is an important pathogen causing Corynespora leaf fall disease on the rubber tree. Analysis of virulence, rDNA-ITS sequence and identification of cas gene were undertaken to investigate the effect of agro-climatic conditions and host rubber clones to virulence of 23 C. cassiicola isolates on six clones (RRIC 100, BPM 24, BPM 1, PB 260, GT 1 and RRIM 600) and to observed the evolution pattern of C. cassiicola isolates. Virulence analysis showed that there is an interaction between isolates and clones, indicating the differences of each isolates virulence to six clones. Agro-climatic conditions and host clones influenced the virulence of isolates and rDNA-ITS sequence variation. Analysis of rDNA-ITS showed that there were three of Single Nucleotide Polymorphism (SNP) that separated of isolates into 5 haplotypes. Most of the isolates belonging to the haplotype 1. Phylogenetic analysis indicated that C. cassiicola have a high diversity, however the clustering did not indicate country and host species of isolates. This information will be very useful on developing strategies of disease management and rubber plant breeding towards resistance to CLF disease.

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In Vitro Selection of Peanut Somatic Embryos on Medium Containing Culture Filtrate of Sclerotium rolfsii and Plantlet Regeneration

Cited by 29 publications

References 23 publications

Somaclonal variations and their applications in horticultural crops improvement

Somaclonal variations and their applications in horticultural crops improvement

EFFECTIVE CACAO SOMATIC EMBRYO REGENERATION ON KINETIN SUPPLEMENTED DKW MEDIUM AND SOMACLONAL VARIATION ASSESSMENT USING SSRs MARKERS

Pathogenicity and rDNA-ITS sequence analysis of the Corynespora cassiicola isolates from rubber plantations in Indonesia

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