IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE

Abstract

The aim of this study was to observe responses of two banana cultivars 'Ambon' and 'Raja Bulu' on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets. Sterilized explants were cultured on initiation medium (MS with 1mg L -1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L -1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals. Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana 'Ambon Kuning' showed higher regenerative capacity compared to 'Raja Bulu', producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L -1 BA, producing 40.7 propagules for 'Ambon Kuning', and 12.3 propagules for 'Raja Bulu' per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.

Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review

“…Each cultivar appears to be reactive to an optimal concentration of benzylaminopurine, beyond which the rate of proliferation cannot be increased and may even decrease. Concerning the stem meristematic explants, the concentration of 22.2 μM was indicated by several studies as optimal for the proliferation of most banana and plantain cultivars [42], [41], [45], [46]. Though, other values have been reported in other studies (Table I).…”

APPLICATION OF BENZYLADENINE (BA) IN THE FORM OF LANOLIN PASTE ON FLOWER-STALK BUDS SUCCESSFULLY INDUCED RE-BLOOMING OF HYBRID Phalaenopsis

“…Namun pertumbuhan mata tunas menjadi keiki atau malai bunga baru dipengaruhi oleh suhu lingkungan dan status nutrisi tanaman (Blanchard et al, 2007). Zat pengatur tumbuh benziladenin (BA) dari golongan sitokinin telah terdokumentasi sebagai perangsang pembungaan pada beberapa spesies anggrek, misalnya Dendrobium hibrida (Bahri, 2012), Phalaenopsis hibrida (Wu dan Chang, 2012); dan juga sebagai perangsang pecah dan tumbuhnya mata tunas aksilar berbagai tanaman, baik pada sistem kultur in vitro (Yusnita et al, 2015;Bihst, 2015) maupun pada level tanaman utuh (Afrianti, 2009). Namun, konsentrasi efektif BA sangat bergantung pada jenis tanaman, organ tanaman target, dan metode aplikasinya.…”

“…dilaporkan mempunyai pengaruh fisiologis memacu pembentukan tunas pisang in vitro (Yusnita et al, 2015) dan merangsang pembungaan pada beberapa spesies tanaman, seperti mawar (Kanchanapoom et al, 2009;Wang et al, 2000), Liatris spicata L.…”

In Vitro Growth Responses of Ornamental Bananas (Musa sp.) as Affected by Light Sources

“…However, many diseases, such as black sigatoka, fusarium wilt, banana bunchy top virus, burrowing nematodes, and banana weevil borer [15], are associated with traditional propagation techniques [16][17][18]. Therefore, micropropagation has become a common practice for the production of bananas, thus assisting with the production of clean, disease-free plant material [16,19,20]. The tissue culture of bananas also allows large-scale mass in vitro clonal propagation of elite banana varieties, free of diseases.…”