In vitro shoot regeneration of Stereospermum suaveolens DC. using cotyledonary node and nodal explants

Trivedi, Darshini; Joshi, Aruna

doi:10.3329/ptcb.v24i2.23556

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…The length of shoots was observed to increase with increasing concentration of Kinetin (0.5-1.5 mg.L -1 ). Similar finding was observed in Stereospermum suaveolens (Trivedi & Joshi 2014) when KN supplemented medium with higher concentrations form long healthy shoots with large leaves. It is evident from the study that micro shoots failed to exhibit high shoot elongation on media with two different levels of KN (2.0, 2.5 mg.L -1 ).…”

Section: Discussionsupporting

confidence: 74%

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In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

Bindu¹,

Dharmapal²,

Udayan³

et al. 2017

Trop. Plant Res.

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Pueraria tuberosa, commonly known as Indian kudzu or 'Vidari' is an important medicinal plant belonging to the family Fabaceae. The tubers of this plant are an important constituent in many Ayurvedic formulations as a restorative tonic, immune booster and antiageing. Annual demand of Vidari by the Ayurvedic industry is 135 tonnes and industry is facing a severe scarcity of this raw material. Micropropagation technology offers large-scale production of disease-free, quality planting materials for pharmaceutical industries. A successful protocol for the in vitro propagation of P. tuberosa has been achieved by using nodal segments as explants. Multiple shoot induction and proliferation was obtained in Murashige and Skoog medium supplemented with 0.5 mg.L -1 BAP, 0.5 mg.L -1 KN and 2% glucose. MS medium with 1.5 mg.L -1 KN favoured maximum shoot elongation. A maximum number of roots with the highest percentage of rooting was observed on half strength MS media supplemented with 0.5 mg.L -1 IBA. Elongated shoots in half strength basal MS medium induced roots in 14 days of culture. Regenerated shoots with well-developed roots were successfully hardened with 80% survival.

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Section: Discussionsupporting

confidence: 74%

“…The cytokinins BAP and KN were effective in terms of shoot induction from nodal explants of Stereospermum suaveolens (G. Don) DC. (Trivedi & Joshi 2014) and Costus speciosus (Raghu et al 2006). The combinations of 0.5 mg.L -1 BA and 0.5 mg.L -1 KN proved to be most effective for culture initiation with healthy shoots (Shubha et al 2016).…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

Bindu¹,

Dharmapal²,

Udayan³

et al. 2017

Trop. Plant Res.

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“…Thus initially, seed germination studies were carried out to determine a suitable substrate in which many seedlings can be developed (Trivedi, Joshi 2014a). Utilising seedling (cotyledonary node) explants and nodal explants, preliminary studies on effect of a single cytokinin on in vitro regeneration from these explants had been carried out (Trivedi, Joshi 2014b). Therefore, further studies to evaluate the synergistic effect of cytokinins on the nodal explants was necessary, to develop a complete protocol for the in vitro propagation of this important species.…”

Section: A a B B C C D D E E F F G G H H I Imentioning

confidence: 99%

Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

Trivedi,

Joshi

2021

EEB

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The Stereospermum suaveolens DC. is one of the important medicinal tree species and also one of the important constituents of Dashmoolarisht. Owing to its importance an attempt was made to perform in vitro regeneration from nodal explants. Cultures were established by placing the nodal explants on Murashige and Skoog's (MS) and Woody Plant media fortified with different combinations of cytokinins. The in vitro shoots developed were excised into single in vitro nodes and then utilised for multiplication of shoots. Among the various combinations tested in both of the media, the MS medium fortified with benzylaminopurine (8 µM) + thidiazuron (0.2 µM) proved to be effective in inducing a maximum of 10.2 number of shoots by the end of the fifth subculture. In vitro rooting of shoots was best achieved in ½ MS liquid medium fortified with indolebutyric acid (2.5µM), with the highest number of roots (9.3) in 100% cultures. Well rooted in vitro plantlets were successfully hardened under greenhouse conditions, resulting in 100% survival of plants after four weeks of transfer to cocopeat/soil substrate.

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In Vitro Micropropagation from Nodal Explants of the Medicinal Plant Lysimachia nummularia L.

Doğan¹

2018

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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Lysimachia nummularia L. Avrupa ve Asya'da çeşitli hastalıkların tedavisinde geleneksel olarak kullanılan önemli bir bitkidir. İçerdiği değerli biyoaktif bileşiklerden dolayı farmasötik alanda oldukça önemli bir yere sahiptir. Bu çalışmada, L. nummularia'nın doku kültürü teknikleri ile üretimi araştırılmıştır. L. nummularia'nın boğum eksplantları farklı konsantrasyonlarda 6-Benzilaminopurin (BAP)'i tek olarak veya İndol-3-Bütirik Asit (IBA) ile kombinasyonunu içeren Murashige ve Skoog (MS) besin ortamında kültüre alınmıştır. Genel olarak her iki hormon denemelerinde yüksek sürgün rejenerasyon yüzdeleri elde edilmiştir. Maksimum eksplant başına sürgün sayısı (12.27 adet) 1.6 mg L-1 BAP içeren MS besin ortamında, ardından 0.8 mg L-1 BAP + 0.1 mg L-1 IBA içeren MS besin ortamında (11 adet) elde edilmiştir. En düşük sürgün sayısı her iki hormon uygulamasında da 0.05 mg L-1 BAP içeren MS besi ortamında tespit edilmiştir. Kültür ortamında BAP konsantrasyonunun artması ile sürgün sayısı artış göstermiştir. En uzun sürgünler 0.1 mg L-1 BAP eklenmiş MS ortamında (4.6 cm) ve 0.1 mg L-1 BAP + 0.1 mg L-1 IBA eklenmiş MS ortamında (4.52 cm) kaydedilmiştir. En kısa sürgün uzunlukları 1.6 mg L-1 BAP eklenmiş MS ortamında tespit edilmiştir. Kültür ortamlarındaki rejenere sürgünler yoğun kök oluşturdukları için ayrıca köklendirme çalışması yapılmamıştır. Köklü bitkiler başarılı şekilde akvaryum ortamına alıştırılmıştır. Sonuç olarak L. nummularia'nın doku kültürü teknikleri ile üretimi için en iyi hormon 1.6 mg L-1 BAP olarak tespit edilmiştir. Bu çalışma, L. nummularia'nın doku kültürü ile çoklu üretimine imkan sağlayarak, bu bitkiden değerli bitkisel metabolitlerin yüksek miktarlarda elde edilmesine yardımcı olabilir. Böylece gıda ve farmasötik sektörlere katkı sunulabilir.

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In vitro shoot regeneration of Stereospermum suaveolens DC. using cotyledonary node and nodal explants

Cited by 3 publications

References 29 publications

In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

In vitro propagation of Pueraria tuberosa (Roxb. ex Willd.) DC.

Synergistic effect of cytokinins on in vitro propagation of Stereospermum suaveolens using nodal explants

In Vitro Micropropagation from Nodal Explants of the Medicinal Plant Lysimachia nummularia L.

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