2018
DOI: 10.17519/apiculture.2018.11.33.4.277
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In vitro Skin Irritation Test of Honeypolis using Human Skin Model

Abstract: 277Ethanol extracted propolis (EEP) was mixed with honey (honeypolis) to dissolve well in water and in vitro skin irritation test was conducted. In vitro method is designed to predict and classify the skin irritation potential of a chemical by assessment of its effect on EpiDerm TM , a reconstituted threedimensional human epidermis model. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after a 60 min exposure period. In this study unde… Show more

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