1994
DOI: 10.1038/nsb0894-502
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In vitro splicing of concanavalin A is catalyzed by asparaginyl endopeptidase

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Cited by 70 publications
(88 citation statements)
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“…We have to keep in mind, however, that all proteases are theoretically able to catalyze peptide ligation. Peptide splicing has already been described in plants and was found, in some cases, to be catalyzed by vacuolar asparaginyl endopeptidase (52,53). Because this protease is involved in the production of antigenic peptides presented by MHC class II molecules (54), future studies may reveal the existence of spliced antigenic peptides that are not produced by the proteasome.…”
Section: Discussionmentioning
confidence: 95%
“…We have to keep in mind, however, that all proteases are theoretically able to catalyze peptide ligation. Peptide splicing has already been described in plants and was found, in some cases, to be catalyzed by vacuolar asparaginyl endopeptidase (52,53). Because this protease is involved in the production of antigenic peptides presented by MHC class II molecules (54), future studies may reveal the existence of spliced antigenic peptides that are not produced by the proteasome.…”
Section: Discussionmentioning
confidence: 95%
“…This process involves proteolytic cleavage of the precursor at an internal site, resulting in the and fragments. These fragments are religated in an inverse DNA-coded order, producing the active chain ( = + ; Carrington et al, 1985;Chrispeels et al, 1986;Min & Jones, 1994). The and fragments observed in the SDS-PAGE (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Although applications of reverse proteolysis have been limited due to paucity of knowledge about mechanisms governing such reactions, examples of protease-catalyzed splicing of proteins keep emerging. The recent demonstrations of subtilisin-mediated regeneration of triose phosphate isomerase from four fragments (Vogel & Chmielewski, 1994) and splicing of concanavalin A by asparaginyl endopeptidase (Min & Hugh Jones, 1994) are noteworthy. The accumulating examples of reverse proteolysis suggest that proteases have enormous synthetic potential, which can be fully exploited only when the structural principles governing protease-catalyzed ligation reactions are completely deciphered.…”
Section: Discussionmentioning
confidence: 99%