In vitro stimulation by long-acting thyroid stimulator of thyroid glucose oxidation and 32P incorporation into phospholipids

Field, James B.; Remer, Adrienne; Bloom, Gail; Kriss, Joseph P.

doi:10.1172/jci105847

Cited by 20 publications

(3 citation statements)

References 25 publications

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“…Kriss reported that LATS sometimes increased glucose oxidation in dog liver and spleen slices, but the results were not as consistent as when thyroid slices were used (10). It has also been reported that LATS stimulates lipolysis in adipose tissue, an effect similar to that of TSH (26).…”

Section: Discussionmentioning

confidence: 92%

Effects of Long-Acting Thyroid Stimulator on Thyrotropin Stimulation of Adenyl Cyclase Activity in Thyroid Plasma Membranes

Yamashita¹,

Field²

1972

J. Clin. Invest.

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A B S T R A C T Both thyroid-stimulating hormone (TSH) and long-acting thyroid stimulator (LATS) stimulated adenyl cyclase activity in plasma membranes obtained from bovine thyroid glands. The stimulation induced by LATS was much less than that obtained with maximal amounts of TSH. LATS inhibited TSH stimulation of adenyl cyclase activity while an equivalent amount of normal human v-globulin did not influence basal or TSHstimulated activity. The inhibition by LATS appeared to be noncompetitive and was greatest when the plasma membranes were initially exposed to LATS for 30 min at 0C before being incubated with TSH for 10 min at 370C. Inhibition could still be demonstrated when the plasma membranes were incubated for 30 min at 00C with TSH before the addition of LATS. Prolonging the period of incubation of plasma membranes with LATS from 30 to 60 min did not augment the stimulation of adenyl cyclase or increase the inhibition of the effect of TSH. Papain digests of LATS also increased adenyl cyclase activity of thyroid plasma membrane and inhibited the stimulation induced by TSH. The inhibitory effect of LATS was not completely specific for TSH and thyroid plasma membranes since glucagon stimulation of adenyl cyclase in hepatic plasma membranes was also inhibited, but to a lesser extent. In contrast to the results obtained with thyroid plasma membranes, LATS did not influence basal adenyl cyclase activity in hepatic plasma membranes. Furthermore equivalent amounts of normal human a-globulin also decreased glucagon stimulation of adenyl cyclase activity in plasma membranes obtained from liver. The present data suggest that LATS stimulation of adenyl cyclase in thyroid plasma membranes might be due to a change in the membrane configuration rather than binding to a specific receptor site. Such modification of the membrane structure could

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Section: Discussionmentioning

confidence: 92%

Effects of Long-Acting Thyroid Stimulator on Thyrotropin Stimulation of Adenyl Cyclase Activity in Thyroid Plasma Membranes

Yamashita¹,

Field²

1972

J. Clin. Invest.

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“…Similarly, biochemical studies have shown that LATS stimulates glucose oxidation (Scott et Shishiba et al, 1970) and increases both the uptake of 32P into phospholipid and RNA synthesis (Field, 1968;Ochi and DeGroot, 1968).…”

Section: Mode Of Action Of Latsmentioning

confidence: 93%

The Long-Acting Thyroid Stimulator

Munro

1971

Current Topics in Experimental Endocrinology

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It is sixteen years since Adams and Purves (1956) first reported the detection of the long-acting thyroid stimulator (LATS) in the serum of patients with thyrotoxicosis. This discovery provoked a controversy about the significance of LATS in the pathogenesis of Graves' disease, which still continues. Because LATS activity can be recovered with yG-immunoglobulin (McKenzie, 1962;Adams and Kennedy, 1962;Meek et al., 1964;Kriss et al., 1964) and absorbed by thyroid subcellular particles or extracts (Kriss et al., 1964;Beall and Solomon, 1966;Dorrington et al., 1966a) it has been suggested that LATS may be a member of the group of autoantibodies to various thyroid components which have been recognised in disturbances of human thyroid function (Doniach and Roitt, 1957) including thyrotoxicosis. It must be acknowledged that the hypothesis that LATS-yG is a thyroid autoantibody with thyroid stimulating properties has no precedent in the pathogenesis of other autoimmune diseases. For detailed discussion of the scientific background to this subject the reader may consult several recent reviews (Dorrington and

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“…The prototype of organotypic tissue slice culture was developed by Dr. Harford and coworkers in the 1950s 28 . This technique was initially utilized for the assessment of pharmacological efficacy around 1970 29 , 30 . The expediting evolvement of immunotherapy facilitates the application of this technique to evaluate the immune checkpoint blockade, and adoptive cellular therapy response on tumors in recent years 31 , 32 ( Figure 1 ).…”

Section: Organotypic Tissue Slice Culturesmentioning

confidence: 99%

Recent advances in organotypic tissue slice cultures for anticancer drug development

Deng¹

2022

Int. J. Biol. Sci.

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Organotypic tissue slice culture is established from animal or patient tissues and cultivated in an in vitro ecosystem. This technique has made countless contributions to anticancer drug development due to the vast number of advantages, such as the preservation of the cell repertoire and immune components, identification of invasive ability of tumors, toxicity determination of compounds, quick assessment of therapeutic efficacy, and high predictive performance of drug responses. Importantly, it serves as a reliable tool to stratify therapeutic responders from nonresponders and select the optimal standard-ofcare treatment regimens for personalized medicine, which is expected to become a potent platform and even the gold standard for anticancer drug screening of individualization in the near future.

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In vitro stimulation by long-acting thyroid stimulator of thyroid glucose oxidation and 32P incorporation into phospholipids

Cited by 20 publications

References 25 publications

Effects of Long-Acting Thyroid Stimulator on Thyrotropin Stimulation of Adenyl Cyclase Activity in Thyroid Plasma Membranes

Effects of Long-Acting Thyroid Stimulator on Thyrotropin Stimulation of Adenyl Cyclase Activity in Thyroid Plasma Membranes

The Long-Acting Thyroid Stimulator

Recent advances in organotypic tissue slice cultures for anticancer drug development

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